Mesenchymal stem cell and the method of use thereof

Abstract

Demyelinated axons were remyelinated in the demyelinated rat model by collecting bone marrow cells from mouse bone marrow and transplanting the mononuclear cell fraction separated from these bone marrow cells.

1 . A method for preparing a mononuclear cell fraction, comprising:
 diluting marrow cells or cord blood cells;   isolating a mononuclear cell fraction; and   selecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction,   wherein said selected cells are capable of differentiating into neural cells or glia cells.   
     
     
         2 . A method for treating neurodegenerative diseases, comprising:
 injecting an effective amount of cells capable of differentiating into neural cells or glia cells into a patient afflicted with a neurodegenerative disease,   wherein the cells are prepared by a method comprising:
 diluting marrow cells or cord blood cells; 
 isolating a mononuclear cell fraction; and 
 selecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction, and 
   wherein the injected cells treat said patient's neurodegenerative disease.   
     
     
         3 . A method for treating demyelinating diseases comprising:
 injecting an effective amount of cells capable of differentiating into neural cells or glia cells into a patient afflicted with a demyelinating disease,   wherein the cells are prepared by a method comprising:
 diluting marrow cells or cord blood cells; 
 isolating a mononuclear cell fraction; and 
 selecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction, and 
   wherein the injected cells treat said patient's demyelinating disease.   
     
     
         4 . A method for treating brain tumors comprising:
 injecting an effective amount of cells capable of differentiating into neural cells or glia cells into a patient afflicted with a brain tumor,   wherein the cells are prepared by a method comprising:
 diluting marrow cells or cord blood cells; 
 isolating a mononuclear cell fraction; and 
 selecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction, and 
   wherein the injected cells treat said patient's brain tumor.   
     
     
         5 . A method for treating epilepsy comprising:
 injecting an effective amount of cells capable of differentiating into neural cells or glia cells into a patient afflicted with epilepsy,   wherein the cells are prepared by a method comprising:
 diluting marrow cells or cord blood cells; 
 isolating a mononuclear cell fraction; and 
 selecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction, and 
   wherein the injected cells treat said patient's epilepsy.