Novel fructosyl peptide oxidase

Abstract

The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

1 - 25 . (canceled) 
     
     
         26 . A method for producing a fructosyl peptide oxidase comprising:
 culturing in a suitable medium a filamentous fungus which belongs to  Achaetomium  for a time and under conditions suitable for production of a fructosyl peptide oxidase; and   recovering the fructosyl peptide oxidase.   
     
     
         27 . The method of  claim 26 , wherein said  Achaetomium  produces a fructosyl peptide oxidase in a medium which contains fructosyl valyl histidine. 
     
     
         28 . The method of  claim 26 , wherein said fructosyl peptide oxidase acts on fructosyl valyl histidine in the presence of oxygen and catalyzes a reaction that produces α-ketoaldehyde, valyl histidine and hydrogen peroxide. 
     
     
         29 . The method for producing a fructosyl peptide oxidase according to  claim 26 , wherein the filamentous fungus belonging to  Achaetomium  is selected from a group consisting of  Achaetomium luteum  ATCC 18524 , Achaetomium strumarium  NISL 9324 , Achaetomium globosum  NISL 9321 , Achaetomium luteum  NISL 9323 , Achaetomium strumarium  NISL 9325 , Achaetomium strumarium  NISL 9326,  Achaetomium  sp. NISL 9327, and  Achaetomium  sp. NISL 9328. 
     
     
         30 . A method for producing a fructosyl peptide oxidase comprising:
 culturing in a suitable medium a filamentous fungus which belongs to  Eupenicillium  or  Coniochaeta  that produces a fructosyl peptide oxidase for a time and under conditions suitable for producing a fructosyl peptide oxidase; and   recovering the fructosyl peptide oxidase.   
     
     
         31 . The method of  claim 30 , wherein said  Eupenicillium  or  Coniochaeta  produces a fructosyl peptide oxidase in a medium which contains fructosyl valyl histidine. 
     
     
         32 . The method of  claim 30 , wherein said fructosyl peptide oxidase acts on fructosyl valyl histidine in the presence of oxygen and catalyzes a reaction that produces α-ketoaldehyde, valyl histidine and hydrogen peroxide; and which has less activity on ε-fructosyl lysine than it does on fructosyl valyl histidine or fructosyl glycine. 
     
     
         33 . The method of  claim 30 , wherein the filamentous fungus is selected from the group consisting of  Eupenicillium terrenum  ATCC 18547 , Eupenicillium senticosum  IFO 9158 , Eupenicillium idahoense  IFO 9510 , Eupenicillium euglaucum  IFO 31729, and  Coniochaeta  sp. NISL 9330 (FERM BP-7798).