Method for measuring hypochlorite ion

Abstract

A method for measuring hypochlorite ion, which comprises the steps of: (A) reacting, with hypochlorite ion, a compound represented by the following general formula (I): wherein R 1 represents a 2-carboxyphenyl group which may be substituted; R 2 represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X 1 and X 2 each independently represents either hydrogen atom or a halogen atom; or a salt thereof; and (B) measuring fluorescence of a dearylated compound generated in the aforementioned step (A) or a salt thereof.

1 . A kit for measuring hypochlorite ion, which comprises individually packaged a compound represented by the general formula (I) or a salt thereof: 
       
         
           
           
               
               
           
         
       
       wherein R 1  represents a 2-carboxyphenyl group which may be substituted; R 2  represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X 1  and X 2  each independently represents hydrogen atom or a halogen atom; and
 a compound represented by the general formula (II) or a salt thereof: 
 
       
         
           
           
               
               
           
         
         wherein R 3  represents a 2-carboxyphenyl group which may be substituted; R 4  represents a hydroxyphenyl group; X 3  and X 4  each independently represents hydrogen atom or a halogen atom. 
       
     
     
         2 . The kit according to  claim 1  wherein R 2  is 4-aminophenyl group. 
     
     
         3 . The kit according to  claim 1  wherein R 4  is 4-hydroxyphenyl group. 
     
     
         4 . The kit according to  claim 2  wherein R 4  is 4-hydroxyphenyl group.