Paramyxoviridae Virus Preparations

Abstract

This document involves methods and materials related to obtaining Paramyxoviridae virus preparations.

1 . A composition comprising measles virus, wherein said composition is sterile, has a volume greater than 500 mL, and has a measles virus titer greater than 10 6  TCID 50  per mL, wherein said composition comprises less than 750 μg total protein per mL and less than 1 μg of host cell DNA per mL, wherein said composition is free of bovine serum albumin, and wherein the maximum length of the non-viral DNA of said composition is less than 500 bp. 
   
   
       2 . The composition of  claim 1 , wherein said measles virus comprises a nucleic acid sequence that encodes a polypeptide, wherein said polypeptide is heterologous to said virus. 
   
   
       3 . The composition of  claim 2 , wherein said polypeptide comprises an amino acid sequence present in human carcinoembryonic antigen, said amino acid sequence being at least ten amino acid residues in length. 
   
   
       4 . The composition of  claim 1 , wherein the measles virus titer is between 10 9  TCID 50  per mL and 10 15  TCID 50  per mL. 
   
   
       5 . The composition of  claim 1 , wherein the measles virus titer is between 10 12  TCID 50  per mL and 10 15  TCID 50  per mL. 
   
   
       6 . The composition of  claim 1 , wherein said composition comprises less than 250 μg total protein per mL. 
   
   
       7 . The composition of  claim 1 , wherein the maximum length of the non-viral DNA of said composition is less than 200 bp.