Recombinant proteins from filoviruses and their use

Abstract

Filovirus subunit protein immunogens are produced using a recombinant expression system and combined with one or more adjuvants in immunogenic formulations. The subunit proteins include GP95, GP-FL, VP40, VP24, and NP derived from Ebola Virus and Marburg Virus. Adjuvants include saponins, emulsions, alum, and dipeptidyl peptidase inhibitors. The disclosed immunogenic formulations are effective in inducing strong antibody responses directed against individual Filovirus proteins and intact Filovirus particles as well as stimulating cell-mediated immune responses to the Filoviruses.

1 .- 22 . (canceled) 
     
     
         23 . An immunogenic composition comprising a recombinant Filovirus VP40 protein subunit, wherein the protein subunit is expressed and secreted as a soluble protein from stably transformed insect cells. 
     
     
         24 . An immunogenic composition comprising a recombinant Filovirus VP24 protein subunit, wherein the protein subunit is expressed and secreted as a soluble protein from stably transformed insect cells. 
     
     
         25 . An immunogenic composition comprising a recombinant Filovirus NP protein subunit, wherein the protein subunit is expressed and secreted as a soluble protein from stably transformed insect cells. 
     
     
         26 .- 49 . (canceled)