Recombinant yeast and branched alcohol production method using recombinant yeast

Abstract

This invention provides a recombinant yeast that can produce branched alcohol appropriate for automotive fuel and the like and a branched alcohol production method whereby branched alcohol can be produced at low cost with the use of the recombinant yeast. A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein is provided.

1 . A recombinant yeast in which a hydroxymethyl glutaryl-CoA reductase gene has been expressed to a high degree and the ADP-ribose pyrophosphatase gene and/or the yhfR gene are introduced so as to be expressed therein. 
     
     
         2 . The recombinant yeast according to  claim 1 , wherein the ADP-ribose pyrophosphatase gene is the nudF gene of  Bacillus subtilis.    
     
     
         3 . The recombinant yeast according to  claim 1 , wherein the yhfR gene is the yhfR gene of  Bacillus subtilis.    
     
     
         4 . The recombinant yeast according to  claim 1 , wherein the hydroxymethyl glutaryl-CoA reductase gene is the HMG1 gene of  Saccharomyces cerevisiae.    
     
     
         5 . The recombinant yeast according to  claim 1 , which is a yeast having a mevalonic acid pathway. 
     
     
         6 . The recombinant yeast according to  claim 1 , which is a yeast of the genus  Saccharomyces.    
     
     
         7 . The recombinant yeast according to  claim 1 , which is  Saccharomyces cerevisiae.    
     
     
         8 . A branched alcohol production method, comprising culturing the recombinant yeast according to  claim 1  and collecting a branched alcohol from the medium. 
     
     
         9 . The branched alcohol production method according to  claim 8 , wherein the ADP-ribose pyrophosphatase gene in the recombinant yeast is the nudF gene of  Bacillus subtilis.    
     
     
         10 . The branched alcohol production method according to  claim 8 , wherein the yhfR gene in the recombinant yeast is the yhfR gene of  Bacillus subtilis.    
     
     
         11 . The branched alcohol production method according to  claim 8 , wherein the hydroxymethyl glutaryl-CoA reductase gene in the recombinant yeast is the HMG1 gene of  Saccharomyces cerevisiae.    
     
     
         12 . The branched alcohol production method according to  claim 8 , wherein the recombinant yeast is a yeast having a mevalonic acid pathway. 
     
     
         13 . The branched alcohol production method according to  claim 8 , wherein the recombinant yeast is a yeast of the genus  Saccharomyces.    
     
     
         14 . The branched alcohol production method according to  claim 8 , wherein the recombinant yeast is  Saccharomyces cerevisiae.    
     
     
         15 . The branched alcohol production method according to  claim 8 , wherein 2-methyl-1-butanol and 3-methyl-1-butanol is collected as the branched alcohol.