US2012015820A1PendingUtilityA1

Methods and compositions for polypeptide engineering

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Individually held — no corporate assignee on recordPriority: Dec 18, 1996Filed: Sep 1, 2011Published: Jan 19, 2012
Est. expiryDec 18, 2016(expired)· nominal 20-yr term from priority
Inventors:Phillip A. PattenWillem P. C. Stemmer
C40B 40/02C07K 14/545C40B 40/08C12N 15/67C07K 2317/622C12N 9/86C07K 16/00C12N 15/64C12N 2795/14043C12N 15/1034C12N 7/00C12N 15/1037C07K 2317/565C12Q 1/686C12N 15/10G01N 33/6845C12N 9/1007C12N 9/0069C12Q 1/6811A61K 38/45C12N 15/52C12N 15/1058A61K 48/00C12N 15/1031C07K 14/43595C12N 2795/14143C12N 9/16G01N 33/68C12N 15/1027C07K 14/56C40B 50/06C12Q 1/6853C12Q 1/68
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Claims

Abstract

Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

Claims

exact text as granted — not AI-modified
1 . A method for evolving a protein encoded by a DNA substrate molecule comprising:
 (a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease;   (b) ligating the mixture to generate a library of recombinant DNA molecules;   (c) screening or selecting the products of (b) for a desired property; and   (d) recovering a recombinant DNA substrate molecule encoding an evolved protein.   
     
     
         2 .- 273 . (canceled)

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