Method of identification and quantification of proteins, isoforms of the angiotensin i converting enzyme, molecular genetic marker based on said proteins, use of mentioned marker, analytical method for diagnosis, risk stratification, therapeutical decision in carriers of arterial hypertension and renal lesion and kit for using in the diagnosie

Abstract

A method of detecting a predisposition for the development of a kidney lesion in an individual including detecting a presence of at least three angiotensin converting enzyme isoforms in an aliquot of fresh or concentrated biological fluids, cells or tissues obtained from the individual; and quantifying the presence of the at least three antiotension converting enzyme isoforms.

1 .- 22 . (canceled) 
   
   
       23 . A method of detecting a predisposition for the development of a kidney lesion in an individual, comprising:
 detecting a presence of at least three angiotensin converting enzyme isoforms in an aliquot of fresh or concentrated biological fluids, cells or tissues obtained from the individual; and   quantifying the presence of the at least three antiotension converting enzyme isoforms.   
   
   
       24 . The method of claim  37 , wherein the aliquot of fresh or concentrated biological fluids, cells or tissues is urine. 
   
   
       25 . The method of claim  37 , wherein said detecting step comprises an immunoprecipitation method. 
   
   
       26 . The method of claim  40 , wherein the immunoprecipitation method is Western blotting. 
   
   
       27 . The method of claim  37 , wherein said detecting step comprises a mass detection methodology. 
   
   
       28 . The method of claim  42 , wherein the mass detection methodology comprises mass spectroscopy. 
   
   
       29 . The method of claim  43 , wherein the mass detection methodology is used in combination with a chromatographic separation. 
   
   
       30 . The method of claim  44 , wherein the mass detection methodology comprises mass spectroscopy.