Novel receptor that binds ctla-8

Abstract

Isolated receptors for CTLA-8, DNAs encoding such receptors, and pharmaceutical compositions made therefrom, are disclosed. The isolated receptors can be used to inhibit an immune response.

1 . An isolated DNA selected from the group consisting of:
 (a) a DNA having a nucleotide sequence encoding an amino acid sequence of amino acids 1 through 322 of SEQ ID NO.: 2; and   (b) DNA molecules capable of hybridization to the clones of (a) under stringent conditions and which encode CTLA-8R capable of binding CTLA-8.   
     
     
         2 . An isolated DNA according to  claim 1  which encodes a soluble CTLA-8R. 
     
     
         3 . An isolated DNA selected from the group consisting of:
 (a) a DNA having a nucleotide sequence encoding an amino acid sequence of amino acids 1 through 322 of SEQ ID NO.: 2; and   (b) DNA molecules encoding peptides that are at least about 70% identical in amino acid sequence to the amino acid sequence of SEQ ID NO:2.   
     
     
         4 . A recombinant expression vector comprising a DNA sequence according to  claim 1 . 
     
     
         5 . A recombinant expression vector comprising a DNA sequence according to  claim 2 . 
     
     
         6 . A recombinant expression vector comprising a DNA sequence according to  claim 3 . 
     
     
         7 . A host cell transformed or transfected with an expression vector according to  claim 4 . 
     
     
         8 . A host cell transformed or transfected with an expression vector according to  claim 5 . 
     
     
         9 . A host cell transformed or transfected with an expression vector according to  claim 6 . 
     
     
         10 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to  claim 7  under conditions promoting expression. 
     
     
         11 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to  claim 8  under conditions promoting expression. 
     
     
         12 . A process for preparing a CTLA-8R or an analog thereof, comprising culturing a host cell according to  claim 9  under conditions promoting expression. 
     
     
         13 . An isolated and purified biologically active CTLA-8 receptor (CTLA-8R) protein. 
     
     
         14 . An isolated and purified CTLA-8R protein according to  claim 13 , consisting essentially of human CTLA-8R. 
     
     
         15 . An isolated and purified CTLA-8R according to  claim 13 , consisting essentially of soluble CTLA-8R. 
     
     
         16 . A composition comprising a CTLA-8R protein according to  claim 13 , and a suitable diluent or carrier. 
     
     
         17 . A method for regulating an immune or inflammatory response in a mammal, comprising administering an effective amount of a composition according to  claim 16 . 
     
     
         18 . An assay method for detection of CTLA-8 and homologs thereof, or CTLA-8R and homologs thereof, or the interaction thereof, comprising use of a protein composition according to  claim 16 . 
     
     
         19 . Antibodies immunoreactive with CTLA-8R.