Electrophoresis gel cassette and comb

Abstract

Provided herein is an apparatus for gel electrophoresis comprising a cassette and a comb having at least one wedge-shaped tooth.

1 . An apparatus for gel electrophoresis comprising:
 a gel cassette; and   a comb having at least one wedge-shaped tooth.   
     
     
         2 . The apparatus of  claim 1 , wherein the gel cassette includes a retainer plate and a divider plate. 
     
     
         3 . The apparatus of  claim 2 , wherein the gel cassette includes a lip extending from the exterior surface of at least one of the divider plate or the retainer plate. 
     
     
         4 . The apparatus of  claim 1 , wherein wedge-shaped tooth has a volume of at least 40 uL. 
     
     
         5 . The apparatus of  claim 1 , wherein the cassette has at least one interior surface. 
     
     
         6 . The apparatus of  claim 5 , wherein the at least one interior surface is coated with a coating. 
     
     
         7 . The apparatus of  claim 6 , wherein the coating is an oxygen interference coating. 
     
     
         8 . The apparatus of  claim 7 , wherein the oxygen interference coating is selected from at least one of SiO or SiO 2 . 
     
     
         9 . An apparatus for gel electrophoresis comprising a comb having wedge-shaped teeth configured to create a sample well in a 1 mm gel, wherein the sample well size is at least 10% larger than a gel having a sample well created by a standard comb. 
     
     
         10 . The apparatus of  claim 9 , wherein the sample well size is at least 100% larger than a gel having a sample well created by a standard comb. 
     
     
         11 . The apparatus of  claim 10 , wherein the sample well created by a standard mini gel ten-tooth comb has a volume of 37.5 μL. 
     
     
         12 . An apparatus for gel electrophoresis comprising a ten-tooth mini gel comb having wedge-shaped teeth configured to create a sample well in a 1 mm gel, wherein the sample well has a volume of at least 70 μL. 
     
     
         13 . An apparatus for gel electrophoresis comprising a comb having wedge-shaped teeth configured to create sample wells configured to receive at least 10% more sample than a standard gel sample well. 
     
     
         14 . The apparatus of  claim 13 , wherein the apparatus is configured to create a gel having sharp bands. 
     
     
         15 . The apparatus of  claim 13 , wherein the apparatus is configured to create a gel having high resolution. 
     
     
         16 . The apparatus of  claim 1  comprising a recessed area on the comb. 
     
     
         17 . The apparatus of  claim 16  wherein the recessed area is configured to prevent the formation of a skin during gel formation. 
     
     
         18 . An apparatus for gel electrophoresis comprising:
 a gel cassette having a lip extending at an angle from at least one exterior surface of the cassette; and   a comb having at least one wedge-shaped tooth.   
     
     
         19 . An apparatus for gel electrophoresis comprising
 a gel cassette having a cavity; and   a comb,   wherein the comb comprises at least one tooth located on the comb, the at least one tooth has at least one side that is slanted, and the at least one tooth is located at least 0.335 inch from the edge of the cavity.