Methods of identifying combinations of antibodies with an improved anti-tumor activity and compositions and methods using the antibodies

Abstract

A method of identifying a combination of antibodies with a combined improved anti tumor activity is provided. The method comprising identifying at least two anti RTK antibodies capable of inducing synergistic endocytosis of the RTK in a cell expressing the RTK, thereby identifying the combination of antibodies with the combined improved anti-tumor activity.

1 . A method of identifying a combination of antibodies with a combined improved anti-tumor activity, the method comprising:
 assaying endocytosis of ErbB-1 induced by anti-ErbB-1 antibodies; and   identifying a combination of at least two antibodies of said anti-ErbB-1 antibodies having a synergism in said endocytosis of said ErbB-1, said combination of antibodies being with the combined improved anti-tumor activity.   
     
     
         2 . The method of  claim 1 , wherein said at least two antibodies bind to distinct extracellular epitopes of said ErbB-1. 
     
     
         3 . The method of  claim 1 , wherein each of said at least two antibodies bind said ErbB-1 with an affinity of at least 200 nM or lower. 
     
     
         4 . The method of  claim 1 , wherein said at least two antibodies bind said ErbB-1 with a similar affinity. 
     
     
         5 . The method of  claim 1 , wherein said at least two antibodies comprise a recombinant antibody. 
     
     
         6 . The method of  claim 5 , wherein said recombinant antibody is a bispecific antibody or a single chain antibody. 
     
     
         7 . The method of  claim 1 , wherein said at least two antibodies comprise a monoclonal antibody. 
     
     
         8 . The method of  claim 1 , wherein said at least two antibodies comprise a polyclonal antibody. 
     
     
         9 . The method of  claim 1 , wherein said at least two antibodies comprise a humanized antibody. 
     
     
         10 . The method of  claim 1 , wherein said at least two antibodies comprise an antibody fragment. 
     
     
         11 . The method of  claim 1 , wherein said at least two antibodies comprise at least a bivalent antibody.