Polypeptides having Alpha-glucosidase activity and polynucleotides encoding same

Abstract

The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

1 . A method for producing fermented a malt beverage, comprising adding a polypeptide having alpha-glucosidase activity to a wort for manufacturing a fermented malt beverage, wherein the polypeptide having alpha-glucosidase activity is added prior to heat treatment of wort and the polypeptide having alpha-glucosidase activity is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to amino acids 21 to 960 of SEQ ID NO: 2; (b) a polypeptide that is encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) nucleotides 61 to 2880 of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising nucleotides 61 to 2880 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         2 . The method of  claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 95% or 97% sequence identity with amino acids 21 to 960 of SEQ ID NO: 2. 
     
     
         3 . The method of  claim 1 , wherein the polypeptide comprises or consists of SEQ ID NO: 2, or a fragment thereof having alpha-glucosidase activity. 
     
     
         4 . The method of  claim 1 , wherein the polypeptide comprises or consists of amino acids 21 to 960 of SEQ ID NO: 2. 
     
     
         5 . The method of  claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency or very high conditions with (i) nucleotides 61 to 2880 of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising nucleotides 61 to 2880 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii). 
     
     
         6 . The method of  claim 1 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% or at least 97% sequence identity to nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         7 . The method of  claim 1 , wherein the polypeptide is encoded by a polynucleotide comprising or consisting of SEQ ID NO: 1 or a subsequence thereof encoding a fragment having alpha-glucosidase activity. 
     
     
         8 . The method of  claim 1 , wherein the polypeptide is encoded by a polynucleotide comprising or consisting of nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         9 . The method of  claim 1 , wherein the polypeptide is encoded by the polynucleotide contained in plasmid pFD11F2 which is contained in  E. coli  NRRL B-30753. 
     
     
         10 . A method for producing a beer, comprising adding a polypeptide having alpha-glucosidase activity to a fermentation process for brewing of the beer, wherein the polypeptide having alpha-glucosidase activity is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to amino acids 21 to 960 of SEQ ID NO: 2; (b) a polypeptide that is encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) nucleotides 61 to 2880 of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising nucleotides 61 to 2880 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii); and (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity with nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         11 . The method of  claim 10 , wherein the polypeptide comprises an amino acid sequence having at least 95% or 97% sequence identity to amino acids 21 to 960 of SEQ ID NO: 2. 
     
     
         12 . The method of  claim 10 , wherein the polypeptide comprises or consists of SEQ ID NO: 2, or a fragment thereof having alpha-glucosidase activity. 
     
     
         13 . The method of  claim 10 , wherein the polypeptide comprises or consists of amino acids 21 to 960 of SEQ ID NO: 2. 
     
     
         14 . The method of  claim 10 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency or very high conditions with (i) nucleotides 61 to 2880 of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising nucleotides 61 to 2880 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii). 
     
     
         15 . The method of  claim 10 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% or at least 97% sequence identity to nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         16 . The method of  claim 10 , wherein the polypeptide is encoded by a polynucleotide comprising or consisting of SEQ ID NO: 1 or a subsequence thereof encoding a fragment having alpha-glucosidase activity. 
     
     
         17 . The method of  claim 10 , wherein the polypeptide is encoded by a polynucleotide comprising or consisting of nucleotides 61 to 2880 of SEQ ID NO: 1. 
     
     
         18 . The method of  claim 10 , wherein the polypeptide is encoded by the polynucleotide contained in plasmid pFD11F2 which is contained in  E. coli  NRRL B-30753.