T-cell vaccination in the treatment of hiv infection

Abstract

A method for the preparation of a T cell vaccine for the treatment of immunodeficient HIV-infected patients is described herein, based on the enrichment of autologous CD4-reactive CD8 T cells. Also described is a protocol for the implementation of T cell vaccination in immunodeficient HIV-infected, as well as a method of treatment, based on the T cell vaccine developed herein. Finally, kits for preparing the T cell vaccine as well as for implementing the protocol are also provided.

1 - 6 . (canceled) 
     
     
         7 . A kit for preparing a T cell vaccine against anti-CD4 autoimmune T cells, wherein said vaccine is to be used as therapy in subjects suffering from HIV infection-induced immunodeficiency, and wherein said kit comprises:
 a) means for obtaining peripheral blood mononuclear cells (PBMC);   b) means for cultivating PBMC;   c) stimulating factor, preferably PHA;   d) IL-2;   e) irradiated CD4-pulsed antigen presenting cells;   f) a fixating agent, preferably glutaraldehyde;   g) a neutralizing agent, preferably glycine;   h) injection men-grade saline;   i) manual with instructions on how to prepare the T cell vaccine.   
     
     
         8 . A kit for implementing a protocol for T cell vaccination of immunodeficient HIV-infected patients, said kit comprising the tools necessary for preparing a T cell vaccine against anti-CD4 autoimmune T cells, as follows:
 a) means for obtaining peripheral blood mononuclear cells (PBMC);   b) means for cultivating PMBC;   c) stimulating factor, preferably PHA;   d) IL-2;   e) irradiated CD4-pulsed antigen presenting cells;   f) a fixating agent, preferably glutaraldehyde;   g) a neutralizing agent, preferably glycine;   h) injection Injection grade saline;   g) manual with instructions on how to prepare the T cell vaccine;   
       and further comprising instructions for implementing said protocol.