Blood coagulation time prolonging agent

Abstract

Provided is a reagent, which prolongs a blood coagulation time sufficiently and enhances an optical change, thereby enabling a correct and high-sensitive blood coagulability test. The present invention provides a blood coagulation time prolonging agent, including, as an active ingredient, a guanidine compound represented by the following formula (1) or an acid addition salt thereof (in the formula, R 1 represents a hydrogen atom, an amino group, or an alkyl group which may have a substituent).

1 . A blood coagulation time prolonging agent, comprising, as an active ingredient, a guanidine compound represented by the following formula (1) or an acid addition salt thereof: 
       
         
           
           
               
               
           
         
         where R 1  represents a hydrogen atom, an amino group, or an alkyl group which may have a substituent. 
       
     
     
         2 . The blood coagulation time prolonging agent according to  claim 1 , wherein the blood coagulation time prolonging agent comprises a blood coagulation time prolonging agent for measuring fibrinogen. 
     
     
         3 . The blood coagulation time prolonging agent according to  claim 1  or  2 , wherein R 1  represents a hydrogen atom, an amino group, an alkyl group, an alkyl group having a carboxy group and an amino group as substituents thereof, or an alkyl group having an alkoxycarbonyl group and an amino group as substituents thereof. 
     
     
         4 . A blood coagulation activator-containing reagent, comprising the blood coagulation time prolonging agent according to any one of  claims 1 . 
     
     
         5 . The blood coagulation activator-containing reagent according to  claim 4 , wherein the blood coagulation activator-containing reagent contains thrombin as a blood coagulation activator and is used for measuring fibrinogen. 
     
     
         6 . A specimen diluent for measuring blood coagulability, comprising the blood coagulation time prolonging agent according to any one of  claims 1 . 
     
     
         7 . The specimen diluent for measuring blood coagulability according to  claim 6 , wherein the specimen diluent for measuring blood coagulability comprises a specimen diluent for measuring fibrinogen. 
     
     
         8 . A use of a guanidine compound represented by the following formula (1) or an acid addition salt thereof for prolonging a blood coagulation time: 
       
         
           
           
               
               
           
         
         where R 1  represents a hydrogen atom, an amino group, or an alkyl group which may have a substituent. 
       
     
     
         9 . The use according to  claim 8 , for prolonging a blood coagulation time in fibrinogen measurement. 
     
     
         10 . The use according to  claim 8  or  9 , wherein R 1  represents a hydrogen atom, an amino group, an alkyl group, an alkyl group substituted with a carboxy group and an amino group, or an alkyl group substituted with an alkoxycarbonyl group and an amino group. 
     
     
         11 . The use according to any one of  claims 8 , wherein the guanidine compound or the acid addition salt thereof is used in combination with a blood coagulation activator. 
     
     
         12 . The use according to  claim 11 , wherein the blood coagulation activator comprises thrombin. 
     
     
         13 . The use according to any one of  claims 8 , wherein the guanidine compound or the acid addition salt thereof is used in combination with a specimen diluent for measuring blood coagulability. 
     
     
         14 . The use according to  claim 13 , wherein the specimen diluent comprises a specimen diluent for measuring fibrinogen. 
     
     
         15 . A method of prolonging a blood coagulation time, comprising adding to a plasma specimen a guanidine compound represented by the following formula (1) or an acid addition salt thereof: 
       
         
           
           
               
               
           
         
         where R 1  represents a hydrogen atom, an amino group, or an alkyl group which may have a substituent. 
       
     
     
         16 . The method according to  claim 15 , wherein R 1  represents a hydrogen atom, an amino group, an alkyl group, an alkyl group substituted with a carboxy group and an amino group, or an alkyl group substituted with an alkoxycarbonyl group and an amino group. 
     
     
         17 . The method according to  claim 15 , wherein the addition of the guanidine compound or the acid addition salt thereof is carried out in combination with a specimen diluent for measuring blood coagulability. 
     
     
         18 . The method according to  claim 17 , wherein the specimen diluent comprises a specimen diluent for measuring fibrinogen. 
     
     
         19 . The method according to  claim 15 , wherein the addition of the guanidine compound or the acid addition salt thereof is carried out in combination with a blood coagulation activator. 
     
     
         20 . The method according to  claim 19 , wherein the blood coagulation activator comprises a thrombin-containing reagent. 
     
     
         21 . A method of measuring blood coagulability, comprising the following steps of:
 preparing a reaction solution containing a plasma specimen, a blood coagulation activator, the guanidine compound or the acid addition salt thereof according to  claim 15 ; and   measuring a coagulation time of the reaction solution.