Methods for breeding glyphosate resistant plants and compositions thereof

Abstract

A plant comprising SEQ. ID. NO. 2 or a functional portion thereof, wherein SEQ ID. NO. 2 is not native to said plant. A glyphosate resistant grass of economic value comprises a nucleic acid molecule that encodes an EPSPS enzyme. In some embodiments, the nucleic acid molecule comprises a sequence of SEQ. ID. NO. 1, or a functional portion thereof. In some embodiments, the EPSPS enzyme can be a polypeptide molecule comprising an amino acid sequence that is essentially of SEQ. ID. NO. 2, or portion thereof. Embodiments include a DNA construct comprising a SEQ. ID. NO. 1 or a functional portion thereof and transgenic methods for inserting the DNA construct into a plant. Some embodiments include non-transgenic glyphosate resistant turfgrasses.

1 - 11 . (canceled) 
     
     
         12 . A plant comprising SEQ. ID. NO. 2 or a functional portion thereof, wherein said SEQ. ID. NO. 2 is not native to said plant. 
     
     
         13 . The plant according to  claim 12 , wherein said SEQ. ID. NO. 2 or a functional portion thereof is a glyphosate resistant EPSPS protein. 
     
     
         14 . The plant according to  claim 13 , wherein said glyphosate resistant EPSPS protein is encoded by SEQ. ID. NO. 1 or a functional portion thereof. 
     
     
         15 . The plant according to  claim 12 , wherein the plant is from the family Gramineae. 
     
     
         16 . The plant according to  claim 12 , wherein the plant is not a transgenic. 
     
     
         17 . The plant according to  claim 12 , wherein the plant is a perennial turfgrass. 
     
     
         18 . The seed of the plant according to  claim 12 . 
     
     
         19 . A seed mixture comprising said seed according to  claim 18 . 
     
     
         20 - 29 . (canceled) 
     
     
         30 . A glyphosate resistant grass of economic value comprising a nucleic acid molecule that encodes a glyphosate resistant EPSPS enzyme, wherein said nucleic acid molecule comprises a sequence of SEQ. ID. NO. 1, or a functional portion thereof, and is not native to said grass. 
     
     
         31 . (canceled) 
     
     
         32 . The glyphosate resistant grass of economic value according to  claim 30 , wherein said glyphosate resistant EPSPS enzyme comprises SEQ. ID. NO. 2, or a functional portion thereof. 
     
     
         33 . The glyphosate resistant grass of economic value according to  claim 30 , wherein said grass is selected from the group consisting of a glyphosate resistant ryegrass, a glyphosate resistant fescue, a glyphosate resistant forage grass, a glyphosate resistant wheat, combinations thereof, and hybrids thereof. 
     
     
         34 . The glyphosate resistant grass of economic value according to  claim 30 , wherein said grass is a hybrid comprising germplasm from a non-transgenic glyphosate resistant  Lolium rigidum.    
     
     
         35 . A seed of a glyphosate resistant grass of economic value according to  claim 30 . 
     
     
         36 . A seed mixture comprising said seed according to  claim 35 . 
     
     
         37 . A progeny of a non-transgenic glyphosate resistant grass of economic value according to  claim 30 . 
     
     
         38 . A vegetative sprig or clone of a glyphosate resistant grass of economic value according to  claim 30 . 
     
     
         39 . The glyphosate resistant grass of economic value according to  claim 30 , wherein said turf grass is in a golf course fairway, a golf course rough, a golf course tee box, a lawn, an athletic field, a park, a school yard, a roadside, a right of way, a trail, or a sod. 
     
     
         40 . A glyphosate resistant gene obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass deposited on Jan. 23, 2007. 
     
     
         41 . The gene according to  claim 40  comprising SEQ. ID NO. 1 or a functional portion thereof. 
     
     
         42 . The gene according to  claim 40  encoding at least one of a glyphosate resistant EPSPS and a glyphosate resistant GRP. 
     
     
         43 . The gene according to  claim 40  encoding a glyphosate resistant EPSPS comprising SEQ. ID. NO. 2 or a functional portion thereof. 
     
     
         44 . The gene according to  claim 40  encoding a glyphosate resistant GRP identified by applying a herbicidally effective amount of glyphosate to the germplasm and then analyzing any surviving germplasm for at least one biomarker. 
     
     
         45 . A DNA construct comprising said at least one gene according to  claim 40 . 
     
     
         46 . A method of transforming a plant, the method comprising:
 transforming a plant with the DNA construct according to  claim 45 .   
     
     
         47 . (canceled) 
     
     
         48 . A glyphosate resistant EPSPS obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass deposited Jan. 23, 2007. 
     
     
         49 . A gene encoding said glyphosate resistant EPSPS according to  claim 48 . 
     
     
         50 . A glyphosate resistant GRP protein obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass deposited Jan. 23, 2007. 
     
     
         51 . A gene encoding said glyphosate resistant GRP according to  claim 50 . 
     
     
         52 - 65 . (canceled) 
     
     
         66 . An isolated nucleic acid molecule comprising the base sequence of SEQ. ID. NO. 1, or a functional portion thereof. 
     
     
         67 . A DNA construct comprising a polynucleotide segment that has a base sequence of SEQ. ID. NO. 1, or a functional portion thereof, and that encodes SEQ. ID. NO. 2, said polynucleotide segment being operably attached to expression regulatory elements that can be used by a host cell to express an EPSPS polypeptide encoded by said polynucleotide segment. 
     
     
         68 . A recombinant DNA molecule comprising a structural DNA segment that encodes an EPSPS polypeptide comprising sequence of SEQ. ID. NO. 2, or a functional portion thereof, operably linked to expression regulatory elements that can be used by a host cell to express said EPSPS polypeptide or peptide encoded by said structural DNA segment. 
     
     
         69 . The recombinant DNA molecule according to  claim 68 , wherein said expression regulatory elements are those that can be used by a plant cell to achieve expression of said structural DNA sequence. 
     
     
         70 . The recombinant DNA molecule according to  claim 68 , wherein said expression regulatory elements include a plant promoter that is heterologous to said structural DNA sequence. 
     
     
         71 . The recombinant DNA molecule of  claim 68  further comprising a DNA sequence that is operably linked to said structural DNA segment and that encodes an amino-terminal chloroplast transit peptide. 
     
     
         72 . A method of producing a glyphosate resistant plant, the method comprising:
 inserting into the genome of a plant cell a recombinant DNA molecule comprising: a promoter that functions in plant cells, operably linked to a structural DNA sequence that encodes an EPSPS enzyme having the sequence of SEQ. ID. NO. 2, operably linked to a 3′ non-translated region that functions in plant cells to cause the addition of polyadenyl nucleotides to the 3′ end of a RNA sequence; and   regenerating from the transformed plant cell a genetically transformed plant which has increased tolerance to glyphosate herbicide as compared to an untransformed plant.   
     
     
         73 . The method according to  claim 72 , wherein said recombinant DNA molecule further comprises a DNA sequence operably linked to said structural DNA sequence encoding an amino-terminal chloroplast transit peptide.