Long acting atrial natriuretic peptide (la-anp) and methods for use thereof

Abstract

Materials and Methods related to making and using natriuretic polypeptides having a mutation that results in an extended carboxy terminus.

1 . A method for treating a cardiac arrhythmia in a mammal in need thereof, comprising administering to the mammal a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:13, or the amino acid sequence set forth in SEQ ID NO:13 having less than five amino acid additions, subtractions, and substitutions. 
     
     
         2 . The method of  claim 1 , wherein the polypeptide comprises an amino acid sequence present in an ANP polypeptide. 
     
     
         3 . The method of  claim 1 , wherein the polypeptide comprises an amino acid sequence present in a BNP polypeptide. 
     
     
         4 . The method of  claim 1 , wherein the polypeptide comprises an amino acid sequence present in an CNP polypeptide. 
     
     
         5 . The method of  claim 1 , wherein the polypeptide comprises an amino acid sequence present in an DNP polypeptide. 
     
     
         6 . The method of  claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:13. 
     
     
         7 . The method of  claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:13 having less than five amino acid additions, subtractions, and substitutions. 
     
     
         8 . The method of  claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:13 having less than four amino acid additions, subtractions, and substitutions. 
     
     
         9 . A method for treating a cardiac arrhythmia in a mammal in need thereof, comprising administering to the mammal a nucleic acid encoding a polypeptide that comprises the amino acid sequence set forth in SEQ ID NO:13, or the amino acid sequence set forth in SEQ ID NO:13 having less than five amino acid additions, subtractions, and substitutions. 
     
     
         10 . A method for diagnosing a cardiac arrhythmia in a mammal, comprising providing a biological sample from the mammal and assessing the biological sample to determine whether the biological sample comprises a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:13 and, if the biological sample comprises the polypeptide, diagnosing the mammal as having a cardiac arrhythmia. 
     
     
         11 . A method for determining whether a mammal is predisposed to develop a cardiac arrhythmia in a mammal, comprising providing a biological sample from the mammal and assessing the biological sample to determine whether the biological sample comprises (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:13 or (b) a nucleic acid encoding the polypeptide, and, if the biological sample comprises the polypeptide or nucleic acid, classifying the mammal as being predisposed to develop a cardiac arrhythmia or, if the biological sample does not comprise the polypeptide or nucleic acid, classifying the mammal as not being predisposed to develop a cardiac arrhythmia. 
     
     
         12 - 19 . (canceled)