Anti-idiotype antibodies of the human monoclonal antibody sc-1, and their production and use

Abstract

The invention features anti-idiotype antibodies of the human monoclonal antibody SC-1, as well as methods for producing and using the anti-idiotype antibodies.

1 . A method of generating an immune response in a mammal against an anti-idiotype body expressed by the hybridoma cell line with DSMZ accession number DSM ACC2625, said method comprising immunizing said mammal with the purified anti-idiotype antibody expressed by the hybridoma cell line with DSMZ accession number DSM ACC2625. 
     
     
         2 . The method of  claim 1 , wherein said anti-idiotype antibody is humanized prior to immunizing said mammal. 
     
     
         3 . The method of  claim 1 , wherein said mammal is a non-human mammal. 
     
     
         4 . The method of  claim 1 , wherein said immunizing results in cells in said mammal expressing polypeptides that specifically bind to said anti-idiotype antibody. 
     
     
         5 . The method of  claim 4 , wherein said polypeptides are antibodies. 
     
     
         6 . The method of  claim 5 , wherein said method further comprises isolating said cells expressing said polypeptides from said mammal. 
     
     
         7 . The method of  claim 6 , wherein said method further comprises fusing said cells to myeloma cells to generate an antibody-expressing hybridoma cell. 
     
     
         8 . The method of  claim 7 , wherein said method further comprises testing whether said hybridoma cell expresses an antibody that specifically binds the anti-idiotype antibody.