Card domain containing polypeptides, encoding nucleic acids, and methods of use

Abstract

The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.

1 . An isolated nucleic acid molecule encoding a CARD-containing polypeptide, or a CARD, NB-ARC, ANGIO-R, LRR or SAM domain therefrom, selected from:
 (a) DNA encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NOS: 12, 168, 188, 170, 172, 174, 176, 97, 99, 101, 103, 178, 180, 182, 184, 86 and 90; and   (b) DNA that hybridizes to the DNA of (a) under moderately stringent conditions, wherein said DNA encodes a biologically active polypeptide.   
     
     
         2 . The nucleic acid molecule of  claim 1 , wherein the nucleotide sequence of said nucleic acid molecule comprises any of SEQ ID NOS:11, 167, 187, 169, 171, 173, 175, 96, 98, 100, 102, 177, 179, 181, 183, 85 and 89. 
     
     
         3 . The nucleic acid molecule of  claim 1 , wherein said nucleic acid molecule is cDNA. 
     
     
         4 . A vector containing the nucleic acid molecule of  claim 1 . 
     
     
         5 . Recombinant cells containing the nucleic acid molecule of  claim 1 . 
     
     
         6 . An isolated oligonucleotide comprising at least 15 contiguous nucleotides of the nucleic acid molecule of  claim 2 . 
     
     
         7 . An oligonucleotide according to  claim 6 , wherein said oligonucleotide is labeled with a detectable marker. 
     
     
         8 . A kit for detecting the presence of CARD-encoding nucleic acid molecule comprising at least one oligonucleotide according to  claim 6 . 
     
     
         9 . An isolated CARD-containing polypeptide, or a CARD, NB-ARC, ANGIO-R, LRR or SAM domain therefrom, comprising an amino acid sequence at least 70% identical to the amino acid sequence set forth in any of SEQ ID NOS:12, 168, 188, 170, 172, 174, 176, 97, 99, 101, 103, 178, 180, 182, 184, 86 and 90. 
     
     
         10 . The CARD-containing polypeptide of  claim 9 , wherein said polypeptide is encoded by a nucleotide sequence set forth as any of SEQ ID NOS:11, 167, 187, 169, 171, 173, 175, 96, 98, 100, 102, 177, 179, 181, 183, 85 and 89. 
     
     
         11 . A peptide, comprising at least 10 contiguous amino acids of the polypeptide of  claim 9 . 
     
     
         12 - 26 . (canceled) 
     
     
         27 . A composition comprising a compound selected from the group consisting of a CARD-containing polypeptide, a functional fragment therefrom, and an anti-CARD antibody; and a pharmaceutically acceptable carrier. 
     
     
         28 . (canceled) 
     
     
         29 . A chimeric polypeptide comprising a domain of  claim 9 , wherein said domain is selected from the group consisting of SEQ ID NOS:168, 170, 172, 174, 176, 178, 180, 182 and 184. 
     
     
         30 . (canceled)