Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby

Abstract

Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol) m , where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

1 . A bio-molecule detection surface, comprising:
 an oxide support material having a surface with hydroxyl and/or oxide anion groups, at least a portion of the hydroxyl and/or oxide anion groups bound to a metal reagent   
       wherein the metal reagent has a structure represented by the formula:
   Y(L-Pol) m    
 
       wherein:
 Y is a transition metal, magnesium or aluminum, 
 L is oxygen, sulfur, selenium or an amine, 
 each “Pol” group independently represents a polyethylene glycol, a substituted polyethylene glycol, a hydrocarbon, a substituted hydrocarbon, a fluorocarbon or a substituted fluorocarbon, and 
 m is an integer. 
 
     
     
         2 . The bio-molecule detection surface of  claim 1 , wherein Y is zirconium. 
     
     
         3 . The bio-molecule detection surface of  claim 2 , wherein L is oxygen, Pol is a hydrocarbon, and m is 1.