Methods for the specific preparation of lysobactin fragments

Abstract

The invention relates to methods for the targeted production of lysobactin derivatives by combined chemical and enzymatic modifications. In particular, the invention relates to method for preparing lysobactin fragment 4-11 by chemical reduction and cleavage of the resultant product by chymotrypsin.

1 . A method for the synthesis of lysobactin derivatives using lysobactin fragment 4-11. 
   
   
       2 . A method for preparing open-chain lysobactin derivatives, whereby lysobactin fragment 4-11 is reacted with a tripeptide having a C-terminal aromatic or hydrophobic amino acid in a buffer medium with addition of a C1 -4 -alcohol, whereby the tripeptide is present in the form of the free acid or an ester and whereby the concentration of the C1 -4 -alcohol in the reaction medium is greater than 40%. 
   
   
       3 . The method of  claim 2 , whereby methanol is used as C1 -4 -alcohol.