Sirna capable of inhibiting the expression of an oncogene involved in cervical cancer

Abstract

The present invention identifies the total nucleotide sequence of a novel oncogene from human, which is directly involved in such a cancerization mechanism as for cervical cancer induced by HPV infection of cervical epithelial cell and the amino acid sequence of an oncogenic protein encoded thereby, and to provide a full-length polynucleotide encoding a peptide chain of the oncogenic protein derived from the novel oncogene, which can be used for recombinant production of the oncogenic protein, and the peptide chain of the oncogenic protein produced recombinantly therewith. Specifically, the present invention provides a novel oncogene polynucleotide from human involving development of cervical cancer, comprising a nucleotide sequence encoding an amino acid sequence of SEQ ID NO: 1, particularly a polynucleotide of the nucleotide sequence of SEQ ID NO: 2.

1 . A recombinant polypeptide essentially consisting of the amino acid sequence of SEQ ID. NO: 1 or a salt thereof. 
     
     
         2 . A recombinant oncogenic protein essentially consisting of the amino acid sequence of SEQ ID NO: 1. 
     
     
         3 . An antibody being generated by immunizing a rabbit with a peptide fragment consisting of amino acids 814 to 1037 of the amino acid sequence of SEQ ID. NO: 1,
 wherein the antibody is capable of binding to the peptide fragment consisting of amino acids 814 to 1037 of the amino acid sequence of SEQ ID NO: 1.   
     
     
         4 . A kit for an antigen-antibody reaction comprising the antibody of  claim 3 , wherein the antibody of  claim 3  is used to detect an oncogenic protein consisting of the amino acid sequence of SEQ ID NO: 1.