US2010330666A1PendingUtilityA1

Vectors and Methods for Tissue Specific Synthesis of Protein in Eggs of Transgenic Hens

Individually held — no corporate assignee on recordPriority: Jun 12, 1996Filed: May 13, 2010Published: Dec 30, 2010
Est. expiryJun 12, 2016(expired)· nominal 20-yr term from priority
A01K 2217/05C12N 15/86A01K 2227/30C12N 15/8509A01K 2267/01C12N 2740/10043A01K 2217/20A01K 67/0275
50
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Claims

Abstract

Vectors and methods are provided for introducing genetic material into cells of a chicken or other avian species. More particularly, vectors and methods are provided for transferring a transgene to an embryonic chicken cell, so as to create a transgenic hen wherein the transgene is expressed in the hen's oviduct and the transgene product is secreted in the hen's eggs and/or those of her offspring. In a preferred embodiment, the transgene product is secreted in the egg white.

Claims

exact text as granted — not AI-modified
1 . A retroviral vector containing the ovalbumin promoter as defined by positions 284-1619 of SEQ ID NO: 1. 
     
     
         2 . The retroviral vector of  claim 1 , comprising a signal sequence. 
     
     
         3 . The retroviral vector of  claim 2 , comprising a transcription termination signal. 
     
     
         4 . A modified hygromycin B phototransferase gene lacking 3′ control elements except a stop codon.

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