Biomaterial and preparation method thereof

Abstract

A biomaterial prepared from a process comprising dehydrating the fish scales until the fish scales containing less than about 50% of water, and grinding the dehydrated fish scales into ground particles each having an average size of less than about 10,000 μm in diameter, wherein the ground particles contain a mixture of sponge like matrix and powder is provided. The invention also provides a biomaterial prepared from fish scales by a process comprising subjecting the fish scales to a heat treatment at a temperature of less than about 200° C.

1 . A method for preparing a tissue repair biomaterial from fish scales, comprising the step of:
 thorough cleaning of a fish scale, enough to pass Limulus Amebocyte Lysate (LAL) test; and   grinding said fish scale into a plurality of ground particles, wherein the ground particles contain a mixture of sponge made of fibrous tissue and powder.   
     
     
         2 . The method according to  claim 1 , further comprising subjecting the sponge made of fibrous tissue and powder to an extrusion performed at a temperature of less than about 200° C. for forming a flaky form. 
     
     
         3 . The method according to  claim 1 , further comprising a step of dehydrating the fish scale before grinding the fish scale, but after cleaning the fish scale. 
     
     
         4 . The method according to  claim 3 , wherein the step of dehydrating the fish scale comprises dehydrating the fish scale until the fish scale containing less than about 50% of water. 
     
     
         5 . The method according to  claim 1 , further comprising a step of separating the sponge made of fibrous tissue and powder from the ground particles. 
     
     
         6 . The method according to  claim 1 , wherein the step of grinding comprises grinding the fish scale into a plurality of ground particles until the ground particles having an average size of less than about 10,000 μm in diameter. 
     
     
         7 . The method according to  claim 1 , wherein the step of grinding comprises grinding a fish scale into a plurality of ground particles, wherein the fish scale has an average diameter of less than 20 cm. 
     
     
         8 . A method for preparing a tissue repair biomaterial from fish scales, comprising the step of:
 thorough cleaning of a fish scale, enough to pass Limulus Amebocyte Lysate (LAL) test; and   subjecting the fish scale to an extrusion process for forming a flaky form.   
     
     
         9 . The method according to  claim 8 , wherein the extrusion is performed at a temperature of less than about 200° C. 
     
     
         10 . The method according to  claim 8 , further comprising a step of dehydrating the fish scale after cleaning the fish scale, but before subjecting the fish scale to the extrusion process. 
     
     
         11 . The method according to  claim 10 , wherein the step of dehydrating the fish scale comprises dehydrating the fish scale until the fish scale containing less than about 50% of water. 
     
     
         12 . The method according to  claim 8 , further comprising a step of soaking the fish scale in water before subjecting the fish scale to the extrusion process. 
     
     
         13 . The method according to  claim 8 , wherein the step of subjecting the fish scale to the extrusion process comprises subjecting the fish scale to the extrusion process for forming the flaky form, wherein the fish scale has an average diameter of less than 20 cm.