Isolated mammalian membrane protein genes; related reagents

Abstract

Nucleic acids encoding various lymphocyte cell proteins from mammalian, including primate, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided.

1 . A substantially pure or recombinant DCMP1 polypeptide exhibiting at least about 85% sequence identity to SEQ ID NO: 2 or 8. 
     
     
         2 . A substantially pure or recombinant DCMP2 polypeptide comprising:
 a) a polypeptide selected from:
 1) Gly Val Ser Glu Leu Gln Glu His Thr Thr Gln Lys Ala His Leu Gly His Cys Pro His Cys Pro Ser Val Cys Val Pro (residues 118-144 of SEQ ID NO: 4); 
 2) Gln Val Ala Thr Leu Asn Asn Asn Ala Ser Thr Glu Gly Thr Cys Cys (residues 166-181 of SEQ ID NO: 4); or 
 3) Trp Lys Pro Gly Gln Pro Asp Asn Trp Gln Gly His Gly Leu Gly (residues 263-277 of SEQ ID NO: 4); or 
   b) sequence exhibiting both:
 1) at least 17 contiguous amino acids from DCMP2v as described in SEQ ID NO: 10; and 
 2) a lack of a segment of at least 12 contiguous amino acids from FKNGPLPLQS LLQRLRWGPC HLLLWLGLGL LLLVIIC (residues 20-56 of SEQ ID NO: 4). 
   
     
     
         3 . A fusion protein comprising the polypeptide of  claim 1  or  2 . 
     
     
         4 . A binding compound which specifically binds to the polypeptide of  claim 1  or  2 . 
     
     
         5 . The binding compound of  claim 4  which is an antibody or antibody fragment. 
     
     
         6 . A nucleic acid encoding the polypeptide of  claim 1  or  2 . 
     
     
         7 . An expression vector comprising the nucleic acid of  claim 6 . 
     
     
         8 . A host cell comprising the vector of  claim 7 . 
     
     
         9 . A process for recombinatly producing a polypeptide comprising culturing the host cell of  claim 8  under conditions in which the polypeptide is expressed.