Amphoteric liposomes

Abstract

Amphoteric liposomes are proposed, which comprise positive and negative membrane-based or membrane-forming charge carriers as well as the use of these liposomes.

1 .- 29 . (canceled) 
     
     
         30 . An amphoteric liposome comprising at least one positive charge carrier, at least one negative charge carrier, and at least one neutral lipid, wherein the liposome has an isoelectric point of between 4 and 8, and wherein the liposome is stable at pH 4.2 and pH 7.5. 
     
     
         31 . The amphoteric liposome of  claim 30 , wherein the liposome has an isoelectric point of between 5 and 7. 
     
     
         32 . The amphoteric liposome of  claim 30 , wherein the neutral lipid is selected from the group consisting of phosphatidyl choline, phosphatidyl ethanolamine, cholesterol, tetraether lipid, ceramide, sphingolipid, and diacyl glycerol. 
     
     
         33 . An amphoteric liposome comprising at least one amphoteric charge carrier having an isoelectric point of between 4 and 8, wherein the liposome comprises at least one neutral lipid, and wherein the liposome is stable at pH 4.2 and pH 7.5. 
     
     
         34 . The amphoteric liposome of  claim 33 , wherein the amphoteric charge carrier has an isoelectric point of between 5 and 7. 
     
     
         35 . The amphoteric liposome of  claim 33 , wherein the neutral lipid is selected from the group consisting of phosphatidyl choline, phosphatidyl ethanolamine, cholesterol, tetraether lipid, ceramide, sphingolipid, and diacyl glycerol. 
     
     
         36 . The amphoteric liposome of  claim 33 , further comprising at least one anionic and/or cationic charge carrier. 
     
     
         37 . The amphoteric liposome of  claim 36 , wherein the anionic charge carrier is a weak anionic lipid. 
     
     
         38 . The amphoteric liposome of  claim 36 , wherein the cationic charge carrier is a strong cationic lipid. 
     
     
         39 . The amphoteric liposome of  claim 37 , wherein the weak anionic lipid is selected from the group consisting of cholesterol hemisuccinate (CHEMS), diacyl glycerol hemisuccinate, fatty acids and phosphatidylserine. 
     
     
         40 . The amphoteric liposome of  claim 38 , wherein the strong cationic lipid is selected from the group consisting of DOTAP, DC-Chol, DORIE, DDAB, TC-Chol, DOTMA, DOGS, (C18) 2 Gly+N,N-dioctadecylamino-glycin, CTAB, CPyC and DOEPC. 
     
     
         41 . The amphoteric liposome of  claim 36 , wherein the cationic charge carrier is a weak cationic lipid. 
     
     
         42 . The amphoteric liposome of  claim 41 , wherein the weak cationic lipid is selected from the group consisting of HisChol and MoChol. 
     
     
         43 . The amphoteric liposome of  claim 36 , wherein the anionic charge carrier is a strong anionic lipid. 
     
     
         44 . The amphoteric liposome of  claim 43 , wherein the strong anionic lipid is selected from the group consisting of cholesterol sulphate, cholesterol phosphate, phosphatidylglycerol, phosphatidic acid, phosphatidyl inositol, and cetyl phosphate. 
     
     
         45 . The amphoteric liposome of  claim 30  or  claim 33 , wherein the liposome has an average size of between 50 and 1000 nm. 
     
     
         46 . The amphoteric liposome of  claim 45 , wherein the liposome has an average size of between 70 and 250 nm. 
     
     
         47 . The amphoteric liposome of  claim 45 , wherein the liposome has an average size of between 60 and 130 nm. 
     
     
         48 . The amphoteric liposome of  claim 30  or  claim 33 , wherein the liposome comprises an active ingredient. 
     
     
         49 . The amphoteric liposome of  claim 48 , wherein the active ingredient is a protein, a peptide, a DNA, an RNA, antisense nucleotide, or a decoy nucleotide. 
     
     
         50 . The amphoteric liposome of  claim 48 , wherein at least 80 percent of the active ingredient is in the interior of the liposome.