Ps-spcl searching apparatus and method using surface plasmon resonance

Abstract

A Positional Scanning—Synthetic Peptide Combinatorial Library (PS-SPC) searching apparatus and method using Surface Plasmon Resonance (SPR) are provided. The method includes spotting and fixing each of a plurality of peptide pools to a top of one thin metal film, inputting specific materials to the top of the thin metal film, applying a TM-mode light to a bottom of the thin metal film and exciting SPR for the thin metal film, and detecting a TM mode reflected light reflected from the thin metal film and displaying the detected light as a two-dimensional image.

1 . A Positional Scanning—Synthetic Peptide Combinatorial Library (PS-SPCL) searching method using Surface Plasmon Resonance (SPR), the method comprising:
 spotting and fixing each of a plurality of peptide pools to a top of one thin metal film;   inputting specific materials to the top of the thin metal film;   applying a Transverse Magnetic (TM) mode light to a bottom of the thin metal film and exciting SPR for the thin metal film; and   detecting a TM mode reflected light reflected from the thin metal film and displaying the detected light as a two-dimensional image.   
     
     
         2 . The method of  claim 1 , wherein in spotting and fixing each of the plurality of peptide pools to the top of the thin metal film, the plurality of peptide pools are fixed to the surface of the thin metal by a covalent bond. 
     
     
         3 . The method of  claim 1 , wherein the thin metal film is a metal emitting electron by an external stimulus and having a negative dielectric constant. 
     
     
         4 . The method of  claim 2 , further comprising: before spotting and fixing each of the plurality of peptide pools to the top of the thin metal film, synthesizing the plurality of peptide pools so that peptides come in contact with the surface of the thin metal film while forming a covalent bond by manipulating N-terminals or C-terminals of the peptides. 
     
     
         5 . The method of  claim 4 , wherein in synthesizing the plurality of peptide pools, the functional group consisting of a hydrogen atom (—SH) is added to the N-terminals or C-terminals. 
     
     
         6 . The method of  claim 4 , wherein in detecting the TM-mode reflected light reflected from the thin metal film and displaying the detected light as the two-dimensional image, brightness of each point on a two-dimensional plane is detected through a Charge-Coupled Device (CCD) or a Complementary Metal Oxide Semiconductor (CMOS) image sensor. 
     
     
         7 . The method of  claim 1 , further comprising:
 detecting optimal amino acids by position from the two-dimensional image.   
     
     
         8 . The method of  claim 7 , wherein in detecting the optimal amino acids by position, the optimal amino acids of the position are detected as amino acids that are fixed to peptide pools appearing most bright on the two-dimensional image among a peptide pool group whose different amino acids are fixed in the same position. 
     
     
         9 . The method of  claim 1 , further comprising: after displaying as the two-dimensional image,
 removing the target materials inputted to the top of the thin metal film by a physical or chemical method.   
     
     
         10 . The method of  claim 9 , wherein in removing the target materials by the physical or chemical method, the target materials are removed using a chaotropic reagent such as urea, guanidine hydrochloride or a detergent such as sodium dodecyl sulfate. 
     
     
         11 . A Positional Scanning—Synthetic Peptide Combinatorial Library (PS-SPCL) searching apparatus using Surface Plasmon Resonance (SPR), the apparatus comprising:
 a PS-SPCL chip comprising:   a thin metal film for spotting and fixing one or more peptide pools; and   a flat type transparent dielectric substrate for supporting the thin metal film;   an incident light provision unit for providing a TM mode incident light for exciting SPR;   a prism for propagating the incident light, which is provided by the incident light provision unit, from a bottom of the PS-SPCL chip to an interface between the thin metal film and the flat type transparent dielectric substrate and emitting a reflected light reflected from the thin metal film; and   a reflected light detector for detecting a reflected light emitted through the prism and displaying the detected light as a two-dimensional image.   
     
     
         12 . The apparatus of  claim 11 , wherein the thin metal film is a metal emitting electron by an external stimulus and having a negative dielectric constant. 
     
     
         13 . The apparatus of  claim 11 , wherein the flat type transparent dielectric substrate is formed of high refractive-index polymer materials comprising slide glass or CycloOlefin Copolymer (COC). 
     
     
         14 . The apparatus of  claim 11 , wherein the plurality of peptide pools are coupled by a covalent bond with a surface of the thin metal film by manipulating N-terminals or C-terminals. 
     
     
         15 . The apparatus of  claim 11 , wherein the reflected light detector comprises a Charge-Coupled Device (CCD) or a Complementary Metal Oxide Semi-conductor (CMOS) image sensor. 
     
     
         16 . The apparatus of  claim 11 , further comprising:
 a signal processor for analyzing the two-dimensional image displayed by the reflected light detector and detecting optimal amino acids by position as amino acids that are fixed to peptide pools appearing most bright on the two-dimensional image among a peptide pool group whose different amino acids are fixed in the same position.