Method and apparatus for analyzing vitamin e in lipoproteins

Abstract

A method of analyzing vitamin E components in a lipoproteinby subjecting a lipoprotein-containing sample to ion exchange chromatography to separate the lipoprotein, reacting the separated lipoprotein to a pretreating solution containing an organic solvent and a surfactant to liberate vitamin E components, and then subjecting the liberated vitamin E components to reverse phase chromatography. Also described is a method of judging various pathological conditions such as the pathological conditions of diabetes, the risks of coronary artery diseases, and the pathological conditions of myocardial infarction using levels of vitamin E components in the lipoprotein as an index.

1 .- 17 . (canceled) 
     
     
         18 . An analytical apparatus comprising a sample feeding part for collecting a given amount of a sample, an ion exchange chromatography part equipped with an ion exchange column, a reagent mixing part for mixing part or all of the eluted solution from the ion exchange chromatography part with the reagent, a reverse phase chromatography part equipped with a reverse phase column, a detection part for carrying out detection on the eluted solution from the reverse phase chromatography part, and a liquid delivery part for delivering the sample collected at the sample feeding part and the eluant for the ion exchange chromatography, a liquid delivery part for delivering the reagent, and a liquid delivery part for delivering the mixture of the eluted solution from the ion exchange chromatography part and the reagent, and a liquid delivery part for delivering the eluant for the reverse phase chromatography.