US2012003269A1PendingUtilityA1

Compositions Produced Using Enteric Pathogens and Methods of Use

65
Individually held — no corporate assignee on recordPriority: Sep 19, 2003Filed: Jan 31, 2011Published: Jan 5, 2012
Est. expirySep 19, 2023(expired)· nominal 20-yr term from priority
Inventors:Daryll A. EmeryDarren E. StraubLaura Wonderling
A61K 2039/54C07K 14/245A61K 2039/545A61K 39/105A61K 2039/55C07K 14/255A61P 31/04Y10S530/825A61K 39/0275A61P 37/04A61K 2039/552A61K 39/0258A61K 2039/542C07K 14/205
65
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Claims

Abstract

The present invention provides compositions including polypeptides having the characteristics of polypeptides expressed by a reference microbe such E. coli or Salmonella . Examples of Salmonella strains that can be used include, for instance, S. enterica serovar Newport, S. enterica serovar Enteritidis, S. enterica serovar Typhimurium, and S. enterica serovar Dublin. Also provided are compositions including polypeptides having a particular molecular weight and a mass fingerprint that includes polypeptide fragments having a particular set of masses, or polypeptides having an amino acid sequence with at least about 95% identity with an amino acid sequence, wherein the polypeptide has seroreactive activity. The present invention also provides methods of making and methods of using such compositions.

Claims

exact text as granted — not AI-modified
1 . A composition comprising:
 a first isolated polypeptide having a molecular weight of about 82 kDa as measured following electrophoresis on a sodium dodecyl sulfate (SDS)-polyacrylamide gel, wherein the first isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 82 kDa obtained from  S. enterica  serovar Newport strain MS020508 after growth in low metal conditions;   a second isolated polypeptide having a molecular weight of about 80 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the second isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 80 kDa obtained from  S. enterica  serovar Newport strain MS020508 after growth in low metal conditions; and   a third isolated polypeptide having a molecular weight of about 74 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the third isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 74 kDa obtained from  S. enterica  serovar Newport strain MS020508 after growth in low metal conditions; and   a fourth isolated polypeptide having a molecular weight of about 65 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fourth isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 65 kDa obtained from  S. enterica  serovar Newport strain MS020508 after growth in low metal conditions;   
     
     
         2 - 38 . (canceled) 
     
     
         39 . A composition comprising a polypeptide comprising the amino acid sequence of SEQ ID No 1315. 
     
     
         40 . The composition of  claim 39  further comprising the amino acid sequence of SEQ ID NO:1318. 
     
     
         41 . The composition of  claim 39  further comprising amino acids 3-16 of SEQ ID NO:1328. 
     
     
         42 . The composition of  claim 39  further comprising amino acids 13-22 of SEQ ID NO:1325. 
     
     
         43 . The composition of  claim 40  further comprising amino acids 3-16 of SEQ ID NO:1328. 
     
     
         44 . The composition of  claim 40  further comprising amino acids 13-22 of SEQ ID NO:1325. 
     
     
         45 . The composition of  claim 44  further comprising amino acids 3-16 of SEQ ID NO:1328. 
     
     
         46 . The composition of  claim 41  further comprising amino acids 13-22 of SEQ ID NO:1325. 
     
     
         47 . The composition of  claim 39  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         48 . A composition comprising the amino acid sequence of SEQ ID NO:1318. 
     
     
         49 . The composition of  claim 48  further comprising amino acids 3-16 of SEQ ID NO:1328. 
     
     
         50 . The composition of  claim 49  further comprising amino acids 13-22 of SEQ ID NO:1325. 
     
     
         51 . The composition of  claim 48  further comprising amino acids 13-22 of SEQ ID NO:1325. 
     
     
         52 . The composition of  claim 48  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         53 . A composition comprising the amino acid sequence of SEQ ID NO:766. 
     
     
         54 . The composition of  claim 53  further comprising amino acids 1-8 of SEQ ID NO:769. 
     
     
         55 . The composition of  claim 53  wherein the polypeptide is covalently bound to a carrier polypeptide. 
     
     
         56 . A composition comprising the amino acid sequence of SEQ ID NO:776 and amino acids 1-13 of SEQ ID NO:793. 
     
     
         57 . The composition of  claim 56  wherein the polypeptide is covalently bound to a carrier polypeptide.

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