Nucleotide sequence encoding a modulator of nf-kb

Abstract

The present invention relates to nucleotide sequences encoding a modulator of NF-κB, and to the polypeptides encoded by the nucleotide sequences. In particular, the invention relates to nucleotide sequences and the polypeptides encoded thereby, wherein the polypeptides are involved in the response to NF-κB-activating stimuli, including HTLV-1 Tax, LPS, PMA and IL-1. The invention also relates to antibodies to the modulator of NF-κB, methods of detecting modulator of NF-κB using the antibodies, methods of treatment associated with NF-κB activation and to methods of identifying compounds which modulate the activity of the modulator of NF-κB.

1 - 50 . (canceled) 
     
     
         51 . A purified polypeptide, wherein the polypeptide is a modulator of NF-κB and has the following characteristics:
 a) an apparent molecular weight of approximately 48 kD; 
 b) a pI of approximately 5.66; 
 c) containing a leucine zipper motif; and 
 d) binding to a kinase involved in the activation of NF-kB. 
 
     
     
         52 . The polypeptide of  claim 51 , which is deficient in rat 5R cell and in murine pre-B cell line 1.3E2. 
     
     
         53 . The polypeptide of  claim 51 , which is labeled with a detectable label. 
     
     
         54 . The polypeptide of  claim 53 , wherein the label is selected from the group consisting of enzymes, chemicals which fluoresce and radioactive elements. 
     
     
         55 . A pharmaceutical composition comprising the polypeptide of  claim 51  and a pharmaceutically acceptable carrier. 
     
     
         56 . A method for producing the polypeptide of  claim 51 , comprising:
 (a) culturing a unicellular host transformed with a recombinant DNA encoding the polypeptide; and   (b) isolating the polypeptide from cultured unicellular host.   
     
     
         57 . The method of  claim 56 , wherein the unicellular host is selected from group consisting of  E. coli, Pseudomonas, Bacillus, Streptomyces , yeasts, CHO, R1.1, B-W, L-M, COS 1, COS 7, BSC1, BSC40, and BMT10 cells, plant cells, insect cells, and human cells in tissue culture.