Goodpasture Antigen Binding Protein and its Detection

Abstract

The present invention provides native Goodpasture antigen binding protein isoforms, monoclonal antibodies directed against such proteins, and methods for their use.

1 . A method for detecting urinary Goodpasture antigen binding protein (GPBP), comprising
 (a) contacting a urine sample with an antibody that binds to 91 kD GPBP as set forth in SEQ ID NO:2 and/or with an antibody that binds to 77 kD GPBP as set forth in SEQ ID NO:4, under conditions to promote selective binding of the antibody to the 91 kD GPBP and/or to the 77 kD GPBP;   (b) removing unbound urine and/or antibodies; and   (c) detecting complex formation between the antibody and the 91 kD GPBP, and/or between the antibody and the 77 kD GPBP in the urine sample.   
     
     
         2 . The method of  claim 1 , wherein the method comprises detecting native 91 kD GPBP from human urine. 
     
     
         3 . The method of  claim 1 , wherein the method comprises detecting native 77 kD GPBP from human urine. 
     
     
         4 . The method of  claim 1 , wherein the antibody is a monoclonal antibody. 
     
     
         5 . The method of  claim 1 , wherein the detecting comprises a technique selected from the group consisting of ELISA, immunoflourescence, flow cytometry, and chromatography. 
     
     
         6 . The method of  claim 1  wherein the urine sample is obtained from a human subject suspected of having a disorder selected from the group consisting of Goodpasture Syndrome and immune-complex mediated glomerulonephritis.