Method for modulating muscle differentiation or regeneration

Abstract

Present invention relates to method for promoting or inhibiting muscle differentiation or regeneration using an enhancer or an inhibitor capable of modulating the binding of TAZ polypeptide with MyoD polypeptide; method for screening a substance capable of up- or down-regulating muscle differentiation or regeneration by use of interaction between TAZ polypeptide and MyoD polypeptide; an isolated peptide consisting of an amino acid sequence of SEQ ID NO. 2 which binds to and activates the MyoD polypeptide; a polynucleotide encoding the isolated peptide; and a pharmaceutical composition comprising the isolated peptide or the polynucleotide.

1 . A method for promoting muscle differentiation or regeneration, comprising administering an enhancer capable of inducing or promoting the binding of TAZ polypeptide with MyoD polypeptide to a subject or muscle cell thereof in need of muscle differentiation or regeneration. 
     
     
         2 . The method according to  claim 1 , wherein the method further comprises administering a TAZ polypeptide, a fragment thereof, or a polynucleotide encoding the same; a MyoD polypeptide, a fragment thereof, or a polynucleotide encoding the same; or a mixture thereof. 
     
     
         3 . The method according to  claim 1 , wherein the enhancer inhibits the activity of an inhibitor that suppresses or inhibits the binding of TAZ polypeptide with MyoD polypeptide. 
     
     
         4 . A method for inhibiting muscle differentiation or regeneration, comprising administering an inhibitor capable of suppressing or inhibiting the binding of TAZ polypeptide with MyoD polypeptide to a subject or muscle cell thereof in need of inhibiting muscle differentiation or regeneration. 
     
     
         5 . A method for screening a substance capable of up- or down-regulating muscle differentiation or regeneration by use of interaction between TAZ polypeptide and MyoD polypeptide. 
     
     
         6 . The method according to  claim 5 , wherein the interaction is a direct or indirect binding of TAZ polypeptide with MyoD polypeptide. 
     
     
         7 . An isolated peptide consisting of an amino acid sequence of SEQ ID NO. 2, a fragment thereof, or a variant of SEQ ID NO. 2 having 60% or more homology therewith, wherein said peptide binds to and activates the MyoD polypeptide. 
     
     
         8 . The peptide according to  claim 7 , wherein the isolated peptide has an activity of inducing muscle differentiation or regeneration. 
     
     
         9 . The peptide according to  claim 7 , wherein the isolated peptide is an amino acid region at positions 124-395 of TAZ polypeptide of SEQ ID NO. 1. 
     
     
         10 . A polynucleotide encoding the isolated peptide of  claim 7 . 
     
     
         11 . The polynucleotide according to  claim 10 , wherein the polynucleotide is in a form of expression vector. 
     
     
         12 . A pharmaceutical composition comprising the isolated peptide of  claim 7  or the polynucleotide encoding the same. 
     
     
         13 . The pharmaceutical composition according to  claim 12 , wherein the pharmaceutical composition is used for inducing muscle differentiation or regeneration, or treating muscular disease. 
     
     
         14 . The pharmaceutical composition according to  claim 13 , wherein the muscular disease is skeletal muscle disorders, cardiac muscle pathologies, or smooth muscle disorders. 
     
     
         15 . The pharmaceutical composition according to  claim 13 , wherein the muscular disease is selected from the group consisting of myopathies, myoneural conductive diseases, traumatic muscle injury, nerve injury, ischemic damage, congenital and traumatic disorders, arterial sclerosis, vascular lesions, and congenital vascular diseases.