Method for production of plant imparted with stress tolerance and use thereof

Abstract

The present invention provides a method for producing a plant with a stress tolerance, comprising the step of inhibiting, in a plant, a function of a first polypeptide including an amino acid sequence represented by any one of SEQ ID NOS: 2, 4, 6, 8, and 10. This enables developing a new technique capable of producing a plant with a stress tolerance such as a salt tolerance and a high osmotic pressure tolerance.

1 . A method for producing a plant with a stress tolerance, comprising the step of inhibiting, in a plant, a function of a first polypeptide including an amino acid sequence represented by any one of SEQ ID NOS: 2, 4, 6, 8, and 10. 
     
     
         2 . The method as set forth in  claim 1 , wherein the step of inhibiting a function of a first polypeptide is carried out in such a manner that a fusion protein of the first polypeptide and a second polypeptide which converts any transcription factor into a transcription inhibiting factor is produced in the plant. 
     
     
         3 . The method as set forth in  claim 1 , wherein the step of inhibiting a function of a first polypeptide is carried out in such a manner that expression of the first polypeptide is inhibited in the plant. 
     
     
         4 . The method as set forth in  claim 1 , wherein the stress tolerance is a salt tolerance and/or a high osmotic pressure tolerance. 
     
     
         5 . A plant, produced by a method as set forth in  claim 1 . 
     
     
         6 . The plant as set forth in  claim 5 , selected from the group consisting of a grown plant individual, a plant cell, a plant tissue, a callus, and a seed. 
     
     
         7 . A kit for producing a plant with a stress tolerance, comprising:
 a first polynucleotide including a base sequence represented by any one of SEQ ID NOS: 1, 3, 5, 7, and 9; and   a second polynucleotide encoding a functional peptide which converts any transcription factor into a transcription inhibiting factor.   
     
     
         8 . The kit as set forth in  claim 7 , wherein the first polynucleotide and the second polynucleotide are bound to each other in frame. 
     
     
         9 . A kit for producing a plant with a stress tolerance, comprising:
 an oligonucleotide set including any one of a first primer set (SEQ ID NOS: 11 and 12), a second primer set (SEQ ID NOS: 13 and 14), a third primer set (SEQ ID NOS: 15 and 16), a fourth primer set (SEQ ID NOS: 17 and 18), and a fifth primer set (SEQ ID NOS: 19 and 20); and   a polynucleotide encoding a functional peptide which converts any transcription factor into a transcription inhibiting factor.   
     
     
         10 . The kit as set forth in  claim 7 , further comprising an expression vector for expressing a target polypeptide in a plant. 
     
     
         11 . The kit as set forth in  claim 10 , further comprising reagents for introducing the expression vector into a plant cell. 
     
     
         12 . A method for imparting a plant with a stress tolerance, comprising the step of inhibiting, in a plant, a function of a polypeptide including an amino acid sequence represented by any one of SEQ ID NOS: 2, 4, 6, 8, and 10. 
     
     
         13 . A kit for imparting a plant with a stress tolerance, comprising:
 a first polynucleotide including a base sequence represented by any one of SEQ ID NOS: 1, 3, 5, 7, and 9; and   a second polynucleotide encoding a functional peptide which converts any transcription factor into a transcription inhibiting factor.   
     
     
         14 . The kit as set forth in  claim 13 , wherein the first polynucleotide and the second polynucleotide are bound to each other in frame. 
     
     
         15 . A kit for imparting a plant with a stress tolerance, comprising:
 an oligonucleotide set including any one of a first primer set (SEQ ID NOS: 11 and 12), a second primer set (SEQ ID NOS: 13 and 14), a third primer set (SEQ ID NOS: 15 and 16), a fourth primer set (SEQ ID NOS: 17 and 18), and a fifth primer set (SEQ ID NOS: 19 and 20); and   a polynucleotide encoding a functional peptide which converts any transcription factor into a transcription inhibiting factor.   
     
     
         16 . The kit as set forth in  claim 9 , further comprising an expression vector for expressing a target polypeptide in a plant. 
     
     
         17 . The kit as set forth in  claim 16 , further comprising reagents for introducing the expression vector into a plant cell.