US2011302722A1PendingUtilityA1

Laccases and methods of use thereof at low temperature

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Assignee: ASHTON WAYNEPriority: Dec 24, 2008Filed: Dec 22, 2009Published: Dec 15, 2011
Est. expiryDec 24, 2028(~2.4 yrs left)· nominal 20-yr term from priority
Inventors:Wayne AshtonAndreas Jacobus Johanna KrouwerJoseph C. McauliffePiera M. PericuHuaming Wang
D06P 5/15D06P 5/13D06L 4/40C12N 9/0004C12N 9/0061D06P 5/158D21C 5/005D21H 17/005D21H 21/28D06P 5/137
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Claims

Abstract

Laccase enzymes and nucleic acid sequences encoding such laccase enzymes are described. The laccase enzymes may be employed in conjunction with mediators in improved methods for modifying the color of denim fabrics. Low temperature and single-bath textile processing using laccase enzymes are also described.

Claims

exact text as granted — not AI-modified
1 . A textile processing method, comprising contacting a textile with a laccase enzyme and a mediator at a temperature less than 40° C., for a length of time and under conditions sufficient to cause a color modification of the textile. 
     
     
         2 . The method of  claim 2 , wherein the color modification is selected from lightening of color, change of color, change in color cast, reduction of redeposition/backstaining, and bleaching. 
     
     
         3 . The textile processing method of  claim 1 , wherein the temperature is from about 20° C. to less than 40° C. 
     
     
         4 . The textile processing method of  claim 1 , wherein the temperature is from about 20° C. to about 30° C. 
     
     
         5 . The textile processing method of  claim 1 , wherein the textile is indigo-dyed denim. 
     
     
         6 . The textile processing method of  claim 1 , wherein the textile is sulfur-dyed denim. 
     
     
         7 . The textile processing method of  claim 1 , wherein the denim is desized and/or stonewashed prior to or simultaneously with contacting the textile with the laccase enzyme and the mediator. 
     
     
         8 . The textile processing method of  claim 1 , wherein the stonewashing and contacting the textile with the laccase enzyme and the mediator occur in the same bath. 
     
     
         9 . The textile processing method of  claim 1 , further comprising contacting the textile with a cellulase enzyme, simultaneously or sequentially with contacting the textile with the laccase enzyme and the mediator. 
     
     
         10 . The textile processing method of  claim 9 , wherein contacting the textile with the cellulase enzyme and contacting the textile with the laccase enzyme and the mediator are performed sequentially, and wherein contacting the textile with the cellulase enzyme is performed prior to contacting the textile with the laccase enzyme and the mediator. 
     
     
         11 . The textile processing method of  claim 10 , wherein contacting the textile with the cellulase enzyme and contacting the textile with the laccase enzyme and the mediator are performed sequentially in the same bath without draining the bath between contacting the textile with a cellulase enzyme and contacting the textile with the laccase enzyme and the mediator. 
     
     
         12 . The textile processing method of  claim 9 , wherein contacting the textile with the cellulase enzyme and contacting the textile with the laccase enzyme and the mediator are performed a temperature less than 40° C. 
     
     
         13 . The method of  claim 1 , wherein the laccase is a microbial laccase. 
     
     
         14 . The method of  claim 1 , wherein the laccase is from a  Cerrena  species. 
     
     
         15 . The method of  claim 1 , wherein the laccase is from  Cerrena unicolor.    
     
     
         16 . The method of  claim 1 , wherein the laccase is laccase D from  C. unicolor.    
     
     
         17 . The method of  claim 1 , wherein the laccase has an amino acid sequence that is at least 70% identical to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20. 
     
     
         18 . The method of  claim 1 , wherein the laccase has an amino acid sequence that is at least 80% identical to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20. 
     
     
         19 . The method of  claim 1 , wherein the laccase has an amino acid sequence that is at least 70% identical to SEQ ID NO: 19 or SEQ ID NO: 20. 
     
     
         20 . The method of  claim 1 , wherein the laccase has an amino acid sequence that is at least 80% identical to SEQ ID NO: 19 or SEQ ID NO: 20. 
     
     
         21 . The method of  claim 1 , wherein the laccase has an amino acid sequence that is at least 90% identical to SEQ ID NO: 19 or SEQ ID NO: 20. 
     
     
         22 . The method of  claim 1 , wherein the mediator is syringonitrile. 
     
     
         23 . The method of  claim 1 , wherein the temperature is from about 20° to about 35° C. 
     
     
         24 . The method of  claim 1 , wherein the temperature is from about 20° C. to about 23° C. 
     
     
         25 . The method of  claim 1 , wherein the temperature is the ambient temperature of tap water. 
     
     
         26 . The method of  claim 1 , wherein the laccase enzyme and the mediator are provided together in a ready-to-use composition. 
     
     
         27 . The method of  claim 1 , wherein the laccase enzyme and the mediator are provided in a solid form.

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