US2010331193A1PendingUtilityA1

Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

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Assignee: MAXYGEN INCPriority: Feb 17, 1994Filed: Sep 11, 2009Published: Dec 30, 2010
Est. expiryFeb 17, 2014(expired)· nominal 20-yr term from priority
Inventors:Willem P. C. StemmerAndreas Crameri
C07K 2317/565C12Q 1/6811C07K 14/545C07K 16/00C12N 15/52C07K 14/43595C12N 9/86C40B 40/02C12Y 302/01023C12Q 1/68C12N 9/2471C12N 15/1037Y10S435/876C12N 15/1027C12N 15/64C07K 2317/622C12N 15/1058
73
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Claims

Abstract

Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.

Claims

exact text as granted — not AI-modified
1 . A method for evolving a protein encoded by a DNA substrate molecule comprising:
 (a) digesting at least a first and second DNA substrate molecule, wherein the at least a first and second substrate molecules differ from each other in at least one nucleotide, with a restriction endonuclease;   (b) ligating the mixture to generate a library of recombinant DNA molecules;   (c) screening or selecting the products of (b) for a desired property; and   (d) recovering a recombinant DNA substrate molecule encoding an evolved protein.   
     
     
         2 - 273 . (canceled)

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