Method for determination of presence or absence of peptide compound pyy3-36

Abstract

The present invention provides a method for determining in a pharmaceutical test formulation the presence or absence of a peptide compound PYY 3-36 represented by the following amino acid sequence: H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X (SEQ ID NO: 1), wherein X is OH or a carboxy acid-protecting group, the method comprising (1) preparing a solution by mixing the pharmaceutical test formulation with cucurbit[7]uril in a solvent; and (2) thermally analyzing the solution prepared in Step (1).

1 . A method for determining in a pharmaceutical test formulation the presence or absence of a peptide compound PYY 3-36  represented by the following amino acid sequence:
   H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X   (SEQ ID NO: 1),   
       wherein X is OH or a carboxy acid-protecting group, 
       the method comprising:
 (1) preparing a solution by mixing the pharmaceutical test formulation with cucurbit[7]uril in a solvent; and 
 (2) thermally analyzing the solution prepared in Step (1). 
 
     
     
         2 . The determination method according to  claim 1 , wherein the presence or absence of a peptide compound PYY 1-36  represented by the following amino acid sequence:
   H-Tyr-[[Pre]]Pro-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH 2    (SEQ ID NO: 2),   
       and/or peptide digests generated by degradation of the peptide compound PYY 1-36  is determined. 
     
     
         3 . The determination method according to  claim 1 , wherein peptide digests generated by degradation of the peptide compound PYY 3-36  is detected.