Methods and systems for uniform enrichment of genomic regions

Abstract

The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.

1 . A composition comprising a solid support and a plurality of nucleic acid probes immobilized on said solid support, wherein each of said plurality of immobilized nucleic acid probes provides for uniform hybridization among a plurality of target nucleic acid sequences, and wherein the frequency of individual immobilized nucleic acid probe sequences corresponds to the frequency of the corresponding plurality of target nucleic acid sequences. 
     
     
         2 . A kit for performing uniform enrichment of target nucleic acid sequences comprising one or more containers, wherein said one or more containers comprises:
 a) a solid support comprising immobilized nucleic acid probes, wherein said probes are selected from a group consisting of a plurality of probes hybridizable to a plurality of target nucleic acid sequences and wherein said probes provide for uniform enrichment of said plurality of target nucleic acid sequences, and   b) one or more reagents for performing hybridizations, washes, and elution of target nucleic acid sequences.