Compositions comprising viruses and methods for concentrating virus preparations

Abstract

A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

1 . A composition comprising from 1×10 9  to 1×10 13  particles/ml of an adenovirus, about 20 to 200 mg/ml glycerol, about 5 to 25 mg/ml of sucrose, an aqueous buffer system which maintains a pH range from about 7.3 to about 8 at a temperature range from about 2° C. to 27° C. and which comprises from 0.5 to 10 mg/ml sodium phosphate monobasic dihydrate and from about 0.5 to 10 mg/ml tromethamine, and which further comprises magnesium chloride in a concentration of about 0.1 to 1 mg/mL. 
     
     
         2 . The composition of  claim 1 , wherein the virus is a recombinant virus. 
     
     
         3 . The composition of  claim 1  which further comprises a diluent comprising water. 
     
     
         4 . The composition of  claim 1  further comprising a surfactant. 
     
     
         5 . The composition of  claim 4 , wherein the surfactant is a polyoxyethylenesorbitan. 
     
     
         6 . The composition of  claim 5 , wherein the polyoxyethylenesorbitan is polysorbate 80. 
     
     
         7 . The composition of  claim 6 , wherein the polysorbate 80 is in a concentration of about 0.03 to 0.3 mg/mL.