Neisserial vaccine compositions comprising a combination of antigens

Abstract

The present invention relates to immunogenic compositions and vaccines for the treatment and prevention of Neisserial disease. Immunogenic compositions of the invention contain combinations of antigens selected from at least two different classes of antigens including adhesins, autotransporter proteins, toxins, iron acquisitions proteins and membrane-associated protein (preferably integral outer membrane protein)s. Such combinations of antigens are able to target the immune response against different aspects of the neisserial life cycle, resulting in a more effective immune response.

1 .- 12 . (canceled) 
     
     
         13 . An immunogenic composition comprising at least one Neisserial autotransporter antigen, at least one Neisserial Fe acquisition protein antigen, and at least one Neisserial outer membrane protein antigen wherein each of the at least one Neisserial autotransporter antigen, the at least one Neisserial Fe acquisition protein antigen, and the at least one Neisseral outer membrane protein antigen is isolated and wherein said immunogenic composition comprises both a subunit composition and an outer membrane vesicle composition wherein the outer membrane vesicle composition comprises a concentration of LPS immunotype L3 that is achievable using 0.02-0.4% deoxychoate extraction. 
     
     
         14 . The immunogenic composition of  claim 13  wherein all of the Neisserial protein antigens are derived from  N. meningitidis.    
     
     
         15 . The immunogenic composition of  claim 13  further comprising one or more bacterial capsular polysaccharides or oligosaccharides. 
     
     
         16 . The immunogenic composition of  claim 15  wherein the capsular polysaccharides or oligosaccharides are derived from bacteria selected from the group consisting of:  Neisseria meningitidis  serogroup A, C, Y and W-135. 
     
     
         17 . The immunogenic composition of  claim 13  further comprising an adjuvant. 
     
     
         18 . The immunogenic composition of  claim 17 , wherein the adjuvant comprises aluminum salts. 
     
     
         19 . The immunogenic composition of  claim 18 , wherein said aluminum salt is aluminum hydroxide gel. 
     
     
         20 . The immunogenic composition of  claim 13 , wherein the outer membrane protein antigen is GNA1870. 
     
     
         21 . The immunogenic composition of  claim 13 , wherein the Neisseral autotransporter antigen is NadA. 
     
     
         22 . The immunogenic composition of  claim 13 , wherein the Neisseral Fe acquisition protein antigen is transferrin binding protein related protein.