Ribonucleases in which an amino acid sequence of a known ribonuclease is preceded by at least one residue at the n-terminus, and methods of making them recombinantly

Abstract

New RNases, and methods of making them, are disclosed. Each includes an amino acid sequence that is disclosed in U.S. Pat. No. 6,239,257 B1, but that is preceded by a different N-terminus residue or leader sequence.

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         20 . An isolated protein having the amino acid sequence of SEQ ID NO:60. 
     
     
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         22 . A gene that when expressed in a host encodes the protein of  claim 20 . 
     
     
         23 . A gene that when expressed in a host encodes the protein of  claim 21 . 
     
     
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         42 . An isolated protein having the amino acid sequence of SEQ ID NO:17 preceded at its N-terminal end by a leader sequence, the leader sequence having a) the first residue of SEQ ID NO:64, or b) all residues of SEQ ID NO:64, or c) any fragment of SEQ ID NO:64 that comprises the first residue of SEQ ID NO:64, said isolated protein being active against A-253 cells. 
     
     
         43 . An isolated protein having the amino acid sequence of SEQ ID NO:17 preceded at its N-terminal end by a leader sequence, the leader sequence having a) the first residue of SEQ ID NO:66, or b) all residues of SEQ ID NO:66, or c) any fragment of SEQ ID NO:66 that comprises the first residue of SEQ ID NO:66, said isolated protein being active against A-253 cells. 
     
     
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         57 . An isolated protein having the amino acid sequence of SEQ ID NO:17 preceded at its N-terminal end by a leader sequence, the leader sequence having at least the first and at most all of the residues of SEQ ID NO:62 in order.