Specific component measuring method by spectral measurement

Abstract

The present invention discloses a method for measuring an amount of an objective component to be measured in a sample, which comprises; preventing an electric charge in an atmosphere in a photometry chamber from transferring to the surface of a solution which generates light due to an energy variation of a substance induced by the objective component in the sample, measuring value of the light, and determining an amount of the objective component in the sample on the basis of the measured value thus obtained, and an instrument used for the method. According to the present invention, in measurement of an objective component in a sample using a spectrophotometer, problems such as between-day variation of signal values or increase of background value, etc. can be solved, and a trace component can be measured in high accuracy and high sensitivity.

What is claimed is: 
     
         1 . An instrument for measuring value of light generated due to an energy variation of a substance in a solution instrument, which contains a mechanism for measuring value of light generated due to an energy variation of a substance in a solution and a mechanism for preventing an electric charge in an atmosphere in a photometry chamber from transferring to the surface of the solution,
 wherein the mechanism for preventing the electric charge in the atmosphere in the photometry chamber from transferring to the surface of the solution is a mechanism for making an atmosphere surrounding a reaction vessel and/or an atmosphere surrounding the reaction vessel in the photometry chamber electrically constant, and is one or more mechanism selected from the group consisting of:   (1) an apparatus for flowing gas having a constant electric charge to the atmosphere surrounding the reaction vessel and/or into the photometry chamber,   (2) a neutralization apparatus, or   (3) a photometry chamber on which a material having a static electricity elimination effect is attached.   
     
     
         2 . The instrument according to  claim 1 , wherein the light generated due to the variation of the energy of the substance is luminescence, fluorescence, or phosphorescence, which is generated corresponding to a presence of an objective component to be measured in a sample. 
     
     
         3 . The instrument according to  claim 1 , wherein the mechanism for making the atmosphere surrounding the reaction vessel and/or the atmosphere surrounding the reaction vessel in the photometry chamber electrically constant is two or more mechanisms selected from the group consisting of:
 (1) an apparatus for flowing gas having a constant electric charge to the atmosphere surrounding the reaction vessel and/or into the photometry chamber,   (2) a neutralization apparatus, or   (3) a photometry chamber on which a material having a static electricity elimination effect is attached.