US2010260729A1PendingUtilityA1

Corn Event PV-ZMIR13 (MON863) Plants and Compositions and Methods for Detection Thereof

41
Individually held — no corporate assignee on recordPriority: Jul 29, 2002Filed: Apr 26, 2010Published: Oct 14, 2010
Est. expiryJul 29, 2022(expired)· nominal 20-yr term from priority
Inventors:Tracey A. CavatoTimothy R. CoombeScott C. Johnson
C07K 14/415C12N 15/8271C12N 15/8286Y02A40/146C12Q 1/6895
41
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The present invention provides compositions and methods for detecting the presence of the corn event MON863 DNA inserted into the corn genome from the transformation of the recombinant construct containing a Cry3Bb gene and of genomic sequences flanking the insertion site. The present invention also provides the corn event MON863 plants, progeny and seeds thereof that contain the corn event MON863 DNA.

Claims

exact text as granted — not AI-modified
1 - 42 . (canceled) 
     
     
         43 . At least one DNA molecule of sufficient length of contiguous nucleotides homologous or complementary to SEQ ID NO:3 or SEQ ID NO:4 to function as a DNA primer or probe specific for corn event MON863 or progeny thereof. 
     
     
         44 . The at least one DNA molecule of  claim 43 , comprising a nucleotide sequence of SEQ ID NO:1, SEQ ID NO:2, or the complement thereof. 
     
     
         45 . The at least one DNA molecule of  claim 43 , comprising a nucleotide sequence of SEQ ID NO:3, SEQ ID NO:4, or the complement thereof. 
     
     
         46 . The at least one DNA molecule of  claim 43 , comprising a pair of DNA molecules, said pair comprising a first DNA molecule of 11 or more contiguous nucleotides of any portion of the transgene region of SEQ ID NO:3 or the complement thereof, and a second DNA molecule of a similar length of a 5′ flanking corn genomic DNA region of SEQ ID NO:3 or the complement thereof. 
     
     
         47 . The at least one DNA molecule of  claim 46 , wherein said first DNA molecule comprises 11 or more contiguous nucleotides of the 5′ transgene portion of the DNA sequence of SEQ ID NO:7 or complement thereof, and said second DNA molecule comprises a similar length of 5′ flanking corn DNA sequence of SEQ ID NO:5 or complement thereof. 
     
     
         48 . The at least one DNA molecule of  claim 47 , wherein said first DNA molecule comprises a sequence that is homologous or complementary to SEQ ID NO:7, and said second DNA molecule comprises a similar length of a sequence that is homologous or complementary to SEQ ID NO:5. 
     
     
         49 . The at least one DNA molecule of  claim 46 , wherein said first DNA molecule comprises SEQ ID NO:10 and said second DNA molecule comprises SEQ ID NO:9. 
     
     
         50 . The at least one DNA molecule of  claim 43 , comprising a pair of DNA molecules, said pair comprising a first DNA molecule of 11 or more contiguous nucleotides of any portion of the transgene region of SEQ ID NO:4 or the complement thereof, and a second DNA molecule of a similar length of a 3′ flanking corn genomic DNA region of SEQ ID NO:4 or the complement thereof. 
     
     
         51 . The at least one DNA molecule of  claim 50 , wherein said first DNA molecule comprises 11 or more contiguous nucleotides of the 3′ transgene portion of the DNA sequence of SEQ ID NO:8, or the complement thereof, and said second DNA molecule comprises a similar length of 3′ flanking corn DNA of SEQ ID NO:6, or the complement thereof. 
     
     
         52 . The at least one DNA molecule of  claim 51 , wherein said first DNA molecule comprises a sequence that is homologous or complementary to SEQ ID NO:8, and said second DNA molecule comprises a similar length of a sequence that is homologous or complementary to SEQ ID NO:6. 
     
     
         53 . The at least one DNA molecule of  claim 50 , wherein said first DNA molecule comprises SEQ ID NO:11 and said second DNA molecule comprises SEQ ID NO:12. 
     
     
         54 . A method of detecting the presence of a DNA molecule selected from the group consisting of SEQ ID NO:3 and SEQ ID NO:4 in a biological sample, the method comprising:
 (a) contacting said biological sample with at least one DNA molecule according to any one of  claims 46 - 53 ;   (b) providing a nucleic acid amplification reaction condition;   (c) performing said nucleic acid amplification reaction, thereby producing a DNA amplicon molecule; and   (d) detecting said DNA amplicon molecule, wherein detection of an amplicon comprising at least one of SEQ ID NO:1, SEQ ID NO:2 and the complement thereof is indicative of the presence of a DNA molecule selected from the group consisting of SEQ ID NO:3 and SEQ ID NO:4 in said biological sample.   
     
     
         55 . The method of  claim 54 , wherein said biological sample is a DNA sample extracted from a corn plant and is selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products. 
     
     
         56 . A method of detecting the presence of a DNA molecule selected from the group consisting of SEQ ID NO:3 and SEQ ID NO:4 in a biological sample, the method comprising:
 (a) contacting said biological sample with at least one DNA molecule according to any one of  claims 43 - 45 ;   (b) subjecting said biological sample and at least one DNA molecule to stringent hybridization conditions; and   (c) detecting hybridization of said at least one DNA molecule to said biological sample, wherein detection of hybridization is indicative of the presence of a DNA molecule selected from the group consisting of SEQ ID NO:3 and SEQ ID NO:4 in said biological sample.   
     
     
         57 . The method of  claim 56 , wherein said biological sample is a DNA sample extracted from a corn plant and is selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products. 
     
     
         58 . A DNA detection kit, comprising at least one DNA molecule according to any one of  claims 43  to  53 . 
     
     
         59 . A method of determining zygosity of DNA of a corn plant comprising corn event MON863 in a biological sample comprising:
 (a) contacting said sample with at least one DNA molecule according to any one of  claims 46  to  53 , said at least one DNA molecule is a primer set comprising SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:12, or a primer set comprising SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, that (1) when used in a nucleic acid amplification reaction comprising corn event MON863 DNA, produces a first amplicon that is diagnostic for corn event MON863, and (2) when used in a nucleic acid amplification reaction comprising corn genomic DNA other than MON863 DNA, produces a second amplicon that is diagnostic for corn genomic DNA other than MON863 DNA;   (b) performing a nucleic acid amplification reaction; and   (c) detecting the amplicons so produced, wherein detection of presence of both amplicons indicates that said sample is heterozygous for corn event MON863 DNA, wherein detection of only the first amplicon indicates that said sample is homozygous for corn event MON863 DNA.   
     
     
         60 . A transgenic corn plant cell comprising DNA encoding a Cry3Bb and DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2. 
     
     
         61 . An insect resistant corn plant, or parts thereof, comprising the transgenic corn plant cell of  claim 60 , wherein said plant, or parts thereof, comprises said DNA encoding a Cry3Bb and said DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2. 
     
     
         62 . Seed of the insect resistant corn plant of  claim 61 , wherein said seed comprises said DNA encoding a Cry3Bb and said DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2. 
     
     
         63 . Seed of corn plant designated MON863, having representative sample deposited with the American Type Culture Collection (ATCC) under accession number PTA-2605. 
     
     
         64 . A corn plant MON863 or parts thereof produced by growing the seed of  claim 63 . 
     
     
         65 . A corn plant, seed, or parts thereof, comprising corn event MON863. 
     
     
         66 . A corn plant, seed, or parts thereof, capable of producing a MON863 diagnostic amplicon. 
     
     
         67 . The corn plant, seed, or parts thereof, of  claim 66 , wherein said MON863 diagnostic amplicon comprises SEQ ID NO:1 or SEQ ID NO:2. 
     
     
         68 . A composition derived from the transgenic corn plant, or parts thereof, as set forth in  claim 61 , wherein said composition comprises a detectable amount of said DNA encoding a Cry3Bb and said DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, and wherein said composition is a commodity product selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, popcorn, corn cakes, cereals containing corn and corn by-products. 
     
     
         69 . A method of producing an insect resistant corn plant comprising:
 (a) sexually crossing a first insect resistant corn plant MON863 having representative seed deposited with the American Type Culture Collection (ATCC) under accession number PTA-2605 and a second parent corn plant that lacks insect resistance, thereby producing a plurality of progeny plants; and   (b) selecting a progeny plant that is insect resistant.   
     
     
         70 . The method of  claim 69 , further comprising backcrossing the progeny plant that is insect resistant to the second parent corn plant, thereby producing a plant that is insect resistant. 
     
     
         71 . A method of producing an insect resistant corn plant, comprising:
 (a) crossing the corn plant of any one of  claims 61  and  64  to  67  with another corn plant; and   (b) selecting a progeny plant that is insect resistant by analyzing for the presence of at least one nucleotide sequence of SEQ ID NO:1 and SEQ ID NO:2.   
     
     
         72 . The method of  claim 71 , wherein said selecting step (b) includes subjecting the progeny plant to a nucleic acid amplification reaction, wherein progeny plant that produces an amplicon comprising at least one nucleotide sequence of SEQ ID NO:1 and SEQ ID NO:2 is selected, or subjecting the progeny plant to a nucleic acid hybridization reaction, wherein progeny hybridizing to a probe that hybridizes under stringent conditions with one or more DNA sequence selected from SEQ ID NO:1 and SEQ ID NO:2 is selected. 
     
     
         73 . A method of producing an insect resistant corn plant, comprising
 (a) transforming a corn plant cell with DNA encoding a Cry3Bb and DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2; and   (b) regenerating a corn plant from said transformed cell, wherein said corn plant comprises said DNA encoding a Cry3Bb and said DNA having nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2 and is insect resistant.   
     
     
         74 . A method for protecting a corn plant from insect infestation, comprising providing in the diet of a Coleopteran pest of corn an insecticidally effective amount of cell(s) or tissue(s) of the corn plant, or parts thereof, of any one of  claims 61  and  64  to  67 . 
     
     
         75 . The method of  claim 74 , wherein said Coleopteran pest is selected from the group consisting of  Diabrotica vergifera, Diabrotica undecimpunctata , and  Leptinotarsa decemlineata.

Join the waitlist — get patent alerts

Track US2010260729A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.