Composite polynucleic acid therapeutics

Abstract

Compositions comprising therapeutic polynucleic acids and method of use. The compositions comprise one or more polynucleic acid sequences having two or more features useful for treating diseases associated with cellular proliferation or growth.

What is claimed is: 
     
         1 . A method for inhibiting the growth of mammalian cells, the method consisting of contacting cells with a polynucleic acid as set forth in SEQ ID NO. 8. 
     
     
         2 . The method of  claim 1 , wherein the mammalian cells are of human origin. 
     
     
         3 . A method for treating a proliferative disease in a mammal, the method consisting of the step of administering a polynucleic acid as set forth in SEQ ID NO. 8. 
     
     
         4 . The method of  claim 3 , wherein the mammal is a human. 
     
     
         5 . The method of  claim 3 , wherein the proliferative disease is selected from the group consisting of cancer, vascular disease, inflammation, hyperproliferative disease and combinations thereof. 
     
     
         6 . The method of  claim 5 , wherein the cancer is selected from the group consisting of breast cancer, prostate cancer, and lung cancer. 
     
     
         7 . A composition for treating a disease associated with the proliferation of mammalian cells, the composition consisting of a polynucleic acid as set forth in SEQ ID NO. 8. 
     
     
         8 . The composition of  claim 7 , wherein the polynucleic acid comprises at least two auxiliary sequences that form two or more cellular structures capable of affecting the control of genetic expression. 
     
     
         9 . The composition of  claim 7 , wherein the polynucleic acid comprises at least two auxiliary sequences that form two or more cellular structures capable of affecting the maintenance of genetic integrity. 
     
     
         10 . The composition of  claim 8 , wherein the at least two auxiliary sequences share at two least bases or nucleobases. 
     
     
         11 . The composition of  claim 8 , wherein the cellular structures capable of affecting the control of genetic expression are transcription factor binding sites. 
     
     
         12 . The composition of  claim 11 , wherein transcription factor binding sites are selected from the group consisting of E-box-, EGR-, SP-, NFKB-, E2F-, STAT-, ETS-, CRE/ATF-related sequences and combinations thereof. 
     
     
         13 . The composition of  claim 8 , wherein the cellular structures capable of affecting the control of genetic expression are CpG sites associated with DNA methylation, methylated-DNA binding proteins, and immunologic response. 
     
     
         14 . The composition of  claim 9 , wherein the cellular structures associated with proteins maintaining genetic integrity are associated with DNA repair or telomere maintenance.