Polypeptide marker for diagnosis of arteriosclerosis, method for detection of arteriosclerosis by using the maker or the like, and kit for diagnosis of arteriosclerosis

Abstract

Disclosed are: a polypeptide marker for diagnosing arteriosclerosis; a gene marker for diagnosing arteriosclerosis; an antibody; a probe for detecting an arteriosclerosis marker gene; a DNA microarray or a DNA chip for detecting an arteriosclerosis marker gene; a method for detecting arteriosclerosis; and a kit for diagnosing arteriosclerosis; with which an arteriosclerotic lesion can be detected with much improved accuracy. Specifically disclosed are: a polypeptide marker for diagnosing arteriosclerosis, which comprises a polypeptide having an amino acid sequence set forth in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, and 35 of the Sequence Listing, or a partial amino acid sequence thereof; a gene which encodes the amino acid sequence; a probe for detecting the gene; a DNA microarray or a DNA chip comprising the probe; an antibody bindable to the polypeptide as an antigen; a kit comprising any one of the above-mentioned items; and a method for detecting arteriosclerosis by using any one of the above-mentioned items.

1 . A polypeptide marker for diagnosing arteriosclerosis, which comprises a polypeptide having an amino acid sequence set forth in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, and 35 of the Sequence Listing, or a partial amino acid sequence thereof. 
     
     
         2 . A gene marker for diagnosing arteriosclerosis, which comprises a gene represented by a nucleotide sequence that encodes the amino acid sequence according to  claim 1 , or a partial amino acid sequence thereof. 
     
     
         3 . A gene marker for diagnosing arteriosclerosis, which comprises a gene having a nucleotide sequence set forth in any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, and 36 of the Sequence Listing, or a partial nucleotide sequence thereof. 
     
     
         4 . An antibody specifically bindable to the polypeptide according to  claim 1  as an antigen. 
     
     
         5 . A probe for detecting an arteriosclerosis marker gene, which comprises all or a part of DNA that is hybridizable with the gene according to either one of  claim 2  and  claim 3  under a stringent condition. 
     
     
         6 . The probe for detecting an arteriosclerosis marker gene according to  claim 5 , which comprises all or a part of antisense DNA for the gene according to  claim 3 . 
     
     
         7 . A DNA microarray or a DNA chip for detecting an arteriosclerosis marker gene, wherein the probe for detecting an arteriosclerosis marker gene according to  claim 5  and/or the probe for detecting an arteriosclerosis marker gene according to  claim 6  is/are immobilized on a base plate. 
     
     
         8 . A method for detecting arteriosclerosis, wherein the antibody according to  claim 4  is used to detect the expression of an arteriosclerosis marker polypeptide that is specifically bindable to the antibody, in a specimen sample. 
     
     
         9 . A method for detecting arteriosclerosis, wherein the polypeptide marker for diagnosing arteriosclerosis according to  claim 1  is used to detect the expression of an arteriosclerosis marker antibody that is specifically bindable to the polypeptide marker for diagnosing arteriosclerosis, in specimen blood. 
     
     
         10 . A method for detecting arteriosclerosis, wherein the probe for detecting an arteriosclerosis marker gene according to either one of  claim 5  and  claim 6  is used to detect the expression of an arteriosclerosis marker gene that is hybridizable with the probe for detecting an arteriosclerosis marker gene, in a specimen cell. 
     
     
         11 . A method for detecting arteriosclerosis, wherein a primer is constructed based on a nucleotide sequence set forth in any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, and 36 of the Sequence Listing, and PCR is conducted with use of the primer to detect the expression of an arteriosclerosis marker gene. 
     
     
         12 . A kit for diagnosing arteriosclerosis, including at least one item selected from the group consisting of the probe for detecting an arteriosclerosis marker gene according to either one of  claim 5  and  claim 6 , the DNA microarray or the DNA chip for detecting an arteriosclerosis marker gene according to  claim 7 , and the antibody according to  claim 4 . 
     
     
         13 . A kit for diagnosing arteriosclerosis, including a polypeptide marker for diagnosing arteriosclerosis which comprises a polypeptide having an amino acid sequence set forth in any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, and 35 of the Sequence Listing, or a partial amino acid sequence thereof.