Preparation method of a hair dermal papilla cell preparation, composition and method for regenerating hair follicles, and animal having regenerated hair follicles

Abstract

The present invention provides a method for preparing a hair dermal papilla cell preparation comprising preparing a cell suspension by removing epidermal tissue from skin tissue and subjecting the resulting dermal tissue fraction to collagenase treatment, and cyropreserving the cell suspension to kill the follicular epidermal cells. The present invention also provides a composition for regenerating hair follicles comprising hair dermal papilla cell and epidermal cells, wherein the ratio of the number of hair dermal papilla cell to the number of epidermal cells is from 1:10 to 10:1.

1 . A method for preparing a hair dermal papilla cell preparation comprising: providing a cell suspension by removing epidermal tissue from skin tissue and subjecting the resulting dermal tissue fraction to collagenase treatment, and cryopreserving the cell suspension to kill the follicular epidermal cells. 
     
     
         2 . A method according to  claim 1 , wherein the cryopreservation is carried out after adjusting the cell density of the cell suspension to 1×10 5  to 1×10 8 /ml. 
     
     
         3 . A method according to  claim 1 , wherein the cryopreservation is carried out at a temperature of −80° C. or lower. 
     
     
         4 . A method according to  claim 1 , wherein the cryopreservation is carried out in liquid nitrogen. 
     
     
         5 . A method according to  claim 1 , wherein the cryopreservation is carried out for a period of 1 week or more. 
     
     
         6 . A method according to  claim 1 , wherein the skin tissue is from a mouse. 
     
     
         7 . A method according to  claim 1 , wherein the skin tissue is from a rat. 
     
     
         8 . A method according to  claim 1 , wherein the skin tissue is from a human. 
     
     
         9 - 36 . (canceled)