US2010279400A1PendingUtilityA1

Diagnostic test for west nile virus

54
Individually held — no corporate assignee on recordPriority: Oct 31, 2002Filed: Apr 1, 2008Published: Nov 4, 2010
Est. expiryOct 31, 2022(expired)· nominal 20-yr term from priority
Inventors:Susan J. WongPei-Yong Shi
G01N 33/56983C12Q 1/04Y02A50/30
54
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Claims

Abstract

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result. The invention also provides monoclonal antibodies against WNV NS5 and DENV NS5 antigen and their use in detecting WNV and DENV infections in a biological sample.

Claims

exact text as granted — not AI-modified
1 . An isolated monoclonal antibody, or antigen binding fragment thereof, wherein the monoclonal antibody is specific for WNV NS5 and not detectably cross-reactive with NS5 of a flavivirus other than WNV. 
     
     
         2 . A hybridoma cell line which produces a monoclonal antibody wherein the monoclonal antibody is specific for WNV NS5 and not detectably cross-reactive with NS5 of a flavivirus other than WNV. 
     
     
         3 - 70 . (canceled) 
     
     
         71 . The monoclonal antibody according to  claim 1  wherein the antibody is humanized. 
     
     
         72 . The monoclonal antibody according to  claim 1  wherein the antibody is equinized. 
     
     
         73 - 92 . (canceled)

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