US2010234450A1PendingUtilityA1
Molecular targeting agents
Individually held — no corporate assignee on recordPriority: Aug 9, 2008Filed: Mar 16, 2010Published: Sep 16, 2010
Est. expiryAug 9, 2028(~2 yrs left)· nominal 20-yr term from priority
C12N 2310/3519A61K 47/549C12N 15/115C12N 2320/32C12N 2310/322C12N 15/1135C12N 2310/16A61K 47/554A61P 35/00C12N 2310/344C12N 2310/14A61K 31/7088
44
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Claims
Abstract
The present invention relates to aptamer conjugates comprising a metal chelating group and to methods of using these aptamers.
Claims
exact text as granted — not AI-modified1 . A conjugate comprising a nucleic acid aptamer that is not more than 45 nucleotides in length comprising the nucleic acid sequence 5′-n 1 n 2 n 3 CGGAUCAGCn 4 n 5 n 6 GUUUA-3′ (SEQ ID NO:1), linked to one or more chelating groups, wherein each n x can be present or absent, wherein when present each n x represents any nucleotide.
2 . The conjugate of claim 1 , wherein nucleotides n 1 n 2 n 3 and n 4 n 5 n 6 are present and hybridize to form a stem structure.
3 . The conjugate of claim 1 , wherein the nucleic acid aptamer molecule comprises the nucleic acid sequence 5′-AUGCGGAUCAGCCAUGUUUA-3′ (SEQ ID NO:2).
4 . The conjugate of claim 1 , wherein the nucleic acid aptamer molecule comprises the nucleic acid sequence 5′-n a n b n c n d n 1 n 2 n 3 CGGAUCAGCn 4 n 5 n 6 GUUUAn e n f n g n h -3′ (SEQ ID NO:3).
5 . The conjugate of claim 4 , wherein nucleotides n 1 n 2 n 3 and n 4 n 5 n 6 are present and hybridize to form a first stem structure and nucleotides n a n b n c n d and n e n f n g n h are present and hybridize to form a second stem structure.
6 . The conjugate of claim 1 , wherein the nucleic acid aptamer molecule comprises the nucleic acid sequence 5′-GACGAUGCGGAUCAGCCAUGUUUACGUC-3′ (SEQ ID NO:4).
7 . The conjugate of claim 1 , wherein the nucleic acid aptamer molecule comprises the nucleic acid sequence 5′-n 10 n 11 n 12 n 13 n 14 n a n b n c n d n 1 n 2 n 3 CGGAUCAGCn 4 n 5 n 6 GUUUAn e n f n g n h n 15 n 16 n 17 n 18 n 19 n 20 n 21 -3′ (SEQ ID NO:6).
8 . The conjugate of claim 7 , wherein nucleotides n 1 n 2 n 3 and n 4 n 5 n 6 are present and hybridize to form a first stem structure, nucleotides n a n b n c n d and n e n f n g n h are present and hybridize to form a second stem structure, and nucleotides n 10 n 11 n 12 n 13 n 14 and n 16 n 17 n 18 n 19 n 20 are present and hybridize to form a third stem structure.
9 . The conjugate of claim 1 , wherein the nucleic acid aptamer molecule comprises the nucleic acid sequence aptamer A10-3.2 (5′-GGGAGGACGAUGCGGAUCAGCCAUGUUUACGUCACUCCU-3′ (SEQ ID NO:5)).
10 . The conjugate of claim 1 , wherein a linker links the aptamer to the one or more chelating groups.
11 . A pharmaceutical composition comprising the conjugate of claim 1 and a pharmaceutically acceptable carrier.Join the waitlist — get patent alerts
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