Novel protein capable of binding to hyaluronic acid, and method for measurement of hyaluronic acid using the same

Abstract

The present invention relates to a polynucleotide encoding a protein comprising an amino acid sequence shown in SEQ ID NO: 2, wherein the protein encoded by the polynucleotide has a hyaluronic acid binding ability, the protein, a method for measuring hyaluronic acid using the protein, and a reagent kit for measuring hyaluronic acid comprising the protein as a constituent.

1 . A polynucleotide encoding a protein having a hyaluronic acid binding ability comprising an amino acid sequence shown in SEQ ID NO: 2,
 wherein the amino acid sequence has an isoleucine residue in position 130 from N-terminal of the amino acid sequence, and a residue derived from an amino acid selected from the group consisting of tyrosine, serine, threonine, cysteine, asparagine and glutamine in position 131 from N-terminal of the amino acid sequence.   
     
     
         2 . The polynucleotide according to  claim 1 , wherein the amino acid sequence is shown in SEQ ID NO: 4. 
     
     
         3 . The polynucleotide according to  claim 1 , wherein the polynucleotide encoding a protein comprising an amino acid sequence shown in SEQ ID NO: 2 is a polynucleotide comprising a nucleotide sequence shown in SEQ ID NO: 1; a base in position 390 from 5′ terminal of the polynucleotide is the base selected from T, C and A; and bases in positions 391 to 393 from 5′ terminal are nucleotide sequences selected from TAT, TAC, TCT, TCC, TCA, TCG, ACT, ACC, ACA, ACG, TGT, TGC, AAT, AAC, CAA and CAG (in this connection, A represents adenine, C represents cytosine, G represents guanine, and T represents thymine, respectively, and hereinafter same as above). 
     
     
         4 . The polynucleotide according to  claim 3 , wherein the nucleotide sequence in positions 391 to 393 is TAT. 
     
     
         5 . The polynucleotide according to  claim 3 , wherein the base in position 390 is C. 
     
     
         6 . The polynucleotide according to  claim 3 , comprising a nucleotide sequence shown in SEQ ID NO: 3. 
     
     
         7 - 12 . (canceled)