US2010216212A1PendingUtilityA1
Anti-obesity agent and anti-obesity food
Assignee: SCHOOL CORP AZABU VETERIN MEDPriority: Jul 26, 2005Filed: Jul 25, 2006Published: Aug 26, 2010
Est. expiryJul 26, 2025(expired)· nominal 20-yr term from priority
A61P 3/04C12N 9/0006C12N 9/90A61K 35/747C07K 14/335C12N 9/20A61K 48/00C12N 9/0008A23V 2002/00C12N 9/1205A23L 33/135A61K 38/46A61K 35/74C12R 2001/225C12N 1/205A23V 2400/173
53
PatentIndex Score
0
Cited by
0
References
0
Claims
Abstract
An anti-obesity agent containing, as an active ingredient, a microorganism which belongs to the species Lactobacillus reuteri and is capable of producing lipases having the amino acid sequences respectively depicted in SEQ ID NO: 1, 3 or 5 or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences respectively depicted in SEQ ID NO: 1, 3 or 5. The anti-obesity agent enables a patient to take a normal meal yet preventing the absorption of a fat into the body which is the primary cause of obesity.
Claims
exact text as granted — not AI-modified1 . An anti-obesity agent comprising, as an active ingredient, a microorganism belonging to the species Lactobacillus reuteri and capable of producing lipases depicted in the following amino acid sequences (1) to (3) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (1) to (3):
(1)
MVKLMTIHELANNPTLSGQVRLIENIVYGA
MDGEALHMSILAPWTQRFPKQYQTEPRPLI
VFVQGSSWRTPKMGEEIPQLVQFVRAGYIV
ATVQHRSSIDSHPFPAFLQDVKTAIRFLRA
NAQKYAIDPQQVAIWGTSSGANAAMLVGLT
GDDPRYKVDLYQDESDAVDAVVSCFAPMDV
EKTFEYDANVPGNKLLQYCLLGPDVSKWPE
IEKQMSPLYQVKDGQNYPPFLLFHGDADKV
VPYEQMEKMYMRLKDNGNSVEAYRVKGANH
ERDFWSPTIYNIVQKFLGDQFK
(2)
LIYVLKDLCNTIAEVYGKSILKGVFIMKHT
LKVDQVRDGLWLDSDITYTQVPGWLGNTTR
DLKLSVIRHFQTNDDTRYPVIFWFAGGGWM
DTDHNVHLPNLVDFARHGYIVVGVEYRDSN
KVQFPGQLEDAKAAIRYMRANAKRFQADPN
RFIVMGESAGGHMASMLGVTNGLNQFDKGA
NLDYSSDVQVAVPFYGVVDPLTAKTGSASN
DFDFVYRNLLGAEPENAPELDSAANPLTYV
NSNSTPFLIFHGTEDVVVPIKDSEKLYDAL
VENNVPAELYEIEGASHMDVKFLQPQVFKI
VMDFLDKYLTRS
(3)
MEIKSVNLDQPYSSLDIYHSNTDKALPGLV
ILPGGSYNQIMERDSERVALTFATHAWQTF
VVRYPVVEHKNYEEAKIAVHQAFEYIVNHA
AELDVDADRLGIIGFSAGGQIAAAYSNEKL
THARFAALGYPVIQPLIDERMGVTTENVAK
LVNPQTPPTFMWGSAKDELTPFVDHLQVYA
DALIKNDIPYELHEFGTGGHGIALANEYTG
IVNNDRVDNHMGKWFPLFLEWLTELNLI
2 . The anti-obesity agent according to claim 1 , wherein the microorganism belonging to the species Lactobacillus reuteri has nucleotide sequences encoding the amino acid sequences (1) to (3) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (1) to (3), and a nucleotide sequence depicted in the following formula (4):
(4)
ATGCATGGATTTATTGGCGAATTTTTTGGCACCATGGTTTTAATCCTATTAGGAGCAGGA
TGTTGTGCTGGTAATAGTTTGAATAAAACATATGGGAAACAAAGTGGCTGGTGGTTTATC
TGTATTTCATGGGGCTTAGCAGTTACAATGGGAGTTTATGTTGCAGGATTTCTGGGTTCA
TTAGGGCACTTAAATCCCGCTGTAACAATTCCTTTTGCTATTTTTGGCTTATTCCCATGG
AGTAACGTTATACGTTACTTACTTGGTCAATTTCTTGGTGCGTTTGTTGGTGCAGTATTA
GTAATTATTCAATTCTATCCACAATTTAAAGCAACCCCAAATGAAGAAGAAGGAAATAAT
GTTGGTATTTTTGCTACTCGTCCAGCGATAAATAGTCCAATTTTTAACTTTTTCTCAGAA
GTGATTGCGACCTTTGCATTTATTTTCATCTTATTAAATCTTGGCAACTTTACACAGGGA
TTGAAGCCATTTATCGTAGGAATGGTTATTGCAGTTGTTGGTACATGTCTCGGGACAACT
ACTGGCTTTGCATTAAACCCAGCTCGTGATTGGTCACCACGTTTAGCATATACTATTTTG
CCAATTCCTAATAAGGGTGTTTCAGAATGGTGGTATGCATGGGTTCCAATGTGTGGCCCA
ATTGTTGGGGGCCTTCTTGCTTGTGCTTTACAAACGGCACTAGTTTAG
3 . The anti-obesity agent according to claim 1 or 2 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme composed of subunits depicted in the following amino acid sequences (5) to (7) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (5) to (7):
(5)
MKRQKRFEELEKRPIHQDTFVKEWPEEGFV
AMMGPNDPKPSVKVENGKIVEMDGKKLEDF
DLIDLYIAKYGINIDNVEKVMNMDSTKIAR
MLVDPNVSRDEIIEITSALTPAKAEEIISK
LDFGEMIMAVKKMRPRRKPDNQCHVTNTVD
NPVQIAADAADAALRGFPEQETTTAVARYA
PFNAISILIGAQTGRPGVLTQCSVEEATEL
QLGMRGFTAYAETISVYGTDRVFTDGDDTP
WSKGFLASCYASRGLKMRFTSGAGSEVLMG
YPEGKSMLYLEARCILLTKASGVQGLQNGA
VSCIEIPGAVPNGIREVLGENLLCMMCDIE
CASGCDQAYSHSDMRRTERFIGQFIAGTDY
INSGYSSTPNYDNTFAGSNTDAMDYDDMYV
MERDLGQYYGIHPVKEETIIKARNKAAKAL
QAVFEDLGLPKITDEEVEAATYANTHDDMP
KRDMVADMKAAQDMMDRGITAIDIIKALYN
HGFKDVAEAILNLQKQKVVGDYLQTSSIFD
KDWNVTSAVNDGNDYQGPGTGYRLYEDKEE
WDRIKDLPFALDPEHLEL
(6)
MADIDENLLRKIVKEVLSETNQIDTKIDFD
KSNDSTATATQEVQQPNSKAVPEKKLDWFQ
PVGEAKPGYSKDEVVIAVGPAFATVLDKTE
TGIPHKEVLRQVIAGIEEEGLKARVVKVYR
SSDVAFCAVQGDHLSGSGIAIGIQSKGTTV
IHQKDQDPLGNLELFPQAPVLTPETYRAIG
KNAAMYAKGESPEPVPAKNDQLARIHYQAI
SAIMHIRETHQVVVGKPEEEIKVTFD
(7)
MSEVDDLVAKIMAQMGNSSSANSSTGTSTA
STSKEMTADDYPLYQKHRDLVKTPKGHNLD
DINLQKVVNNQVDPKELRITPEALKLQGEI
AANAGRPAIQKNLQRAAELTRVPDERVLEM
YDALRPFRSTKQELLNIAKELRDKYDANVC
AAWFEEAADYYESRKKLKGDN
4 . The anti-obesity agent according to any one of claims 1 to 3 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces an enteroadherent protein depicted in the following amino acid sequence (8) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (8):
(8)
MFGHDGRIVTKVYQWAGTYYYFDPNTYLRV
DNDYRQSQWGDWYMFGPDGRIVTGLKEWYG
SYYYFDPTTYLKVTNKWIDNKYFGPAGQQA
ISRFERLDNKYYYFDANGAVLNIHDQFKNI
DNHTYYFGADGACYTSQFLNKDGKQYYFDN
DGIMLTDQEKIIDGKFYHFNVNGEAIQVND
PSEI
5 . The anti-obesity agent according to any one of claims 1 to 4 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme depicted in any of the following amino acid sequences (16) to (20) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (16) to (20):
(16)
MKAAVINDPVDGFVTVKDVQLRDLKPGEAL
VDMEYCGLCHTDLHVAAGDFGKKPGRIIGH
EGVGRVSKVAPGVTSLKVGDRVSIAWFFKG
CGHCEYCLTGRETLCRNVLNAGYTADGAMA
EQCIVPADYAVKVPEGLDPVEATSLTCAGV
TMYKALKVADIKPGQWVSIVGAGGLGNLGI
QLAHNVFGAHVIAVDGNPDKLEAAKKNGAE
ILINRHDGDVDKQIQEKVGGVHAAVVTAVS
ASAFDQAVDSLRPDGKLVAVALPQGDMKLN
IAKTVLDGIIVAGSLVGTRQDLAECFQFGA
EGKVHPIVKTRKLSEINDMIQELKDNKVVG
RNVVDFVHNDND
(17)
MEKRENAIPKTMKAWAVTTPGPIDGKESPI
EFTEKPVPTPKRGEVLVKVITCGVCHTDLH
VTEGDLPVHHEHVTPGHEIVGKVVGFGPET
QRFKFGERIGIPWFRHACGVCKFCRSGHEN
LCPHSLYTGWDHDGGYAEYVTVPEGFAYRL
PEKFDSLEAAPLLCAGIIGYRAFERANVPA
GGRLGLYGFGGSAHITAQIALAQGIEVHVF
TRGEDAKKFALELGCASVQGSYDPAPVPLD
SSIIFAPVGDMVLPALASLVPGGTLALAGI
HMTDIPTMNYQKEIFHEKTLTSVESNTRRD
GEEFLTLADRLNIHPEVHEYPLAKADEALR
YVKHGDIKGACVLRVSED
(18)
MQIKAALATKPNADLEIQTVELDEPKENEV
LIKIASTGFCHTDIVGRSGATTPLPVVLGH
EGAGVVQKVGANVTDVKPGDHVVLSFSYCG
HCYNCTHNHQGLCENFNQLNFEGKTYDGTH
RLHLDDGTPVSVFFGQSSFATYVTANVHNI
VKVDQDVDLNLLGPLGCGMQTGAGTVLNYI
KPAPEDAIAVFGAGAVGLAAIMAAKIAGVK
HIIAINRNGNHLDLAKELGATETINNTAED
PVKAIKEIVPRGVTYAIDTTGNTGVIKSAI
DSLATAGECVLLGVGGDITLDLMNDILSES
KKISGVVEGDSNPQEFIPQLVKYYKQSKFP
LDKLVKYYDFADINQVIADSTNGKVIKPII
KIDPELAKLPLTNDGSNVQKMVAEAGLADQ
ITIDSAGTSNIAEGSPADSRTKAILDKYHI
KDDGMIARQLQDRDYYDADYIIAMDQMNVR
DAKDMAPAGLENKVHGIFEATPGKENCYIV
DPWITH
(19)
MKKAIFEKAGQMKIVDVDRPTIEKPDDVII
KVVRTCVCGSDLWNFRGINPVEKDSENSGH
EAIGIVEEVGEDITTVKPGDFVIAPFTHGC
GHCAACRAGFDGSCQSHNDNFSSGVQAQYV
RFQHGQWALVKVPGKPSDYSEGMLKSLLTL
ADVMATGYHAARVANVSDGDTVVVMGDGAV
GLCAIIAAKMRGAKKIISTSRHADRQALAK
EFGATDNVAERSDEAVQKIMELTNGAGADA
VLECVGTEQSTDTAMKVGRPGTIVGRVGLP
HTPKMDMTVLFYNNTIVGGGPASVTTYDKD
VLLKAVLDGDINPGKVFTKSFDLDQIQEAY
EAMDKREAIKSYIIMDGFERD
(20)
MGRLDNKVAIITGGSKGIGAAVAKKFIEEG
AKVVLTARKMDEGQKVADQLGDNAIFIQQD
VARKGDWDRVIRQTVQVFGKLNIVVNNAGI
AEYADVEKTDAEIWDKTIAVNLTGTMWGTK
LGIEAMKNNGEKNSIINMSSIEGLIGDPDL
FAYNASKGGVRLLTKSAALDCARKGYDIRV
NTIHPGYISTPLVDNLVKDDPKAEGHLESL
HPLGRLGKPEEIANLALYLASDESSFSTGS
EFVADGGYTAQ
6 . The anti-obesity agent according to any one of claims 1 to 5 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme depicted in the following amino acid sequence (21) or (22) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (21) or (22):
(21)
MTNVPTVKLNNGVEMPTLGFEVFQVPDLSQ
AEQAVTDALEVGYRLIDTAAAYQNEEAVGK
AIKNSSVNREDVFVTSKLWVSDFNYKRAKA
GIDASLQKLGLDYMDLYLLHQPYGDTMGAW
RALQEAQKEGKIRAIGVSNFYADQLKDLEL
TMPVKPAVNQIEVNPWYQQDQEVKFAQSED
IRVEAWAPFAEGKHDIFTNEIIAEIAAKYG
KSNGQVILRWLLQRGITVIPKSVHKNRMEE
NIDVFDFELSNDDMKKIASLNKKESQFFDH
RDPVTIEQIFGSSLKMVQDDEK
(22)
MILDETITLNSGVKIPKFALGTWMIDDDQA
AEAVRNAIKMGYRHIDTAQAYDNERGVGEG
VRTAGIDRDKIFVTSKIAAEHKDYDVTKKS
IDETLEKMGLDYIDMMLIHSPQPWKEVNQS
DNRYLEGNLAAWRAMEDAVNEGKIRTIGVS
NFKKADLENIIKNSDTVPAVDQVLAHIGHT
PFNLLSFTHEHDIAVEAYSPVAHGAALDNP
VIEKMAKKYNVSVPQLCIRYDWQIGMIVLP
KTTNPEHMKENTEIDFEISEADMDLLRRVK
PLDYGDFDIYPVYGGKM
7 . The anti-obesity agent according to any one of claims 1 to 6 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces an aldehyde dehydrogenase depicted in any of the following amino acid sequences (23) to (25) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (23) to (25):
(23)
MPANNKKQVEKKELTAEEKKQNAQKLVDDL
MTKSQAAFEKLRYYSQEQVDKICQAMALAA
EEHHMDLAVDAANETGRGVAEDKAIKNIYA
SEYIWNNIRHDKTVGIIEDNDEDQTIKIAD
PLGVIAGIVPVTNPTSTTIFKSIISAKTRN
TIIFSFHRQAMKSSIKTAKILQEAAEKAGA
PKNMIQWLPESTRENTTALLQHPNTATILA
TGGPSLVKAAYSSGNPALGVGPGNGPAYIE
KTANIERSVYDIVLSKTFDNGMICATENSV
VVDEEIYDKVKEEFQKWNCYFLKPNEIDKF
TDGFIDPDRHQVRGPIAGRSANAIADMCGI
KVPDNTKVIIAEYEGVGDKYPLSAEKLSPV
LTMYKATSHENAFDICAQLLHYGGEGHTAA
IHTLDDDLATKYGLEMRASRIIVNSPSGIG
GIGNIYNNMTPSLTLGTGSYGSNSISHNVT
DWDLLNIKTIAKRRENRQWVKIPPKVYFQR
NSLKELQDIPNINRAFIVTGPGMSKRGYVQ
RVIDQLRQRQNNTAFLVFDDVEEDPSTNTV
EKGVAMMNDFKPDTIIALGGGSPMDAAKAM
WMFYEHPETSWYGVMQKYLDIRKRAYQIKK
PTKSQLIGIPTTSGTGSEVTPFAVITDSKT
HVKYPLADYALTPNIAIVDSQFVETVPAKT
TAWTGLDVLCHATESYVSVMATDYTRGWSL
QTIKGVMENLPKSVQGDKLARRKMHDFSTM
AGMAFGQAFLGINHSLAHKMGGAFGLPHGL
LIAIAMPQVIRFNAKRPQKLALWPHYETYH
ATKDYADIARFIGLKGNTDEELAEAYAKKV
IELAHECGVKLSLKDNGVTREEFDKAVDDL
ARLAYEDQCTTTNPVEPLVSQLKELLERCY
DGTGVEEK
(24)
MAYQSINPFTNQVEKTFENTTDEELEQTLT
TAHQLYLDWRKYNDLEERKRQILKLGQILR
ERRVEYATVMSKEMGKLISEAEGEVDLCAS
FCDYYAAHADEFLQPKIIATTSGRAKVLKQ
SLGILVAVEPWNFPFYQIARVFIPNFIAGN
PMILKDASNCPASAQAFNDAVKEAGAPAGS
LTNLFLSYDQVNKAIADKRVAGVCLTGSER
GGATVAKEAGANLKKSTLELGGNDAFIILD
DADWDLVEKVAPAARLYNAGQVCTSSKRFI
VLEKDYDRFLKMMKDAFSKVKMGDPLDPLT
TLAPLSSKKAKEKLQQQVATAVENGAKVYY
GNKPVDMEGQFFMPTILTDITPDNPIFDTE
MFGPVASVYKVSSEEEAIELANNSSYGLGN
TIFSNDSEHAERVAAKIETGMSWINAGWAS
LPELPFGGVKNSGYGRELSSYGIDEFTNKH
LIYEARQ
(25)
MQINDIESAVRKILAEELDNASSSSANVAA
TTDNGHRGIFTNVNDAIAAAKAAQEIYRDK
PIAVRQQVIDAIKEGFRPYIEKMAKDIKEE
TGMGTVEAKIAKLNNALYNTPGPEILEPVV
ENGDGGMVMYERLPYGVIGAVGPSTNPSET
VIANAIMMLAGGNTLYFGAHPGAKNVTRWT
IEKMNDFIADATGLHNLVVSIETPTIESVQ
QMMKHPDIAMLAVTGGPAVVHQAMTSGKKA
VGAGPGNPPAMVDATADIDLAAHNIITSAS
FDNDILCTAEKEVVAESSIKDELIRKMQDE
GAFVVNREQADKLADMCIQENGAPDRKFVG
KDATYILDQANIPYTGHPVEIICELPKEHP
LVMTEMLMPILPVVSCPTFDDVLKTAVEVE
KGNHHTATIHSNNLKHINNAAHRMQCSIFV
VNGPSYVGTGVADNGAHSGASALTIATPTG
EGTCTARTFTRRVRLNSPQGFSVRNWY
8 . The anti-obesity agent according to any one of claims 1 to 7 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerate kinase depicted in the following amino acid sequence (26) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (26):
(26)
MKFVIAPDSFKGGLTAKEAANVMAEGIKRV
FPNAEYALVPMADGGEGTVQSLVDATNGQK
MIAKVHNPLNKLVNAEYGILGDGETAVIEM
AAASGLQFVNKETANPLITTTYGTGELIKD
ALDHNIKKIIIGIGGSATVDGGAGMAQALG
ARLLDADNHEIGLGGGELASLEQVDFGGLD
PRLKNVDIQIASDVTNPLTGKNGAAPVFGP
QKGADEEMVNILDKNLHHYARKIVAAGGPD
VEQTAGAGAAGGLGAGLIAFTGATMKRGVE
LVIEATQLQKKAVGADYVFTGEGGIDFQTK
FGKTPYGVAKATKEVAPTAPVIVLAGNIGK
GVNDLYSSTAIDAIFATPEGAKPLKTALAD
APIDIAQTAENVARLIKVSHVSN
9 . The anti-obesity agent according to any one of claims 1 to 8 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol kinase depicted in the following amino acid sequence (27) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (27):
(27)
LSEQQYIMAIDQGTTSSRAIIFDHDGNKVA
ISQQEFPQYFPQPGWVEHDPLEIWDSVQSV
ISNVMIKSQIKPYKIAAIGITNQRETTVIW
DRHTGKPIYNAIVWQSKQTSDIAEQLIKDG
YKDMIHQKTGLVIDSYFAATKIKWILDHVP
GAREKAAKGDLMFGTIDTWLLWNLSGRRVH
ATDVTNASRTMLFNIHTLDWDQDILDLLDI
PQSLLPVVKPSSAIYGYTGDYHFYGVQIPI
AGIAGDQQAALFGQAAYDKGSIKNTYGTGA
FIVMNTGLKPTLSDNGLLTTIAYGLDGQTH
YALEGSIFVAGSAVQWLRDGLKMFDKASES
EQMAVDAKTTGGVYVVPAFTGLGAPYWDQE
VRGAMFGLTRGTERGHIIRATLEAIAYQTK
DVVDTMVKDTQLPLTALTVNGGASRNNFMM
QFQADILQTPIKRAAMEETTALGAAFLAGL
AVDFWEDQDELRKLSRIGDQFDPQMDPQKA
ADLYRGWQRAIAAAQFYGKD
10 . The anti-obesity agent according to any one of claims 1 to 9 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-3-phosphate dehydrogenase depicted in the following amino acid sequence (28) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (28):
(28)
MAEKIAVLGAGSWGSVLANMLTENGHDVTL
WSRNEEQVKQLNTEHTNPRYMKDFVYSTNL
TATTDMKKAVKGASVVLIVIPTKGLREVAK
QLNAILTELHQKPLVIHATKGLEQNTYKRP
SEMLSEDISPENRQAIVVLSGPSHAEDVAI
KDMTAVTAACEDLASAKKAQKLFSNSYFRV
YTNDDVIGAEFGAALKNIIAIGAGAIQGLG
YHDNARAALITRGLAEIRRLGVAFGANPMT
FIGLSGVGDLVVTATSKNSRNWRAGYQLGQ
GKKLQDVIDNMGMVIEGVYTTKAAYELSRK
RQVQMPITEALYRVLYEGEDIKTAISQLMD
RDLTSENE
11 . The anti-obesity agent according to any one of claims 1 to 10 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a triosephosphate isomerase depicted in any of the following amino acid sequences (29) to (31) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (29) to (31):
(29)
MRKPFIAANWKMHKNVQESVEFVDAIKGKL
PDPQEVEVGIAAQAFALPSMVQAADDSGLK
IIAQNAAAEYSGAFTGEISLRGLADAGVSY
VMLGHIERRHLFHEDNELVNRKVLAALQMG
VTPIICTDETMVQKEVNGEIHYVFQQLMSV
LRGVSLDQIKNVVVSYEPSWAVGYGQHANP
VLAEEGCRQIRRTIADNYTYEIADKIRILY
GGSVNPDNIGMIMNKPDVDGVLIGRASLDV
DNFLRMVNYLKNDQEK
(30)
MRKPFIIANWKMNKNVHESVAFVKAIKEKL
PADKEIGIAAQAVSLYNMKKVASSSNLQII
AQNASAELEGPYTGEISMRSLADAGVTYVM
LGHLERRRLFNESNDSINQKVLAALNAGII
PIICTDEEMVQTEVNGQIHYVFRQLKSVLK
GVPANKLSQIVISYEPSWAVGSTHQANPDI
AEEGCQAIRQSLVEMYGNEIGEQVRILYGG
SVNPENIGQIMSKPNVDGALIGRASLEIES
FLQMINYIELASKQKLQVI
(31)
MRVPIIAGNWKMHKDVQEAVSFIEKVKNQL
PPADQLETAIAAPTLCLVPMVKAAEESPLK
IMAENCYYKNEGAYTGETSPYALYQAGIHH
VILGHSERRTYFNETDELINKKVKAALVNG
LCPIVCCDDTMRRRVAGKKVHWVVSRILAD
LHGLTNDEICHVTVAYEPSWAIGTGESADP
EQAAEGCYLIRQTISDMYGDEVANNVRILY
GGSVTTSNINALMAKNDIDGVLVGAASLNP
ETFLQLVHH
12 . The anti-obesity agent according to any one of claims 1 to 11 , wherein the microorganism belonging to the species Lactobacillus reuteri is Lactobacillus reuteri JCM1112T.
13 . An anti-obesity food and drink comprising, as an active ingredient, a microorganism belonging to the species Lactobacillus reuteri and capable of producing lipases depicted in the following amino acid sequences (1) to (3) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (1) to (3):
(1)
MVKLMTIHELANNPTLSGQVRLIENIVYGA
MDGEALHMSILAPWTQRFPKQYQTEPRPLI
VFVQGSSWRTPKMGEEIPQLVQFVRAGYIV
ATVQHRSSIDSHPFPAFLQDVKTAIRFLRA
NAQKYAIDPQQVAIWGTSSGANAAMLVGLT
GDDPRYKVDLYQDESDAVDAVVSCFAPMDV
EKTFEYDANVPGNKLLQYCLLGPDVSKWPE
IEKQMSPLYQVKDGQNYPPFLLFHGDADKV
VPYEQMEKMYMRLKDNGNSVEAYRVKGANH
ERDFWSPTIYNIVQKFLGDQFK
(2)
LIYVLKDLCNTIAEVYGKSILKGVFIMKHT
LKVDQVRDGLWLDSDITYTQVPGWLGNTTR
DLKLSVIRHFQTNDDTRYPVIFWFAGGGWM
DTDHNVHLPNLVDFARHGYIVVGVEYRDSN
KVQFPGQLEDAKAAIRYMRANAKRFQADPN
RFIVMGESAGGHMASMLGVTNGLNQFDKGA
NLDYSSDVQVAVPFYGVVDPLTAKTGSASN
DFDFVYRNLLGAEPENAPELDSAANPLTYV
NSNSTPFLIFHGTEDVVVPIKDSEKLYDAL
VENNVPAELYEIEGASHMDVKFLQPQVFKI
VMDFLDKYLTRS
(3)
MEIKSVNLDQPYSSLDIYHSNTDKALPGLV
ILPGGSYNQIMERDSERVALTFATHAWQTF
VVRYPVVEHKNYEEAKIAVHQAFEYIVNHA
AELDVDADRLGIIGFSAGGQIAAAYSNEKL
THARFAALGYPVIQPLIDERMGVTTENVAK
LVNPQTPPTFMWGSAKDELTPFVDHLQVYA
DALIKNDIPYELHEFGTGGHGIALANEYTG
IVNNDRVDNHMGKWFPLFLEWLTELNLI
14 . The anti-obesity food and drink according to claim 13 , wherein the microorganism belonging to the species Lactobacillus reuteri has nucleotide sequences encoding the amino acid sequences (1) to (3) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (1) to (3), and a nucleotide sequence depicted in the following formula (4):
(4)
ATGCATGGATTTATTGGCGAATTTTTTGGCACCATGGTTTTAATCCTATTAGGAGCAGGA
TGTTGTGCTGGTAATAGTTTGAATAAAACATATGGGAAACAAAGTGGCTGGTGGTTTATC
TGTATTTCATGGGGCTTAGCAGTTACAATGGGAGTTTATGTTGCAGGATTTCTGGGTTCA
TTAGGGCACTTAAATCCCGCTGTAACAATTCCTTTTGCTATTTTTGGCTTATTCCCATGG
AGTAACGTTATACCTTACTTACTTGGTCAATTTCTTGGTGCGTTTGTTGGTGCAGTATTA
GTAATTATTCAATTCTATCCACAATTTAAAGCAACCCCAAATGAAGAAGAAGGAAATAAT
GTTGGTATTTTTGCTACTCGTCCAGCGATAAATAGTCCAATTTTTAACTTTTTCTCAGAA
GTGATTGCGACCTTTGCATTTATTTTCATCTTATTAAATCTTGGCAACTTTACACAGGGA
TTGAAGCCATTTATCGTAGGAATGGTTATTGCAGTTGTTGGTACATGTCTCGGGACAACT
ACTGGCTTTGCATTAAACCCAGCTCGTGATTGGTCACCACGTTTAGCATATACTATTTTG
CCAATTCCTAATAAGGGTGTTTCAGAATGGTGGTATGCATGGGTTCCAATGTGTGGCCCA
ATTGTTGGGGGCCTTCTTGCTTGTGCTTTACAAACGGCACTAGTTTAG
15 . The anti-obesity food and drink according to claim 13 or 14 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme composed of subunits depicted in the following amino acid sequences (5) to (7) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (5) to (7):
(5)
MKRQKRFEELEKRPIHQDTFVKEWPEEGFV
AMMGPNDPKPSVKVENGKIVEMDGKKLEDF
DLIDLYIAKYGINIDNVEKVMNMDSTKIAR
MLVDPNVSRDEIIEITSALTPAKAEEIISK
LDFGEMIMAVKKMRPRRKPDNQCHVTNTVD
NPVQIAADAADAALRGFPEQETTTAVARYA
PFNAISILIGAQTGRPGVLTQCSVEEATEL
QLGMRGFTAYAETISVYGTDRVFTDGDDTP
WSKGFLASCYASRGLKMRFTSGAGSEVLMG
YPEGKSMLYLEARCILLTKASGVQGLQNGA
VSCIEIPGAVPNGIREVLGENLLCMMCDIE
CASGCDQAYSHSDMRRTERFIGQFIAGTDY
INSGYSSTPNYDNTFAGSNTDAMDYDDMYV
MERDLGQYYGIHPVKEETIIKARNKAAKAL
QAVFEDLGLPKITDEEVEAATYANTHDDMP
KRDMVADMKAAQDMMDRGITAIDIIKALYN
HGFKDVAEAILNLQKQKVVGDYLQTSSIFD
KDWNVTSAVNDGNDYQGPGTGYRLYEDKEE
WDRIKDLPFALDPEHLEL
(6)
MADIDENLLRKIVKEVLSETNQIDTKIDFD
KSNDSTATATQEVQQPNSKAVPEKKLDWFQ
PVGEAKPGYSKDEVVIAVGPAFATVLDKTE
TGIPHKEVLRQVIAGIEEEGLKARVVKVYR
SSDVAFCAVQGDHLSGSGIAIGIQSKGTTV
IHQKDQDPLGNLELFPQAPVLTPETYRAIG
KNAAMYAKGESPEPVPAKNDQLARIHYQAI
SAIMHIRETHQVVVGKPEEEIKVTFD
(7)
MSEVDDLVAKIMAQMGNSSSANSSTGTSTA
STSKEMTADDYPLYQKHRDLVKTPKGHNLD
DINLQKVVNNQVDPKELRITPEALKLQGEI
AANAGRPAIQKNLQRAAELTRVPDERVLEM
YDALRPFRSTKQELLNIAKELRDKYDANVC
AAWFEEAADYYESRKKLKGDN
16 . The anti-obesity food and drink according to any one of claims 13 to 15 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces an enteroadherent protein depicted in the following amino acid sequence (8) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (8):
(8)
MFGHDGRIVTKVYQWAGTYYYFDPNTYLRV
DNDYRQSQWGDWYMFGPDGRIVTGLKEWYG
SYYYFDPTTYLKVTNKWIDNKYFGPAGQQA
ISRFERLDNKYYYFDANGAVLNIHDQFKNI
DNHTYYFGADGACYTSQFLNKDGKQYYFDN
DGIMLTDQEKIIDGKFYHFNVNGEAIQVND
PSEI
17 . The anti-obesity food and drink according to any one of claims 13 to 16 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme depicted in any of the following amino acid sequences (16) to (20) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (16) to (20):
(16)
MKAAVINDPVDGFVTVKDVQLRDLKPGEAL
VDMEYCGLCHTDLHVAAGDFGKKPGRIIGH
EGVGRVSKVAPGVTSLKVGDRVSIAWFFKG
CGHCEYCLTGRETLCRNVLNAGYTADGAMA
EQCIVPADYAVKVPEGLDPVEATSLTCAGV
TMYKALKVADIKPGQWVSIVGAGGLGNLGI
QLAHNVFGAHVIAVDGNPDKLEAAKKNGAE
ILINRHDGDVDKQIQEKVGGVHAAVVTAVS
ASAFDQAVDSLRPDGKLVAVALPQGDMKLN
IAKTVLDGIIVAGSLVGTRQDLAECFQFGA
EGKVHPIVKTRKLSEINDMIQELKDNKVVG
RNVVDFVHNDND
(17)
MEKRENAIPKTMKAWAVTTPGPIDGKESPI
EFTEKPVPTPKRGEVLVKVITCGVCHTDLH
VTEGDLPVHHEHVTPGHEIVGKVVGFGPET
QRFKFGERIGIPWFRHACGVCKFCRSGHEN
LCPHSLYTGWDHDGGYAEYVTVPEGFAYRL
PEKFDSLEAAPLLCAGIIGYRAFERANVPA
GGRLGLYGFGGSAHITAQIALAQGIEVHVF
TRGEDAKKFALELGCASVQGSYDPAPVPLD
SSIIFAPVGDMVLPALASLVPGGTLALAGI
HMTDIPTMNYQKEIFHEKTLTSVESNTRRD
GEEFLTLADRLNIHPEVHEYPLAKADEALR
YVKHGDIKGACVLRVSED
(18)
MQIKAALATKPNADLEIQTVELDEPKENEV
LIKIASTGFCHTDIVGRSGATTPLPVVLGH
EGAGVVQKVGANVTDVKPGDHVVLSFSYCG
HCYNCTHNHQGLCENFNQLNFEGKTYDGTH
RLHLDDGTPVSVFFGQSSFATYVTANVHNI
VKVDQDVDLNLLGPLGCGMQTGAGTVLNYI
KPAPEDAIAVFGAGAVGLAAIMAAKIAGVK
HIIAINRNGNHLDLAKELGATETINNTAED
PVKAIKEIVPRGVTYAIDTTGNTGVIKSAI
DSLATAGECVLLGVGGDITLDLMNDILSES
KKISGVVEGDSNPQEFIPQLVKYYKQSKFP
LDKLVKYYDFADINQVIADSTNGKVIKPII
KIDPELAKLPLTNDGSNVQKMVAEAGLADQ
ITIDSAGTSNIAEGSPADSRTKAILDKYHI
KDDGMIARQLQDRDYYDADYIIAMDQMNVR
DAKDMAPAGLENKVHGIFEATPGKENCYIV
DPWITH
(19)
MKKAIFEKAGQMKIVDVDRPTIEKPDDVII
KVVRTCVCGSDLWNFRGINPVEKDSENSGH
EAIGIVEEVGEDITTVKPGDFVIAPFTHGC
GHCAACRAGFDGSCQSHNDNFSSGVQAQYV
RFQHGQWALVKVPGKPSDYSEGMLKSLLTL
ADVMATGYHAARVANVSDGDTVVVMGDGAV
GLCAIIAAKMRGAKKIISTSRHADRQALAK
EFGATDNVAERSDEAVQKIMELTNGAGADA
VLECVGTEQSTDTAMKVGRPGTIVGRVGLP
HTPKMDMTVLFYNNTIVGGGPASVTTYDKD
VLLKAVLDGDINPGKVFTKSFDLDQIQEAY
EAMDKREAIKSYIIMDGFERD
(20)
MGRLDNKVAIITGGSKGIGAAVAKKFIEEG
AKVVLTARKMDEGQKVADQLGDNAIFIQQD
VARKGDWDRVIRQTVQVFGKLNIVVNNAGI
AEYADVEKTDAEIWDKTIAVNLTGTMWGTK
LGIEAMKNNGEKNSIINMSSIEGLIGDPDL
FAYNASKGGVRLLTKSAALDCARKGYDIRV
NTIHPGYISTPLVDNLVKDDPKAEGHLESL
HPLGRLGKPEEIANLALYLASDESSFSTGS
EFVADGGYTAQ
18 . The anti-obesity food and drink according to any one of claims 13 to 17 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-degrading enzyme depicted in the following amino acid sequence (21) or (22) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (21) or (22):
(21)
MTNVPTVKLNNGVEMPTLGFEVFQVPDLSQ
AEQAVTDALEVGYRLIDTAAAYQNEEAVGK
AIKNSSVNREDVFVTSKLWVSDFNYKRAKA
GIDASLQKLGLDYMDLYLLHQPYGDTMGAW
RALQEAQKEGKIRAIGVSNFYADQLKDLEL
TMPVKPAVNQIEVNPWYQQDQEVKFAQSED
IRVEAWAPFAEGKHDIFTNEIIAEIAAKYG
KSNGQVILRWLLQRGITVIPKSVHKNRMEE
NIDVFDFELSNDDMKKIASLNKKESQFFDH
RDPVTIEQIFGSSLKMVQDDEK
(22)
MILDETITLNSGVKIPKFALGTWMIDDDQA
AEAVRNAIKMGYRHIDTAQAYDNERGVGEG
VRTAGIDRDKIFVTSKIAAEHKDYDVTKKS
IDETLEKMGLDYIDMMLIHSPQPWKEVNQS
DNRYLEGNLAAWRAMEDAVNEGKIRTIGVS
NFKKADLENIIKNSDTVPAVDQVLAHIGHT
PFNLLSFTHEHDIAVEAYSPVAHGAALDNP
VIEKMAKKYNVSVPQLCIRYDWQIGMIVLP
KTTNPEHMKENTEIDFEISEADMDLLRRVK
PLDYGDFDIYPVYGGKM
19 . The anti-obesity food and drink according to any one of claims 13 to 18 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces an aldehyde dehydrogenase depicted in any of the following amino acid sequences (23) to (25) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (23) to (25):
(23)
MPANNKKQVEKKELTAEEKKQNAQKLVDDL
MTKSQAAFEKLRYYSQEQVDKICQAMALAA
EEHHMDLAVDAANETGRGVAEDKAIKNIYA
SEYIWNNIRHDKTVGIIEDNDEDQTIKIAD
PLGVIAGIVPVTNPTSTTIFKSIISAKTRN
TIIFSFHRQAMKSSIKTAKILQEAAEKAGA
PKNMIQWLPESTRENTTALLQHPNTATILA
TGGPSLVKAAYSSGNPALGVGPGNGPAYIE
KTANIERSVYDIVLSKTFDNGMICATENSV
VVDEEIYDKVKEEFQKWNCYFLKPNEIDKF
TDGFIDPDRHQVRGPIAGRSANAIADMCGI
KVPDNTKVIIAEYEGVGDKYPLSAEKLSPV
LTMYKATSHENAFDICAQLLHYGGEGHTAA
IHTLDDDLATKYGLEMRASRIIVNSPSGIG
GIGNIYNNMTPSLTLGTGSYGSNSISHNVT
DWDLLNIKTIAKRRENRQWVKIPPKVYFQR
NSLKELQDIPNINRAFIVTGPGMSKRGYVQ
RVIDQLRQRQNNTAFLVFDDVEEDPSTNTV
EKGVAMMNDFKPDTIIALGGGSPMDAAKAM
WMFYEHPETSWYGVMQKYLDIRKRAYQIKK
PTKSQLIGIPTTSGTGSEVTPFAVITDSKT
HVKYPLADYALTPNIAIVDSQFVETVPAKT
TAWTGLDVLCHATESYVSVMATDYTRGWSL
QTIKGVMENLPKSVQGDKLARRKMHDFSTM
AGMAFGQAFLGINHSLAHKMGGAFGLPHGL
LIAIAMPQVIRFNAKRPQKLALWPHYETYH
ATKDYADIARFIGLKGNTDEELAEAYAKKV
IELAHECGVKLSLKDNGVTREEFDKAVDDL
ARLAYEDQCTTTNPVEPLVSQLKELLERCY
DGTGVEEK
(24)
MAYQSINPFTNQVEKTFENTTDEELEQTLT
TAHQLYLDWRKYNDLEERKRQILKLGQILR
ERRVEYATVMSKEMGKLISEAEGEVDLCAS
FCDYYAAHADEFLQPKIIATTSGRAKVLKQ
SLGILVAVEPWNFPFYQIARVFIPNFIAGN
PMILKDASNCPASAQAFNDAVKEAGAPAGS
LTNLFLSYDQVNKAIADKRVAGVCLTGSER
GGATVAKEAGANLKKSTLELGGNDAFIILD
DADWDLVEKVAPAARLYNAGQVCTSSKRFI
VLEKDYDRFLKMMKDAFSKVKMGDPLDPLT
TLAPLSSKKAKEKLQQQVATAVENGAKVYY
GNKPVDMEGQFFMPTILTDITPDNPIFDTE
MFGPVASVYKVSSEEEAIELANNSSYGLGN
TIFSNDSEHAERVAAKIETGMSWINAGWAS
LPELPFGGVKNSGYGRELSSYGIDEFTNKH
LIYEARQ
(25)
MQINDIESAVRKILAEELDNASSSSANVAA
TTDNGHRGIFTNVNDAIAAAKAAQEIYRDK
PIAVRQQVIDAIKEGFRPYIEKMAKDIKEE
TGMGTVEAKIAKLNNALYNTPGPEILEPVV
ENGDGGMVMYERLPYGVIGAVGPSTNPSET
VIANAIMMLAGGNTLYFGAHPGAKNVTRWT
IEKMNDFIADATGLHNLVVSIETPTIESVQ
QMMKHPDIAMLAVTGGPAVVHQAMTSGKKA
VGAGPGNPPAMVDATADIDLAAHNIITSAS
FDNDILCTAEKEVVAESSIKDELIRKMQDE
GAFVVNREQADKLADMCIQENGAPDRKFVG
KDATYILDQANIPYTGHPVEIICELPKEHP
LVMTEMLMPILPVVSCPTFDDVLKTAVEVE
KGNHHTATIHSNNLKHINNAAHRMQCSIFV
VNGPSYVGTGVADNGAHSGASALTIATPTG
EGTCTARTFTRRVRLNSPQGFSVRNWY
20 . The anti-obesity food and drink according to any one of claims 13 to 19 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerate kinase depicted in the following amino acid sequence (26) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (26):
(26)
MKFVIAPDSFKGGLTAKEAANVMAEGIKRV
FPNAEYALVPMADGGEGTVQSLVDATNGQK
MIAKVHNPLNKLVNAEYGILGDGETAVIEM
AAASGLQFVNKETANPLITTTYGTGELIKD
ALDHNIKKIIIGIGGSATVDGGAGMAQALG
ARLLDADNHEIGLGGGELASLEQVDFGGLD
PRLKNVDIQIASDVTNPLTGKNGAAPVFGP
QKGADEEMVNILDKNLHHYARKIVAAGGPD
VEQTAGAGAAGGLGAGLIAFTGATMKRGVE
LVIEATQLQKKAVGADYVFTGEGGIDFQTK
FGKTPYGVAKATKEVAPTAPVIVLAGNIGK
GVNDLYSSTAIDAIFATPEGAKPLKTALAD
APIDIAQTAENVARLIKVSHVSN
21 . The anti-obesity food and drink according to any one of claims 13 to 20 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol kinase depicted in the following amino acid sequence (27) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (27):
(27)
LSEQQYIMAIDQGTTSSRAIIFDHDGNKVA
ISQQEFPQYFPQPGWVEHDPLEIWDSVQSV
ISNVMIKSQIKPYKIAAIGITNQRETTVIW
DRHTGKPIYNAIVWQSKQTSDIAEQLIKDG
YKDMIHQKTGLVIDSYFAATKIKWILDHVP
GAREKAAKGDLMFGTIDTWLLWNLSGRRVH
ATDVTNASRTMLFNIHTLDWDQDILDLLDI
PQSLLPVVKPSSAIYGYTGDYHFYGVQIPI
AGIAGDQQAALFGQAAYDKGSIKNTYGTGA
FIVMNTGLKPTLSDNGLLTTIAYGLDGQTH
YALEGSIFVAGSAVQWLRDGLKMFDKASES
EQMAVDAKTTGGVYVVPAFTGLGAPYWDQE
VRGAMFGLTRGTERGHIIRATLEAIAYQTK
DVVDTMVKDTQLPLTALTVNGGASRNNFMM
QFQADILQTPIKRAAMEETTALGAAFLAGL
AVDFWEDQDELRKLSRIGDQFDPQMDPQKA
ADLYRGWQRAIAAAQFYGKD
22 . The anti-obesity food and drink according to any one of claims 13 to 21 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a glycerol-3-phosphate dehydrogenase depicted in the following amino acid sequence (28) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (28):
(28)
MAEKIAVLGAGSWGSVLANMLTENGHDVTL
WSRNEEQVKQLNTEHTNPRYMKDFVYSTNL
TATTDMKKAVKGASVVLIVIPTKGLREVAK
QLNAILTELHQKPLVIHATKGLEQNTYKRP
SEMLSEDISPENRQAIVVLSGPSHAEDVAI
KDMTAVTAACEDLASAKKAQKLFSNSYFRV
YTNDDVIGAEFGAALKNIIAIGAGAIQGLG
YHDNARAALITRGLAEIRRLGVAFGANPMT
FIGLSGVGDLVVTATSKNSRNWRAGYQLGQ
GKKLQDVIDNMGMVIEGVYTTKAAYELSRK
RQVQMPITEALYRVLYEGEDIKTAISQLMD
RDLTSENE
23 . The anti-obesity food and drink according to any one of claims 13 to 22 , wherein the microorganism belonging to the species Lactobacillus reuteri further produces a triosephosphate isomerase depicted in any of the following amino acid sequences (29) to (31) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in any of the amino acid sequences (29) to (31):
(29)
MRKPFIAANWKMHKNVQESVEFVDAIKGKL
PDPQEVEVGIAAQAFALPSMVQAADDSGLK
IIAQNAAAEYSGAFTGEISLRGLADAGVSY
VMLGHIERRHLFHEDNELVNRKVLAALQMG
VTPIICTDETMVQKEVNGEIHYVFQQLMSV
LRGVSLDQIKNVVVSYEPSWAVGYGQHANP
VLAEEGCRQIRRTIADNYTYEIADKIRILY
GGSVNPDNIGMIMNKPDVDGVLIGRASLDV
DNFLRMVNYLKNDQEK
(30)
MRKPFIIANWKMNKNVHESVAFVKAIKEKL
PADKEIGIAAQAVSLYNMKKVASSSNLQII
AQNASAELEGPYTGEISMRSLADAGVTYVM
LGHLERRRLFNESNDSINQKVLAALNAGII
PIICTDEEMVQTEVNGQIHYVFRQLKSVLK
GVPANKLSQIVISYEPSWAVGSTHQANPDI
AEEGCQAIRQSLVEMYGNEIGEQVRILYGG
SVNPENIGQIMSKPNVDGALIGRASLEIES
FLQMINYIELASKQKLQVI
(31)
MRVPIIAGNWKMHKDVQEAVSFIEKVKNQL
PPADQLETAIAAPTLCLVPMVKAAEESPLK
IMAENCYYKNEGAYTGETSPYALYQAGIHH
VILGHSERRTYFNETDELINKKVKAALVNG
LCPIVCCDDTMRRRVAGKKVHWVVSRILAD
LHGLTNDEICHVTVAYEPSWAIGTGESADP
EQAAEGCYLIPQTISDMYGDEVANNVRILY
GGSVTTSNINALMAKNDIDGVLVGAASLNP
ETFLQLVHH
24 . The anti-obesity food and drink according to any one of claims 13 to 23 , wherein the microorganism belonging to the species Lactobacillus reuteri is Lactobacillus reuteri JCM 1112T.
25 . A glycerol-degrading enzyme composed of subunits depicted in the following amino acid sequences (5) to (7) or amino acid sequences having deletion, substitution or addition of one or more amino acids in the amino acid sequences (5) to (7):
(5)
MKRQKRFEELEKRPIHQDTFVKEWPEEGFV
AMMGPNDPKPSVKVENGKIVEMDGKKLEDF
DLIDLYIAKYGINIDNVEKVMNMDSTKIAR
MLVDPNVSRDEIIEITSALTPAKAEEISK
LDFGEMIMAVKKMRPRRKPDNQCHVTNTVD
NPVQIAADAADAALRGFPEQETTTAVARYA
PFNAISILIGAQTGRPGVLTQCSVEEATEL
QLGMRGFTAYAETISVYGTDRVFTDGDDTP
WSKGFLASCYASRGLKMRFTSGAGSEVLMG
YPEGKSMLYLEARCILLTKASGVQGLQNGA
VSCIEIPGAVPNGIREVLGENLLCMMCDIE
CASGCDQAYSHSDMRRTERFIGQFIAGTDY
INSGYSSTPNYDNTFAGSNTDAMDYDDMYV
MERDLGQYYGIHPVKEETIIKARNKAAKAL
QAVFEDLGLPKITDEEVEAATYANTHDDMP
KRDMVADMKAAQDMMDRGITAIDIIKALYN
HGFKDVAEAILNLQKQKVVGDYLQTSSIFD
KDWNVTSAVNDGNDYQGPGTGYRLYEDKEE
WDRIKDLPFALDPEHLEL
(6)
MADIDENLLRKIVKEVLSETNQIDTKIDFD
KSNDSTATATQEVQQPNSKAVPEKKLDWFQ
PVGEAKPGYSKDEVVIAVGPAFATVLDKTE
TGIPHKEVLRQVIAGIEEEGLKARVVKVYR
SSDVAFCAVQGDHLSGSGIAIGIQSKGTTV
IHQKDQDPLGNLELFPQAPVLTPETYRAIG
KNAAMYAKGESPEPVPAKNDQLARIHYQAI
SAIMHIRETHQVVVGKPEEEIKVTFD
(7)
MSEVDDLVAKIMAQMGNSSSANSSTGTSTA
STSKEMTADDYPLYQKHRDLVKTPKGHNLD
DINLQKVVNNQVDPKELRITPEALKLQGEI
AANAGRPAIQKNLQRAAELTRVPDERVLEM
YDALRPFRSTKQELLNIAKELRDKYDANVC
AAWFEEAADYYESRKKLKGDN
26 . Nucleotide sequences encoding the glycerol-degrading enzyme subunits according to claim 25 , which are depicted in the following (9) to (11):
(10)
ATGGCTGATATTGATGAAAACTTATTACGTAAAATCGTTAAAGAAGTTTTAAGCGAAACT
AATCAAATCGATACTAAGATTGACTTTGATAAAAGTAATGATAGTACTGCAACAGCAACT
CAAGAGGTGCAACAACCAAATAGTAAAGCTGTTCCAGAAAAGAAACTTGACTGGTTCCAA
CCAGTTGGAGAAGCAAAACCTGGATATTCTAAGGATGAAGTTGTAATTGCAGTCGGTCCT
GCATTCGCAACTGTTCTTGATAAGACAGAAACTGGTATTCCTCATAAAGAAGTGCTTCGT
CAAGTTATTGCTGGTATTGAAGAAGAAGGGCTTAAGGCGCGGGTAGTTAAAGTTTACCGG
AGTTCAGATGTAGCATTCTGTGCTGTCCAAGGTGATCACCTTTCTGGTTCAGGAATTGCT
ATTGGTATCCAATCAAAAGGGACGACAGTTATTCACCAAAAGGATCAAGACCCTCTTGGT
AACCTTGAGTTATTCCCACAAGCGCCAGTACTTACTCCCGAAACTTATCGTGCAATTGGT
AAGAATGCCGCTATGTATGCTAAGGGTGAATCTCCAGAACCAGTTCCAGCTAAAAACGAT
CAACTTGCTCGTATTCACTATCAAGCTATTTCAGCAATTATGCATATTCGTGAAACTCAC
CAAGTTGTTGTTGGTAAGCCTGAAGAAGAAATTAAGGTTACGTTTGATTAA
(11)
ATGAGTGAAGTTGATGATTTAGTAGCAAAGATCATGGCTCAGATGGGAAACAGTTCATCT
GCTAATAGCTCTACAGGTACTTCAACTGCAAGTACTAGTAAGGAAATGACAGCAGATGAT
TACCCACTTTATCAAAAGCACCGTGATTTAGTAAAAACACCAAAAGGACACAATCTTGAT
GACATCAATTTACAAAAAGTAGTAAATAATCAAGTTGATCCTAAGGAATTACGGATTACA
CCAGAAGCATTGAAACTTCAAGGTGAAATTGCAGCTAATGCTGGCCGTCCAGCTATTCAA
AAGAATCTTCAACGAGCTGCAGAATTAACACGAGTACCTGACGAACGGGTTCTTGAAATG
TATGATGCATTGCGTCCTTTCCGTTCAACTAAGCAAGAATTATTGAACATTGCAAAGGAA
TTACGGGACAAGTATGACGCTAATGTTTGCGCAGCATGGTTTGAAGAAGCTGCTGATTAT
TATGAAAGTCGTAAGAAGCTAAAGGGCGATAACTAA
27 . An enteroadherent protein depicted in the following amino acid sequence (8) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (8):
(8)
MFGHDGRIVTKVYQWAGTYYYFDPNTYLRV
DNDYRQSQWGDWYMFGPDGRIVTGLKEWYG
SYYYFDPTTYLKVTNKWIDNKYFGPAGQQA
ISRFERLDNKYYYFDANGAVLNIHDQFKNI
DNHTYYFGADGACYTSQFLNKDGKQYYFDN
DGIMLTDQEKIIDGKFYHFNVNGEAIQVND
PSEI
28 . A nucleotide sequence encoding the enteroadherent protein according to claim 27 , which is depicted in the
(9)
ATGAAACGTCAAAAACGATTTGAAGAACTAGAAAAACGGCCAATTCATCAAGATACATTT
GTTAAAGAATGGCCAGAAGAAGGTTTCGTTGCAATGATGGGGCCTAATGACCCTAAGCCT
AGTGTAAAAGTTGAAAATGGCAAGATCGTAGAGATGGATGGTAAAAAGCTCGAAGATTTT
GATTTGATTGACTTGTACATTGCTAAGTATGGAATCAATATTGACAACGTTGAAAAAGTT
ATGAATATGGATTCTACCAAGATTGCACGGATGCTTGTTGATCCTAATGTTTCTCGTGAT
GAAATTATTGAAATTACATCAGCTTTGACTCCTGCTAAGGCTGAAGAGATCATCAGTAAG
CTTGATTTTGGTGAAATGATTATGGCTGTCAAGAAGATGCGCCCACGTCGTAAGCCTGAC
AACCAGTGTCACGTTACCAATACTGTTGATAACCCAGTTCAAATTGCTGCTGATGCTGCT
GATGCCGCTCTTCGTGGATTTCCAGAACAAGAAACCACGACAGCTGTGGCACGTTATGCA
CCATTCAATGCTATTTCAATTTTAATTGGTGCACAAACAGGTCGCCCTGGTGTATTGACA
CAATGTTCTGTTGAAGAAGCTACTGAATTGCAATTAGGTATGCGTGGTTTTACCGCATAT
GCTGAAACCATTTCAGTTTACGGTACTGATCGTGTATTTACCGATGGTGATGATACTCCA
TGGTCTAAAGGCTTCTTGGCATCTTGTTATGCATCACGTGGTTTGAAGATGCGATTTACT
TCAGGTGCCGGTTCAGAAGTTTTGATGGGTTATCCAGAAGGTAAGTCAATGCTTTACCTT
GAAGCGCGTTGTATTTTACTTACTAAGGCTTCAGGTGTTCAAGGACTTCAAAATGGTGCC
GTAAGTTGTATTGAAATTCCTGGTGCTGTTCCTAATGGTATTCGTGAAGTTCTCGGTGAA
AACTTGTTATGTATGATGTGTGACATCGAATGTGCTTCTGGTTGTGACCAAGCATACTCA
CACTCCGATATGCGGCGGACTGAACGGTTTATTGGTCAATTTATTGCCGGTACTGATTAT
ATTAACTCTGGTTACTCATCAACTCCTAACTACGATAATACCTTCGCTGGTTCAAACACT
GATGCTATGGACTACGATGATATGTATGTTATGGAACGTGACTTGGGTCAATATTATGGT
ATTCACCCTGTTAAGGAAGAAACCATTATTAAGGCACGTAATAAGGCCGCTAAAGCCCTT
CAAGCAGTATTTGAAGATCTTGGATTACCAAAGATTACTGATGAAGAGGTCGAAGCAGCA
ACGTATGCTAACACCCATGATGACATGCCAAAGCGGGATATGGTTGCAGATATGAAGGCT
GCTCAAGATATGATGGATCGTGGAATTACTGCTATTGATATTATCAAGGCATTGTACAAC
CACGGATTTAAAGATGTCGCTGAAGCAATTTTGAACCTTCAAAAACAAAAAGTTGTTGGT
GATTACCTTCAAACATCTTCTATTTTTGATAAAGATTGGAACGTCACTTCTGCTGTTAAC
GACGGAAATGATTATCAAGGACCAGGTACTGGATACCGTCTATATGAAGACAAGGAAGAA
TGGGATCGGATTAAAGACTTACCATTCGCCCTTGATCCAGAACATTTGGAACTGTAG
following (12):
(12)
ATGTTCGGTCACGATGGCCGCATTGTTACTAAAGTTTACCAATGGGCTGGCACGTATTAC
TACTTTGATCCGAATACTTATTTGCGAGTAGATAATGATTACCGTCAATCTCAGTGGGGC
GATTGGTATATGTTTGGCCCAGATGGTCGTATCGTTACAGGGTTAAAGGAATGGTACGGT
AGTTATTATTACTTTGATCCGACGACTTACTTAAAAGTAACTAATAAGTGGATAGATAAT
AAGTACTTTGGTCCAGCTGGTCAGCAAGCTATTTCACGCTTTGAGAGACTTGATAATAAG
TATTACTATTTCGATGCTAATGGGGCAGTTCTTAATATCCATGATCAATTTAAGAATATT
GATAACCACACTTATTACTTTGGAGCTGATGGTGCTTGTTATACCAGTCAATTCTTAAAT
AAGGATGGTAAACAGTATTATTTCGATAATGATGGAATTATGCTCACTGATCAAGAGAAG
ATCATTGACGGTAAATTCTATCATTTCAATGTTAATGGTGAAGCAATCCAAGTAAATGAT
CCTTCTGAAATTTGA
29 . A glycerol-degrading enzyme depicted in the following amino acid sequence (16) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (16):
(16)
MKAAVINDPVDGFVTVKDVQLRDLKPGEAL
VDMEYCGLCHTDLHVAAGDFGKKPGRIIGH
EGVGRVSKVAPGVTSLKVGDRVSIAWFFKG
CGHCEYCLTGRETLCRNVLNAGYTADGAMA
EQCIVPADYAVKVPEGLDPVEATSLTCAGV
TMYKALKVADIKPGQWVSIVGAGGLGNLGI
QLAHNVFGAHVIAVDGNPDKLEAAKKNGAE
ILINRHDGDVDKQIQEKVGGVHAAVVTAVS
ASAFDQAVDSLRPDGKLVAVALPQGDMKLN
IAKTVLDGIIVAGSLVGTRQDLAECFQFGA
EGKVHPIVKTRKLSEINDMIQELKDNKVVG
RNVVDFVHNDND
30 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 29 , which is depicted in the following (32):
(32)
ATGAAAGCTGCTGTTATTAATGATCCAGTAGACGGTTTTGTTACTGTTAAAGATGTTCAA
CTTCGGGATTTGAAGCCCGGTGAAGCTTTAGTTGACATGGAATATTGTGGTCTTTGTCAC
ACTGATCTACACGTTGCTGCTGGGGACTTTGGTAAGAAGCCCGGTCGTATTATCGGTCAC
GAAGGGGTTGGTCGTGTATCTAAGGTTGCCCCTGGCGTTACTTCCTTGAAAGTTGGCGAC
CGTGTATCAATTGCATGGTTCTTCAAGGGCTGTGGACACTGTGAATATTGTTTAACTGGT
CGTGAAACTCTTTGTCGGAACGTTCTTAATGCGGGTTACACTGCTGACGGTGCAATGGCT
GAACAATGTATCGTACCAGCTGACTACGCTGTTAAGGTTCCAGAAGGTCTTGATCCTGTT
GAAGCTACTTCATTAACTTGTGCTGGTGTTACGATGTACAAGGCATTAAAGGTTGCTGAC
ATCAAGCCAGGTCAATGGGTATCAATCGTTGGTGCTGGTGGTTTAGGTAACTTGGGTATT
CAACTTGCTCACAACGTATTTGGTGCTCATGTTATCGCTGTTGATGGTAATCCTGATAAG
CTTGAAGCCGCTAAGAAGAATGGTGCTGAAATTTTAATTAACCGTCATGACGGTGATGTT
GATAAGCAAATTCAAGAAAAGGTTGGCGGTGTTCACGCTGCTGTAGTAACAGCTGTTTCT
GCCTCTGCATTCGACCAAGCAGTTGATTCACTTCGCCCAGATGGTAAGCTTGTTGCCGTT
GCGCTTCCACAAGGTGACATGAAGCTTAACATTGCTAAGACTGTTCTTGATGGTATCATT
GTTGCTGGTTCATTAGTTGGTACCCGTCAAGACTTAGCTGAATGTTTCCAATTTGGTGCA
GAAGGTAAGGTTCACCCAATTGTTAAGACTCGTAAGTTAAGCGAAATTAATGATATGATC
CAAGAACTTAAGGATAACAAGGTTGTTGGTCGGAATGTTGTTGATTTTGTTCACAACGAT
AACGACTAA
31 . A glycerol-degrading enzyme depicted in the following amino acid sequence (17) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (17):
(17)
MEKRENAIPKTMKAWAVTTPGPIDGKESPI
EFTEKPVPTPKRGEVLVKVITCGVCHTDLH
VTEGDLPVHHEHVTPGHEIVGKVVGFGPET
QRFKFGERIGIPWFRHACGVCKFCRSGHEN
LCPHSLYTGWDHDGGYAEYVTVPEGFAYRL
PEKFDSLEAAPLLCAGIIGYRAFERANVPA
GGRLGLYGFGGSAHITAQIALAQGIEVHVF
TRGEDAKKFALELGCASVQGSYDPAPVPLD
SSIIFAPVGDMVLPALASLVPGGTLALAGI
HMTDIPTMNYQKEIFHEKTLTSVESNTRRD
GEEFLTLADRLNIHPEVHEYPLAKADEALR
YVKHGDIKGACVLRVSED
32 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 31 , which is depicted in the following (33):
(33)
ATGGAAAAACGCGAAAATGCTATTCCGAAAACAATGAAGGCTTGGGCAGTCACAACTCCT
GGGCCGATTGATGGTAAGGAATCACCAATCGAATTTACCGAAAAGCCTGTGCCGACTCCT
AAACGGGGAGAAGTCCTTGTTAAGGTAATAACGTGTGGAGTATGTCATACGGACTTGCAC
GTGACTGAAGGAGACTTGCCGGTTCACCACGAACACGTTACTCCTGGTCATGAAATTGTT
GGTAAAGTTGTCGGCTTTGGACCAGAGACACAACGATTTAAGTTTGGTGAGCGAATTGGG
ATTCCATGGTTTCGGCATGCTTGTGGTGTATGCAAGTTTTGCCGATCAGGTCATGAGAAT
CTCTGTCCTCATTCACTTTATACCGGTTGGGATCATGATGGCGGTTATGCAGAATATGTC
ACAGTTCCAGAAGGATTTGCATATCGGCTTCCAGAAAAGTTTGATTCCCTAGAGGCAGCT
CCGTTATTATGTGCAGGGATTATTGGTTATCGGGCCTTTGAACGTGCCAATGTTCCGGCT
GGCGGTCGCCTAGGATTATATGGCTTCGGTGGTTCAGCTCATATTACAGCTCAAATTGCA
CTTGCTCAGGGAATTGAAGTGCATGTCTTTACGCGTGGTGAGGATGCCAAGAAATTCGCC
CTAGAATTAGGTTGTGCTTCTGTTCAGGGCTCCTATGACCCAGCACCAGTTCCTTTGGAT
TCATCAATCATTTTTGCGCCGGTTGGTGATATGGTCTTGCCGGCTTTAGCTAGTTTAGTT
CCAGGGGGGACATTAGCATTAGCCGGTATTCATATGACTGATATTCCAACAATGAATTAC
CAAAAAGAAATATTCCACGAAAAGACATTAACGAGTGTTGAGAGTAATACTCGTCGTGAT
GGGGAAGAATTCTTAACATTAGCTGATCGTCTTAATATCCATCCTGAAGTCCACGAATAT
CCCCTAGCAAAGGCTGACGAAGCATTACGCTATGTTAAGCACGGTGATATTAAGGGAGCT
TGTGTATTACGTGTTAGTGAGGACTAA
33 . A glycerol-degrading enzyme depicted in the following amino acid sequence (18) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (18):
(18)
MQIKAALATKPNADLEIQTVELDEPKENEV
LIKIASTGFCHTDIVGRSGATTPLPVVLGH
EGAGVVQKVGANVTDVKPGDHVVLSFSYCG
HCYNCTHNHQGLCENFNQLNFEGKTYDGTH
RLHLDDGTPVSVFFGQSSFATYVTANVHNI
VKVDQDVDLNLLGPLGCGMQTGAGTVLNYI
KPAPEDAIAVFGAGAVGLAAIMAAKIAGVK
HIIAINRNGNHLDLAKELGATETINNTAED
PVKAIKEIVPRGVTYAIDTTGNTGVIKSAI
DSLATAGECVLLGVGGDITLDLMNDILSES
KKISGVVEGDSNPQEFIPQLVKYYKQSKFP
LDKLVKYYDFADINQVIADSTNGKVIKPII
KIDPELAKLPLTNDGSNVQKMVAEAGLADQ
ITIDSAGTSNIAEGSPADSRTKAILDKYHI
KDDGMIARQLQDRDYYDADYIIAMDQMNVR
DAKDMAPAGLENKVHGIFEATPGKENCYIV
DPWITH
34 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 33 , which is depicted in the following (34):
(34)
ATGCAAATTAAAGCTGCTCTTGCAACCAAACCTAACGCTGATTTAGAGATTCAAACCGTC
GAATTGGATGAACCAAAAGAAAATGAAGTATTAATAAAAATTGCTTCAACAGGTTTTTGT
CATACAGATATTGTTGGTCGAAGCGGTGCCACTACCCCTCTCCCCGTTGTCCTCGGGCAT
GAAGGTGCGGGCGTCGTCCAAAAAGTAGGAGCTAACGTTACGGACGTTAAACCCGGCGAC
CATGTTGTTCTATCATTTAGCTACTGTGGCCATTGCTATAACTGTACTCATAATCATCAA
GGCTTATGCGAAAACTTCAATCAGCTAAACTTTGAAGGAAAAACCTATGATGGTACTCAC
CGCCTGCACTTAGATGATGGCACGCCAGTCAGTGTCTTTTTTGGTCAGTCTTCCTTTGCG
ACCTATGTAACAGCCAATGTCCATAATATTGTTAAAGTTGATCAAGATGTTGATCTTAAC
TTATTAGGGCCACTCGGTTGTGGAATGCAAACAGGTGCTGGAACCGTTCTAAATTATATT
AAACCTGCTCCTGAAGATGCAATTGCCGTTTTCGGTGCTGGTGCTGTTGGCTTAGCCGCA
ATTATGGCTGCTAAAATTGCTGGAGTTAAACATATTATTGCGATTAATCGTAACGGTAAC
CACCTTGACCTGGCGAAGGAATTGGGCGCTACTGAAACGATTAATAATACGGCTGAAGAT
CCCGTCAAAGCAATTAAAGAAATCGTTCCGCGTGGTGTAACTTATGCAATCGATACTACC
GGAAACACCGGTGTAATTAAATCAGCAATTGATAGTCTTGCCACCGCTGGAGAATGTGTC
CTCTTAGGAGTTGGCGGCGATATTACCTTAGACTTAATGAATGATATCTTATCAGAATCT
AAGAAAATCTCTGGGGTTGTCGAAGGAGATAGCAATCCCCAAGAGTTTATTCCTCAACTA
GTTAAGTACTACAAGCAAAGCAAGTTCCCCCTTGATAAGCTTGTTAAGTACTACGATTTT
GCTGATATTAACCAAGTTATCGCTGACTCAACAAACGGAAAGGTTATTAAGCCAATCATC
AAAATTGATCCTGAATTAGCTAAATAATTGCCGCTCACCAATGACGGAAGCAATGTTCAA
AAAATGGTTGCAGAAGCTGGCCTTGCTGATCAAATTACTATTGATTCAGCCGGAACAAGT
AACATTGCAGAAGGTTCACCTGCTGATAGTCGAACAAAAGCCATTCTCGATAAATATCAC
ATTAAAGACGACGGAATGATTGCCCGTCAATTGCAGGACAGGGATTATTATGATGCCGAT
TATATTATCGCAATGGATCAGATGAATGTCCGGGACGCAAAAGATATGGCACCAGCTGGG
TTAGAAAATAAGGTTCATGGAATCTTTGAAGCTACCCCAGGAAAAGAAAATTGCTATATC
GTTGACCCCTGGATCACTCACTGA
35 . A glycerol-degrading enzyme depicted in the following amino acid sequence (19) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (19):
(19)
MKKAIFEKAGQMKIVDVDRPTIEKPDDVII
KVVRTCVCGSDLWNFRGINPVEKDSENSGH
EAIGIVEEVGEDITTVKPGDFVIAPFTHGC
GHCAACRAGFDGSCQSHNDNFSSGVQAQYV
RFQHGQWALVKVPGKPSDYSEGMLKSLLTL
ADVMATGYHAARVANVSDGDTVVVMGDGAV
GLCAIIAAKMRGAKKIISTSRHADRQALAK
EFGATDNVAERSDEAVQKIMELTNGAGADA
VLECVGTEQSTDTAMKVGRPGTIVGRVGLP
HTPKMDMTVLFYNNTIVGGGPASVTTYDKD
VLLKAVLDGDINPGKVFTKSFDLDQIQEAY
EAMDKREAIKSYIIMDGFERD
36 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 35 , which is depicted in the following (35):
(35)
ATGAAAAAAGCTATTTTTGAAAAGGCGGGTCAAATGAAGATTGTTGATGTTGACCGTCCA
ACAATTGAAAAGCCTGATGACGTAATTATTAAGGTAGTGCGGACCTGTGTTTGTGGTTCT
GACCTATGGAACTTCCGAGGAATTAATCCGGTTGAAAAAGATTCTGAAAACTCTGGCCAT
GAAGCAATTGGAATTGTTGAAGAAGTTGGTGAAGATATCACTACTGTCAAACCTGGGGAC
TTTGTGATTGCTCCATTTACTCATGGATGTGGGCACTGTGCTGCTTGTCGCGCGGGCTTC
GATGGTTCTTGCCAAAGTCACAACGATAACTTTAGCTCTGGTGTGCAAGCTCAATACGTT
CGGTTCCAACACGGTCAATGGGCGCTTGTTAAAGTTCCGGGCAAGCCAAGTGACTACAGT
GAAGGAATGCTTAAGTCCCTCTTAACCCTTGCTGATGTTATGGCTACTGGTTACCACGCT
GCACGAGTTGCTAACGTTAGTGATGGTGATACAGTTGTTGTAATGGGTGACGGTGCTGTT
GGCCTTTGTGCGATTATTGCTGCTAAGATGCGGGGCGCTAAGAAGATCATTTCTACTAGT
CGCCATGCTGACCGTCAAGCCCTTGCTAAGGAATTTGGTGCTACTGACAATGTTGCTGAA
CGTAGTGACGAAGCGGTTCAAAAGATCATGGAACTCACTAACGGTGCCGGTGCTGATGCT
GTCCTTGAATGCGTTGGTACTGAACAATCAACTGATACTGCCATGAAAGTTGGCCGTCCA
GGTACCATCGTTGGTCGGGTTGGCTTACCTCATACCCCAAAGATGGACATGACGGTGCTA
TTCTACAACAACACTATTGTCGGCGGTGGTCCAGCATCAGTAACCACTTACGACAAGGAC
GTATTGTTGAAGGCTGTTCTTGATGGTGACATTAACCCTGGTAAGGTCTTTACTAAGAGC
TTCGACCTTGACCAAATTCAAGAAGCTTATGAAGCAATGGATAAGCGTGAAGCAATCAAG
TCTTACATTATTATGGATGGCTTTGAACGCGATTAA
37 . A glycerol-degrading enzyme depicted in the following amino acid sequence (20) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (20):
(20)
MGRLDNKVAIITGGSKGIGAAVAKKFIEEG
AKVVLTARKMDEGQKVADQLGDNAIFIQQD
VARKGDWDRVIRQTVQVFGKLNIVVNNAGI
AEYADVEKTDAEIWDKTIAVNLTGTMWGTK
LGIEAMKNNGEKNSIINMSSIEGLIGDPDL
FAYNASKGGVRLLTKSAALDCARKGYDIRV
NTIHPGYISTPLVDNLVKDDPKAEGHLESL
HPLGRLGKPEEIANLALYLASDESSFSTGS
EFVADGGYTAQ
38 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 37 , which is depicted in the following (36):
(36)
ATGGGTCGTTTAGATAATAAAGTTGCAATTATTACTGGTGGTTCTAAAGGAATTGGAGCT
GCTGTCGCAAAAAAGTTTATCGAAGAAGGCGCAAAGGTTGTTTTAACCGCTCGGAAGATG
GATGAGGGACAAAAAGTCGCTGACCAACTAGGTGACAATGCGATCTTTATCCAACAAGAC
GTTGCTCGGAAAGGAGACTGGGACCGGGTAATCCGCCAAACTGTCCAAGTCTTTGGGAAG
CTCAATATTGTGGTTAACAATGCGGGAATTGCCGAATACGCCGATGTTGAGAAGACGGAC
GCTGAAATTTGGGATAAAACAATTGCCGTTAACCTTACCGGTACGATGTGGGGAACTAAG
CTCGGTATTGAAGCAATGAAGAACAACGGGGAAAAGAATTCAATCATCAATATGTCATCC
ATTGAAGGACTAATTGGTGATCCTGATCTCTTTGCATACAATGCTTCTAAGGGTGGTGTC
CGCCTCTTAACTAAGTCCGCTGCGCTTGATTGTGCCCGGAAAGGCTATGACATCCGTGTA
AATACAATTCATCCTGGTTATATCTCAACTCCACTAGTTGATAATTTGGTCAAGGATGAT
CCAAAAGCAGAAGGACACCTAGAAAGCCTTCATCCCCTTGGCCGTCTTGGAAAGCCAGAA
GAGATTGCTAACCTCGCTTTATACCTTGCTTCAGATGAATCAAGCTTTAGTACTGGTTCG
GAATTTGTCGCTGATGGTGGCTATACGGCTCAATAA
39 . A glycerol-degrading enzyme depicted in the following amino acid sequence (21) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (21):
(21)
MTNVPTVKLNNGVEMPTLGFEVFQVPDLSQ
AEQAVTDALEVGYRLIDTAAAYQNEEAVGK
AIKNSSVNREDVFVTSKLWVSDFNYKRAKA
GIDASLQKLGLDYMDLYLLHQPYGDTMGAW
RALQEAQKEGKIRAIGVSNFYADQLKDLEL
TMPVKPAVNQIEVNPWYQQDQEVKFAQSED
IRVEAWAPFAEGKHDIFTNEIIAEIAAKYG
KSNGQVILRWLLQRGITVIPKSVHKNRMEE
NIDVFDFELSNDDMKKIASLNKKESQFFDH
RDPVTIEQIFGSSLKMVQDDEK
40 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 39 , which is depicted in the following (37):
(37)
ATGACAAATGTACCAACAGTAAAATTAAATAACGGAGTAGAAATGCCAACCCTTGGATTT
GAAGTATTCCAAGTTCCAGACTTAAGCCAAGCTGAACAAGCAGTTACCGATGCTCTTGAA
GTCGGCTATCGTTTAATCGATACTGCTGCTGCTTACCAAAATGAAGAAGCAGTTGGAAAG
GCAATTAAGAATAGTAGTGTAAACCGTGAAGATGTCTTTGTAACTTCTAAGTTATGGGTG
TCTGATTTTAACTATAAGCGGGCTAAAGCAGGGATTGACGCTTCACTGCAAAAACTTGGC
CTTGATTACATGGATCTTTACCTTCTCCATCAACCATATGGCGATACAATGGGGGCTTGG
CGAGCATTACAAGAAGCACAGAAAGAAGGTAAGATTCGCGCAATCGGTGTATCGAACTTC
TACGCTGATCAACTAAAGGATCTTGAATTAACAATGCCTGTTAAGCCAGCGGTCAACCAA
ATTGAAGTTAACCCTTGGTACCAGCAAGATCAAGAGGTTAAGTTTGCGCAAAGTGAAGAT
ATTCGTGTTGAAGCATGGGCACCATTTGCGGAAGGTAAGCATGATATTTTTACCAACGAA
ATAATTGCGGAAATTGCTGCCAAGTATGGCAAGAGCAATGGTCAAGTAATTCTTCGCTGG
CTTTTACAACGGGGTATTACTGTCATTCCAAAGTCAGTCCACAAGAACCGGATGGAAGAA
AATATCGATGTCTTTGATTTTGAACTTTCCAATGATGATATGAAAAAGATAGCTAGTCTT
AACAAGAAGGAAAGCCAATTCTTTGACCACCGTGATCCGGTTACGATTGAACAAATCTTT
GGCTCCAGCTTAAAGATGGTTCAAGATGACGAAAAATAA
41 . A glycerol-degrading enzyme depicted in the following amino acid sequence (22) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (22):
(22)
MILDETITLNSGVKIPKFALGTWMIDDDQA
AEAVRNAIKMGYRHIDTAQAYDNERGVGEG
VRTAGIDRDKIFVTSKIAAEHKDYDVTKKS
IDETLEKMGLDYIDMMLIHSPQPWKEVNQS
DNRYLEGNLAAWRAMEDAVNEGKIRTIGVS
NFKKADLENIIKNSDTVPAVDQVLAHIGHT
PFNLLSFTHEHDIAVEAYSPVAHGAALDNP
VIEKMAKKYNVSVPQLCIRYDWQIGMIVLP
KTTNPEHMKENTEIDFEISEADMDLLRRVK
PLDYGDFDIYPVYGGKM
42 . A nucleotide sequence encoding the glycerol-degrading enzyme according to claim 41 , which is depicted in the following (38):
(38)
ATGATTTTAGATGAGACAATTACTCTTAATAGTGGTGTGAAAATTCCAAAGTTTGCATTA
GGAACCTGGATGATTGATGATGACCAAGCAGCCGAAGCAGTTCGGAATGCGATTAAGATG
GGATATCGGCACATCGATACAGCTCAGGCTTATGATAATGAGCGGGGAGTCGGTGAAGGT
GTACGAACAGCCGGTATTGATCGGGATAAAATCTTTGTTACTTCAAAGATCGCTGCTGAA
CACAAAGATTATGATGTAACTAAAAAGTCGATTGACGAGACTCTTGAAAAGATGGGTCTT
GATTATATCGACATGATGCTTATTCATAGTCCTCAACCATGGAAAGAAGTAAATCAATCT
GATAATCGTTACCTTGAAGGAAATCTCGCTGCTTGGCGAGCCATGGAAGATGCCGTTAAC
GAAGGTAAGATTCGAACAATTGGCGTTTCTAATTTCAAAAAAGCCGATCTTGAAAATATT
ATTAAGAATAGCGATACCGTTCCCGCTGTTGATCAAGTTTTAGCTCATATTGGTCATACT
CCATTCAATCTTTTATCATTTACTCATGAACATGACATTGCGGTTGAAGCATATTCACCA
GTTGCTCACGGCGCTGCTTTAGACAACCCCGTAATTGAAAAGATGGCTAAAAAGTACAAC
GTTTCAGTCCCACAATTGTGCATTCGGTATGATTGGCAAATAGGAATGATCGTCTTACCA
AAGACTACTAATCCAGAACACATGAAGGAAAACACTGAAATTGATTTTGAAATTTCTGAA
GCTGATATGGACCTATTGCGGCGAGTAAAGCCATTAGACTATGGCGATTTTGATATCTAC
CCTGTTTACGGTGGAAAAATGTAA
43 . An aldehyde dehydrogenase depicted in the following amino acid sequence (23) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (23):
(23)
MPANNKKQVEKKELTAEEKKQNAQKLVDDL
MTKSQAAFEKLRYYSQEQVDKICQAMALAA
EEHHMDLAVDAANETGRGVAEDKAIKNIYA
SEYIWNNIRHDKTVGIIEDNDEDQTIKIAD
PLGVIAGIVPVTNPTSTTIFKSIISAKTRN
TIIFSFHRQAMKSSIKTAKILQEAAEKAGA
PKNMIQWLPESTRENTTALLQHPNTATILA
TGGPSLVKAAYSSGNPALGVGPGNGPAYIE
KTANIERSVYDIVLSKTFDNGMICATENSV
VVDEEIYDKVKEEFQKWNCYFLKPNEIDKF
TDGFIDPDRHQVRGPIAGRSANAIADMCGI
KVPDNTKVIIAEYEGVGDKYPLSAEKLSPV
LTMYKATSHENAFDICAQLLHYGGEGHTAA
IHTLDDDLATKYGLEMRASRIIVNSPSGIG
GIGNIYNNMTPSLTLGTGSYGSNSISHNVT
DWDLLNIKTIAKRRENRQWVKIPPKVYFQR
NSLKELQDIPNINRAFIVTGPGMSKRGYVQ
RVIDQLRQRQNNTAFLVFDDVEEDPSTNTV
EKGVAMMNDFKPDTIIALGGGSPMDAAKAM
WMFYEHPETSWYGVMQKYLDIRKRAYQIKK
PTKSQLIGIPTTSGTGSEVTPFAVITDSKT
HVKYPLADYALTPNIAIVDSQFVETVPAKT
TAWTGLDVLCHATESYVSVMATDYTRGWSL
QTIKGVMENLPKSVQGDKLARRKMHDFSTM
AGMAFGQAFLGINHSLAHKMGGAFGLPHGL
LIAIAMPQVIRFNAKRPQKLALWPHYETYH
ATKDYADIARFIGLKGNTDEELAEAYAKKV
IELAHECGVKLSLKDNGVTREEFDKAVDDL
ARLAYEDQCTTTNPVEPLVSQLKELLERCY
DGTGVEEK
44 . A nucleotide sequence encoding the aldehyde dehydrogenase according to claim 43 , which is depicted in the following (39):
(39)
ATGCCTGCTAACAACAAGAAACAAGTTGAAAAGAAAGAATTAACTGCTGAAGAAAAAAAG
CAAAACGCCCAAAAGCTAGTTGACGATTTAATGACTAAGAGTCAAGCTGCTTTTGAAAAG
TTACGTTACTATTCACAAGAACAAGTTGACAAGATTTGTCAGGCAATGGCTCTCGCTGCC
GAAGAACACCACATGGACTTAGCTGTTGATGCTGCTAACGAAACTGGTCGTGGGGTTGCT
GAAGATAAGGCTATCAAGAACATCTACGCAAGTGAATACATTTGGAACAACATCCGTCAC
GATAAGACTGTTGGTATTATCGAAGACAATGATGAAGACCAAACTATCAAAATTGCTGAT
CCACTTGGTGTCATTGCCGGAATTGTTCCAGTTACTAACCCTACTTCAACAACGATCTTC
AAATCAATCATTAGTGCTAAGACACGGAATACAATCATCTTTTCTTTCCACCGTCAAGCA
ATGAAGTCATCTATCAAGACTGCAAAGATTCTCCAAGAAGCTGCTGAAAAAGCCGGTGCG
CCAAAGAACATGATTCAATGGCTCCCTGAAAGTACCCGCGAAAACACTACCGCATTACTC
CAACACCCTAATACTGCTACTATTTTAGCAACCGGTGGTCCTTCATTAGTTAAGGCTGCC
TACAGTTCTGGTAACCCTGCTCTTGGTGTTGGTCCTGGTAACGGTCCTGCTTACATCGAA
AAAACTGCCAACATCGAACGTTCTGTTTACGACATCGTTCTTTCTAAGACATTCGATAAC
GGTATGATTTGTGCCACTGAAAACTCAGTTGTTGTTGATGAAGAAATCTACGACAAGGTT
AAAGAAGAATTCCAAAAGTGGAACTGTTACTTCTTGAAGCCAAACGAAATTGATAAATTT
ACTGATGGCTTTATTGACCCAGATCGTCATCAAGTTCGTGGTCCAATCGCTGGTCGTTCA
GCTAATGCTATTGCTGACATGTGTGGTATTAAAGTACCTGACAACACTAAGGTTATCATT
GCTGAATACGAAGGTGTTGGTGACAAGTACCCACTTTCAGCTGAAAAGCTTTCACCAGTA
TTAACAATGTACAAGGCAACCTCTCACGAAAATGCCTTTGATATCTGTGCTCAATTATTA
CACTACGGTGGTGAAGGTCACACTGCTGCTATTCACACCCTTGATGATGATTTAGCTACT
AAGTACGGTCTTGAAATGCGTGCTTCACGGATCATTGTTAACTCCCCATCTGGTATTGGT
GGTATTGGTAACATCTACAACAACATGACTCCATCCCTTACTTTAGGTACTGGTTCATAC
GGTAGTAACTCAATTTCTCACAACGTTACTGATTGGGACCTCTTAAACATCAAAACAATT
GCAAAGCGGCGTGAAAACCGTCAATGGGTTAAGATTCCCCCAAAAGTATACTTTCAACGC
AACTCACTAAAAGAATTGCAAGATATTCCAAACATTAACCGGGCATTCATCGTTACTGGT
CCTGGAATGAGCAAGCGTGGTTACGTTCAACGTGTTATCGATCAATTGCGTCAACGCCAA
AACAACACTGCTTTCTTAGTATTTGATGACGTTGAAGAAGATCCATCAACAAACACTGTT
GAAAAAGGTGTTGCCATGATGAATGACTTCAAACCTGATACAATTATTGCTCTTGGTGGT
GGTTCACCAATGGATGCTGCTAAGGCTATGTGGATGTTCTATGAGCACCCAGAAACTTCA
TGGTATGGGGTTATGCAAAAGTACCTTGATATTCGGAAGCGTGCTTACCAAATCAAGAAG
CCTACTAAGTCTCAACTTATTGGTATCCCTACTACATCAGGTACTGGTTCAGAAGTTACT
CCATTTGCGGTTATTACCGATTCAAAAACTCATGTTAAGTACCCACTTGCTGACTACGCC
TTAACACCAAACATTGCAATCGTTGACTCACAATTCGTTGAAACTGTTCCAGCAAAAACT
ACTGCTTGGACTGGACTAGATGTTTTATGTCACGCTACTGAATCATATGTTTCTGTTATG
GCAACTGACTACACTCGTGGTTGGTCACTACAAACCATCAAGGGTGTTATGGAAAACCTT
CCTAAGTCAGTTCAAGGTGATAAGTTAGCTCGTCGTAAGATGCACGACTTCTCAACAATG
GCCGGGATGGCATTTGGTCAAGCCTTCTTAGGAATTAACCACTCCCTTGCCCACAAGATG
GGTGGAGCATTCGGTCTTCCTCACGGTTTGCTTATCGCTATTGCAATGCCACAAGTAATT
CGCTTTAACGCAAAACGTCCACAAAAGCTTGCTCTCTGGCCTCACTATGAGACTTACCAT
GCAACTAAGGACTACGCTGACATTGCACGGTTCATTGGTTTGAAAGGCAACACTGATGAA
GAATTAGCTGAAGCATATGCTAAGAAAGTTATCGAACTTGCTCACGAATGTGGTGTTAAG
CTTAGTCTTAAGGACAATGGTGTTACACGTGAAGAATTTGATAAGGCGGTTGACGATCTT
GCTCGCTTAGCTTACGAAGATCAATGTACTACTACTAACCCAGTTGAACCACTTGTTAGC
CAACTCAAGGAATTACTTGAACGTTGCTACGATGGTACTGGCGTTGAAGAAAAATAA
45 . An aldehyde dehydrogenase depicted in the following amino acid sequence (24) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (24):
(24)
MAYQSINPFTNQVEKTFENTTDEELEQTLT
TAHQLYLDWRKYNDLEERKRQILKLGQILR
ERRVEYATVMSKEMGKLISEAEGEVDLCAS
FCDYYAAHADEFLQPKIIATTSGRAKVLKQ
SLGILVAVEPWNFPFYQIARVFIPNFIAGN
PMILKDASNCPASAQAFNDAVKEAGAPAGS
LTNLFLSYDQVNKAIADKRVAGVCLTGSER
GGATVAKEAGANLKKSTLELGGNDAFIILD
DADWDLVEKVAPAARLYNAGQVCTSSKRFI
VLEKDYDRFLKMMKDAFSKVKMGDPLDPLT
TLAPLSSKKAKEKLQQQVATAVENGAKVYY
GNKPVDMEGQFFMPTILTDITPDNPIFDTE
MFGPVASVYKVSSEEEAIELANNSSYGLGN
TIFSNDSEHAERVAAKIETGMSWINAGWAS
LPELPFGGVKNSGYGRELSSYGIDEFTNKH
LIYEARQ
46 . A nucleotide sequence encoding the aldehyde dehydrogenase according to claim 45 , which is depicted in the following (40):
(40)
ATGGCATATCAAAGTATCAATCCATTTACGAACCAAGTAGAAAAAACGTTTGAAAATACA
ACTGATGAAGAATTAGAACAAACATTAACTACGGCGCATCAATTATATTTAGATTGGCGG
AAGTATAATGACCTTGAAGAACGGAAACGGCAAATTTTAAAGTTAGGTCAAATATTACGT
GAACGGCGTGTTGAATATGCGACAGTTATGAGTAAGGAAATGGGAAAATTAATTAGCGAA
GCAGAAGGCGAGGTTGACCTTTGTGCTTCTTTCTGTGATTATTATGCAGCCCATGCAGAT
GAATTTCTGCAACCAAAAATTATTGCGACAACGAGTGGACGCGCCAAAGTTTTGAAGCAA
TCATTAGGAATTTTAGTTGCAGTTGAACCTTGGAATTTCCCATTCTATCAAATTGCCCGG
GTATTTATTCCCAACTTTATTGCAGGAAACCCCATGATCTTGAAGGATGCGTCGAATTGT
CCAGCATCCGCCCAAGCATTTAACGATGCCGTTAAGGAAGCTGGTGCGCCAGCCGGCAGT
TTAACTAATTTATTCCTTTCATATGACCAAGTAAATAAGGCAATTGCTGATAAGCGGGTA
GCCGGCGTTTGTCTTACTGGTTCTGAACGTGGTGGTGCAACCGTTGCTAAAGAGGCTGGT
GCTAATTTGAAGAAGAGCACTTTGGAACTTGGTGGTAATGATGCCTTTATTATCTTAGAC
GATGCAGATTGGGATCTTGTCGAAAAAGTTGCCCCGGCAGCCCGTCTGTATAATGCTGGA
CAAGTATGTACATCATCAAAACGTTTTATTGTCCTTGAAAAGGATTATGATCGTTTCTTA
AAGATGATGAAAGATGCGTTCTCGAAAGTTAAAATGGGTGATCCCCTTGATCCATTAACA
ACTCTGGCACCATTATCATCTAAGAAAGCAAAAGAAAAGCTCCAACAGCAAGTCGCAACA
GCAGTAGAAAATGGGGCCAAAGTTTACTATGGTAATAAGCCGGTTGACATGGAAGGTCAA
TTCTTTATGCCAACGATCTTAACTGATATCACTCCAGATAACCCAATATTTGATACGGAA
ATGTTTGGGCCAGTGGCTTCGGTTTATAAGGTTAGTTCCGAAGAGGAAGCAATCGAACTG
GCTAATAATTCAAGCTATGGGTTAGGAAACACTATCTTTAGCAATGATTCCGAACATGCG
GAACGAGTAGCAGCGAAGATCGAAACTGGAATGAGTTGGATTAATGCCGGCTGGGCTTCA
TTACCAGAATTACCATTTGGTGGTGTTAAGAATTCAGGTTACGGTCGTGAACTCAGCAGT
TACGGAATTGATGAATTTACTAACAAACATCTAATTTACGAAGCACGACAATAA
47 . An aldehyde dehydrogenase depicted in the following amino acid sequence (25) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (25):
(25)
MQINDIESAVRKILAEELDNASSSSANVAA
TTDNGHRGIFTNVNDAIAAAKAAQEIYRDK
PIAVRQQVIDAIKEGFRPYIEKMAKDIKEE
TGMGTVEAKIAKLNNALYNTPGPEILEPVV
ENGDGGMVMYERLPYGVIGAVGPSTNPSET
VIANAIMMLAGGNTLYFGAHPGAKNVTRWT
IEKMNDFIADATGLHNLVVSIETPTIESVQ
QMMKHPDIAMLAVTGGPAVVHQAMTSGKKA
VGAGPGNPPAMVDATADIDLAAHNIITSAS
FDNDILCTAEKEVVAESSIKDELIRKMQDE
GAFVVNREQADKLADMCIQENGAPDRKFVG
KDATYILDQANIPYTGHPVEIICELPKEHP
LVMTEMLMPILPVVSCPTFDDVLKTAVEVE
KGNHHTATIHSNNLKHINNAAHRMQCSIFV
VNGPSYVGTGVADNGAHSGASALTIATPTG
EGTCTARTFTRRVRLNSPQGFSVRNWY
48 . A nucleotide sequence encoding the aldehyde dehydrogenase according to claim 47 , which is depicted in the following (41):
(41)
ATGCAGATTAATGATATTGAAAGTGCTGTACGCAAAATTCTTGCCGAAGAACTAGATAAT
GCCAGCTCTTCAAGTGCAAACGTTGCAGCTACTACTGATAATGGTCATCGCGGAATTTTC
ACTAATGTCAATGATGCAATTGCTGCTGCAAAAGCTGCTCAAGAAATATATCGGGATAAG
CCAATTGCTGTTCGCCAACAAGTGATTGATGCCATTAAGGAAGGATTCCGCCCATATATT
GAAAAAATGGCTAAAGATATCAAAGAAGAAACAGGAATGGGAACAGTAGAGGCCAAAATT
GCTAAGTTAAACAATGCCTTGTACAACACTCCTGGTCCCGAGATTCTTGAACCAGTTGTA
GAAAACGGTGACGGTGGGATGGTTATGTATGAACGGTTACCATATGGTGTTATTGGTGCG
GTTGGCCCAAGTACAAACCCTTCAGAAACTGTAATTGCTAATGCGATCATGATGCTTGCC
GGTGGTAATACTCTTTACTTTGGTGCTCACCCTGGCGCAAAGAATGTTACTCGCTGGACA
ATTGAAAAGATGAACGATTTTATTGCAGATGCAACAGGCCTTCATAATTTAGTTGTAAGT
ATTGAAACACCAACAATTGAATCAGTTCAACAAATGATGAAGCACCCCGACATTGCAATG
TTAGCAGTAACTGGTGGCCCAGCTGTTGTTCACCAAGCAATGACCAGTGGTAAGAAAGCG
GTTGGTGCTGGTCCTGGTAATCCTCCTGCAATGGTTGATGCTACTGCTGATATTGATTTA
GCTGCTCATAATATCATTACATCTGCTTCATTTGATAATGATATTTTATGTACTGCTGAA
AAGGAAGTAGTTGCAGAAAGTAGCATTAAAGATGAATTAATTCGTAAGATGCAAGATGAA
GGTGCCTTTGTAGTTAACCGTGAACAAGCCGATAAATTAGCTGATATGTGTATCCAAGAA
AATGGTGCTCCTGATCGTAAATTTGTTGGTAAGGATGCAACTTATATCTTAGACCAAGCT
AATATTCCTTACACAGGCCACCCAGTTGAAATTATTTGTGAACTTCCTAAGGAACATCCA
TTAGTAATGACTGAAATGTTAATGCCAATTTTACCAGTTGTTTCTTGTCCAACATTTGAT
GATGTTTTGAAGACTGCTGTTGAAGTTGAAAAAGGTAACCATCACACAGCTACTATTCAT
TCCAATAACCTTAAGCATATTAATAATGCTGCTCACCGGATGCAATGTTCAATCTTTGTT
GTTAATGGCCCATCCTATGTTGGTACAGGTGTTGCAGATAATGGAGCTCACTCAGGTGCT
TCAGCATTAACAATTGCTACGCCAACTGGTGAAGGAACATGTACTGCACGAACATTTACT
CGTCGGGTTCGTTTGAACTCACCACAAGGATTCTCAGTACGTAACTGGTATTAA
49 . A glycerate kinase depicted in the following amino acid sequence (26) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (26):
(26)
MKFVIAPDSFKGGLTAKEAANVMAEGIKRV
FPNAEYALVPMADGGEGTVQSLVDATNGQK
MIAKVHNPLNKLVNAEYGILGDGETAVIEM
AAASGLQFVNKETANPLITTTYGTGELIKD
ALDHNIKKIIIGIGGSATVDGGAGMAQALG
ARLLDADNHEIGLGGGELASLEQVDFGGLD
PRLKNVDIQIASDVTNPLTGKNGAAPVFGP
QKGADEEMVNILDKNLHHYARKIVAAGGPD
VEQTAGAGAAGGLGAGLIAFTGATMKRGVE
LVIEATQLQKKAVGADYVFTGEGGIDFQTK
FGKTPYGVAKATKEVAPTAPVIVLAGNIGK
GVNDLYSSTAIDAIFATPEGAKPLKTALAD
APIDIAQTAENVARLIKVSHVSN
50 . A nucleotide sequence encoding the glycerate kinase according to claim 49 , which is depicted in the following (42):
(42)
ATGAAATTTGTAATTGCTCCAGATTCATTTAAAGGCGGATTAACAGCAAAAGAAGCAGCA
AATGTGATGGCAGAAGGAATCAAAAGAGTGTTTCCGAATGCCGAGTATGCTTTAGTTCCA
ATGGCTGATGGAGGAGAGGGGACTGTTCAATCCTTAGTTGATGCGACTAACGGTCAAAAA
ATGATTGCTAAAGTCCACAACCCATTAAATAAATTAGTTAATGCTGAGTACGGAATATTA
GGTGATGGGGAAACGGCAGTGATTGAGATGGCGGCGGCAAGTGGCCTTCAATTTGTTAAT
AAGGAGACTGCGAACCCGCTTATTACAACTACATATGGTACCGGCGAGTTAATTAAGGAT
GCTCTTGACCATAACATTAAAAAAATAATTATTGGAATTGGTGGAAGTGCAACCGTTGAT
GGCGGAGCGGGGATGGCCCAAGCACTTGGAGCACGTTTATTGGATGCTGATAATCATGAA
ATTGGTTTAGGCGGTGGTGAGTTAGCAAGTTTAGAGCAAGTAGATTTTGGAGGATTAGAT
CCTCGCTTAAAAAATGTAGATATTCAGATTGCATCAGACGTAACCAACCCATTAACAGGA
AAAAATGGGGCAGCCCCAGTATTTGGCCCGCAAAAAGGAGCTGATGAAGAAATGGTGAAC
ATCTTGGACAAAAATCTTCATCATTATGCCCGAAAAATAGTTGCAGCTGGTGGGCCAGAC
GTTGAACAAACGGCAGGTGCAGGGGCAGCCGGTGGTTTAGGAGCCGGGTTGATAGCATTT
ACCGGTGCGACAATGAAGCGAGGAGTAGAATTAGTGATTGAAGCAACTCAACTACAAAAA
AAGGCAGTTGGCGCTGATTATGTTTTTACTGGTGAAGGAGGAATTGATTTCCAGACTAAA
TTTGGTAAAACGCCATATGGAGTCGCTAAGGCAACTAAAGAGGTGGCTCCAACTGCTCCG
GTAATTGTGTTGGCTGGAAATATTGGTAAAGGCGTAAATGATCTATATTCATCCACGGCC
ATTGATGCAATTTTTGCAACTCCTGAAGGGGCTAAACCATTAAAAACAGCATTAGCAGAT
GCACCTATTGATATTGCTCAAACAGCGGAAAACGTTGCACGTTTAATTAAAGTGAGTCAT
GTTAGTAATTAA
51 . A glycerol kinase depicted in the following amino acid sequence (27) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (27):
(27)
LSEQQYIMAIDQGTTSSRAIIFDHDGNKVA
ISQQEFPQYFPQPGWVEHDPLEIWDSVQSV
ISNVMIKSQIKPYKIAAIGITNQRETTVIW
DRHTGKPIYNAIVWQSKQTSDIAEQLIKDG
YKDMIHQKTGLVIDSYFAATKIKWILDHVP
GAREKAAKGDLMFGTIDTWLLWNLSGRRVH
ATDVTNASRTMLFNIHTLDWDQDILDLLDI
PQSLLPVVKPSSAIYGYTGDYHFYGVQIPI
AGIAGDQQAALFGQAAYDKGSIKNTYGTGA
FIVMNTGLKPTLSDNGLLTTIAYGLDGQTH
YALEGSIFVAGSAVQWLRDGLKMFDKASES
EQMAVDAKTTGGVYVVPAFTGLGAPYWDQE
VRGAMFGLTRGTERGHIIRATLEAIAYQTK
DVVDTMVKDTQLPLTALTVNGGASRNNFMM
QFQADILQTPIKRAAMEETTALGAAFLAGL
AVDFWEDQDELRKLSRIGDQFDPQMDPQKA
ADLYRGWQRAIAAAQFYGKD
52 . A nucleotide sequence encoding the glycerol kinase according to claim 51 , which is depicted in the following (43):
(43)
TTGAGTGAACAACAATATATCATGGCGATTGACCAGGGAACGACGAGCTCACGGGCGATT
ATCTTTGACCATGACGGAAATAAGGTTGCGATCAGTCAGCAGGAATTTCCCCAATACTTC
CCGCAGCCGGGGTGGGTTGAACATGATCCTCTAGAGATTTGGGATAGCGTTCAATCAGTG
ATTTCAAATGTAATGATTAAGTCCCAGATCAAGCCCTATAAGATTGCGGCAATTGGGATT
ACTAACCAACGGGAGACGACGGTTATTTGGGATCGCCATACCGGTAAGCCGATTTATAAC
GCAATTGTCTGGCAATCGAAGCAAACGAGCGACATCGCCGAACAATTGATTAAAGATGGT
TATAAGGATATGATCCACCAGAAGACTGGCTTGGTGATTGATTCGTATTTCGCGGCCACT
AAGATCAAGTGGATCCTTGACCATGTTCCTGGTGCCCGGGAAAAAGCAGCAAAGGGAGAC
TTGATGTTTGGGACTATCGATACTTGGTTACTATGGAATTTATCGGGACGGCGGGTCCAC
GCAACGGATGTGACCAATGCCAGCCGGACGATGCTTTTTAATATCCATACCCTCGACTGG
GATCAAGATATCCTTGACCTGCTTGATATTCCCCAGTCGCTTTTGCCAGTAGTAAAGCCA
AGTTCAGCCATTTACGGTTATACTGGCGACTACCACTTCTATGGGGTGCAGATTCCAATT
GCCGGGATTGCAGGTGACCAACAAGCAGCCCTCTTTGGTCAAGCAGCCTATGATAAAGGT
TCAATCAAGAACACCTATGGGACTGGAGCCTTCATCGTCATGAATACGGGACTAAAACCC
ACGCTTTCGGATAACGGCTTGTTGACGACGATTGCGTATGGCCTGGACGGGCAAACTCAT
TACGCGCTTGAAGGAAGTATCTTTGTGGCCGGTTCTGCCGTTCAATGGTTGCGGGATGGT
CTCAAGATGTTTGATAAGGCAAGCGAGTCCGAACAAATGGCTGTCGATGCCAAGACAACT
GGCGGCGTTTATGTCGTCCCCGCCTTTACAGGATTAGGCGCACCGTACTGGGATCAAGAA
GTGCGGGGCGCAATGTTTGGCCTTACCCGTGGAACTGAACGGGGACATATCATCCGTGCA
ACTTTGGAAGCCATTGCCTACCAGACCAAAGATGTTGTCGATACGATGGTCAAGGACACC
CAATTACCACTAACAGCACTAACGGTTAACGGGGGCGCTTCACGGAACAACTTCATGATG
CAGTTCCAGGCCGATATCTTACAAACGCCAATCAAGCGGGCAGCAATGGAAGAGACAACC
GCGCTGGGAGCAGCCTTTCTCGCTGGATTGGCCGTTGATTTCTGGGAAGACCAGGATGAG
TTACGGAAGCTATCACGGATTGGCGACCAGTTTGATCCACAAATGGATCCGCAAAAGGCA
GCTGACTTGTATCGGGGATGGCAACGGGCCATTGCAGCTGCGCAGTTTTATGGCAAAGAT
TAA
53 . A glycerol-3-phosphate dehydrogenase depicted in the following amino acid sequence (28) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (28):
(28)
MAEKIAVLGAGSWGSVLANMLTENGHDVTL
WSRNEEQVKQLNTEHTNPRYMKDFVYSTNL
TATTDMKKAVKGASVVLIVIPTKGLREVAK
QLNAILTELHQKPLVIHATKGLEQNTYKRP
SEMLSEDISPENRQAIVVLSGPSHAEDVAI
KDMTAVTAACEDLASAKKAQKLFSNSYFRV
YTNDDVIGAEFGAALKNIIAIGAGAIQGLG
YHDNARAALITRGLAEIRRLGVAFGANPMT
FIGLSGVGDLVVTATSKNSRNWRAGYQLGQ
GKKLQDVIDNMGMVIEGVYTTKAAYELSRK
RQVQMPITEALYRVLYEGEDIKTAISQLMD
RDLTSENE
54 . A nucleotide sequence encoding the glycerol-3-phosphate dehydrogenase according to claim 53 , which is depicted in the following (44):
(44)
ATGGCAGAAAAAATTGCTGTTTTAGGTGCTGGTTCGTGGGGCAGTGTTTTAGCAAACATG
CTTACAGAAAATGGCCACGATGTAACATTATGGTCTCGTAATGAGGAACAAGTTAAGCAA
TTAAATACTGAACATACAAATCCTCGCTATATGAAAGATTTTGTTTATTCTACTAACTTA
ACAGCAACAACGGACATGAAAAAAGCTGTTAAGGGTGCCAGTGTGGTCCTGATTGTAATT
CCAACAAAGGGTCTTCGTGAAGTTGCTAAGCAATTAAATGCAATTTTGACTGAATTACAT
CAAAAACCGCTAGTTATTCACGCAACGAAAGGCTTAGAACAAAATACTTATAAGCGGCCA
TCGGAAATGCTTAGCGAAGATATTTCTCCTGAAAACCGTCAGGCAATTGTTGTTTTATCA
GGTCCGAGTCATGCTGAAGATGTGGCGATTAAAGATATGACAGCTGTAACCGCAGCTTGT
GAGGACCTGGCCAGTGCTAAAAAGGCGCAGAAGTTATTTAGTAATTCTTATTTCCGTGTG
TACACTAATGACGATGTAATTGGTGCCGAATTTGGCGCAGCCTTAAAGAACATTATTGCA
ATTGGTGCTGGAGCTATTCAGGGACTTGGTTATCATGATAATGCTCGGGCAGCGTTAATT
ACTCGTGGACTTGCAGAAATTCGCCGATTGGGAGTTGCTTTTGGTGCCAACCCGATGACT
TTTATTGGTCTTTCTGGGGTTGGTGACCTTGTTGTTACTGCTACCAGTAAAAATTCTCGA
AATTGGCGTGCTGGCTATCAATTGGGGCAAGGAAAAAAGCTTCAAGATGTAATTGATAAT
ATGGGAATGGTTATCGAAGGTGTCTATACTACCAAAGCCGCTTATGAATTAAGTCGTAAA
CGACAAGTACAGATGCCAATTACCGAAGCTCTTTACCGTGTTTTGTATGAAGGCGAAGAT
ATTAAAACTGCAATTTCTCAATTAATGGACCGAGATCTTACTTCAGAAAACGAATAA
55 . A triosephosphate isomerase depicted in the following amino acid sequence (29) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (29):
(29)
MRKPFIAANWKMHKNVQESVEFVDAIKGKL
PDPQEVEVGIAAQAFALPSMVQAADDSGLK
IIAQNAAAEYSGAFTGEISLRGLADAGVSY
VMLGHIERRHLFHEDNELVNRKVLAALQMG
VTPIICTDETMVQKEVNGEIHYVFQQLMSV
LRGVSLDQIKNVVVSYEPSWAVGYGQHANP
VLAEEGCRQIRRTIADNYTYEIADKIRILY
GGSVNPDNIGMIMNKPDVDGVLIGRASLDV
DNFLRMVNYLKNDQEK
56 . A nucleotide sequence encoding the triosephosphate isomerase according to claim 55 , which is depicted in the following (45):
(45)
ATGCGCAAACCCTTTATTGCTGCTAATTGGAAGATGCATAAGAATGTCCAAGAATCGGTT
GAATTTGTGGATGCAATTAAAGGAAAGCTACCAGATCCGCAAGAAGTTGAAGTCGGAATT
GCAGCCCAAGCTTTTGCATTACCCAGTATGGTTCAAGCCGCTGATGATTCAGGATTAAAG
ATAATCGCGCAAAACGCGGCGGCTGAATATTCGGGAGCTTTCACTGGTGAAATTAGCTTA
CGAGGTTTAGCTGACGCCGGTGTTTCATATGTAATGTTAGGACATATTGAACGGCGCCAT
TTATTCCACGAGGATAATGAGTTGGTTAATCGGAAAGTGTTGGCAGCCCTTCAAATGGGA
GTTACCCCGATAATTTGTACGGATGAAACGATGGTCCAGAAAGAAGTTAATGGTGAAATT
CACTACGTTTTCCAGCAATTGATGAGCGTATTGAGGGGCGTTTCTCTTGATCAAATTAAA
AATGTAGTTGTTTCCTATGAACCAAGTTGGGCAGTTGGATATGGTCAGCATGCTAATCCA
GTTCTTGCTGAAGAAGGATGCCGTCAAATTCGGCGAACGATTGCTGATAACTACACTTAT
GAGATTGCTGATAAGATCAGGATTCTTTATGGGGGCAGTGTCAATCCAGATAATATCGGA
ATGATTATGAACAAGCCAGATGTAGATGGGGTATTAATCGGTCGGGCAAGTTTAGATGTT
GATAATTTTTTGCGAATGGTCAATTATTTAAAAAATGATCAAGAAAAATAA
57 . A triosephosphate isomerase depicted in the following amino acid sequence (30) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (30):
(30)
MRKPFIIANWKMNKNVHESVAFVKAIKEKL
PADKEIGIAAQAVSLYNMKKVASSSNLQII
AQNASAELEGPYTGEISMRSLADAGVTYVM
LGHLERRRLFNESNDSINQKVLAALNAGII
PIICTDEEMVQTEVNGQIHYVFRQLKSVLK
GVPANKLSQIVISYEPSWAVGSTHQANPDI
AEEGCQAIRQSLVEMYGNEIGEQVRILYGG
SVNPENIGQIMSKPNVDGALIGRASLEIES
FLQMINYIELASKQKLQVI
58 . A nucleotide sequence encoding the triosephosphate isomerase according to claim 57 , which is depicted in the following (46):
(46)
ATGCGCAAACCGTTTATTATTGCGAACTGGAAAATGAATAAAAACGTTCATGAATCTGTT
GCGTTTGTTAAAGCAATTAAAGAAAAGCTCCCGGCAGATAAAGAAATTGGGATCGCCGCG
CAAGCAGTTTCGCTATATAACATGAAAAAAGTGGCGAGCTCTTCCAACTTACAAATTATT
GCTCAAAATGCATCTGCTGAGTTAGAGGGACCATATACTGGAGAAATTAGCATGCGAAGT
TTAGCAGATGCGGGCGTGACATACGTGATGCTAGGCCATTTAGAGCGCCGACGCCTTTTT
AACGAGAGTAATGATTCAATTAATCAAAAAGTTTTAGCAGCCCTCAATGCTGGTATTATT
CCAATCATTTGTACGGATGAAGAGATGGTCCAAACAGAAGTTAACGGACAAATTCATTAT
GTATTTCGCCAACTAAAAAGCGTCCTTAAAGGGGTACCAGCTAATAAACTATCACAGATT
GTTATTTCGTATGAACCAAGTTGGGCCGTTGGGAGCACGCATCAAGCAAATCCAGACATT
GCGGAAGAGGGATGTCAGGCAATTCGTCAAAGCCTGGTTGAAATGTATGGTAATGAGATT
GGCGAGCAAGTCCGAATACTCTATGGTGGCAGCGTTAATCCCGAGAACATTGGTCAAATT
ATGAGTAAACCAAATGTTGATGGGGCGCTAATCGGTCGCGCAAGTCTCGAGATTGAAAGT
TTCTTACAAATGATTAATTATATCGAATTAGCGAGCAAGCAGAAGTTACAGGTAATTTAG
59 . A triosephosphate isomerase depicted in the following amino acid sequence (31) or an amino acid sequence having deletion, substitution or addition of one or more amino acids in the amino acid sequence (31):
(31)
MRVPIIAGNWKMHKDVQEAVSFIEKVKNQL
PPADQLETAIAAPTLCLVPMVKAAEESPLK
IMAENCYYKNEGAYTGETSPYALYQAGIHH
VILGHSERRTYFNETDELINKKVKAALVNG
LCPIVCCDDTMRRRVAGKKVHWVVSRILAD
LHGLTNDEICHVTVAYEPSWAIGTGESADP
EQAAEGCYLIRQTISDMYGDEVANNVRILY
GGSVTTSNINALMAKNDIDGVLVGAASLNP
ETFLQLVHH
60 . A nucleotide sequence encoding the triosephosphate isomerase according to claim 59 , which is depicted in the following (47):
(47)
ATGAGAGTACCGATTATTGCTGGTAATTGGAAAATGCATAAGGATGTACAAGAAGCTGTC
TCTTTTATCGAAAAAGTAAAAAATCAGCTTCCGCCTGCCGACCAACTTGAAACAGCAATT
GCTGCTCCTACTCTTTGTTTAGTACCAATGGTTAAAGCAGCTGAAGAATCCCCGTTAAAA
ATAATGGCAGAAAACTGCTACTATAAGAATGAGGGAGCTTATACTGGTGAAACAAGTCCA
TATGCTTTATACCAAGCAGGAATCCATCATGTGATTTTAGGCCATTCTGAACGCCGAACT
TACTTTAATGAAACTGATGAATTAATTAATAAAAAAGTGAAGGCAGCATTAGTAAATGGG
TTATGTCCGATTGTTTGTTGTGATGATACTATGCGTCGACGAGTTGCTGGAAAGAAAGTT
CATTGGGTGGTGAGCCGAATTCTCGCTGACCTTCATGGATTGACCAATGACGAAATTTGT
CATGTTACGGTTGCTTATGAACCAAGTTGGGCGATTGGAACAGGCGAGAGTGCTGATCCA
GAACAAGCGGCGGAAGGTTGTTACCTTATTCGGCAAACGATTAGTGATATGTATGGCGAT
GAAGTTGCAAATAACGTTCGAATTCTCTATGGCGGAAGTGTGACAACTTCTAATATCAAT
GCACTAATGGCAAAAAATGATATTGATGGTGTTTTAGTCGGAGCGGCGAGCTTAAATCCA
GAAACATTTTTACAATTAGTTCACCATTAGJoin the waitlist — get patent alerts
Track US2010216212A1 — get alerts on status changes and closely related new filings.
We store only your email — no account needed. See our privacy policy.