Nucleotide sequences regulating gene expression and constructs and methods utilizing same

Abstract

Novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in plants are provided

1 . An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 136, 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 56, 61, 66, 71, 76, 81, 86, 91, 96, 101, 106, 11, 116, 121, 126, 131, 141, 146, 151, 156, 161, 166, 171, 176, 181, 186, 191, 196, 201, 202, 203, 210 and 213 wherein the isolated polynucleotide is capable of regulating expression of at least one heterologous polynucleotide sequence operably linked thereto. 
     
     
         2 . An isolated polynucleotide consisting of the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 136, 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 56, 61, 66, 71, 76, 81, 86, 91, 96, 101, 106, 11, 116, 121, 126, 131, 141, 146, 151, 156, 161, 166, 171, 176, 181, 186, 191, 196, 201, 202, 203, 210 and 213. 
     
     
         3 . A nucleic acid construct, comprising the isolated polynucleotide of  claim 1 . 
     
     
         4 . A nucleic acid construct, comprising the isolated polynucleotide of  claim 2 . 
     
     
         5 . The nucleic acid construct of  claim 3 , further comprising at least one heterologous polynucleotide operably linked to the isolated polynucleotide. 
     
     
         6 . The nucleic acid construct of  claim 4 , further comprising at least one heterologous polynucleotide operably linked to the isolated polynucleotide. 
     
     
         7 . The nucleic acid construct of  claim 5 , wherein said heterologous polynucleotide is an expressed nucleic acid sequence. 
     
     
         8 . The nucleic acid construct of  claim 5 , wherein said heterologous polynucleotide is a reporter gene. 
     
     
         9 . The isolated polynucleotide of  claim 1 , wherein said regulating is effected in a constitutive manner. 
     
     
         10 . The isolated polynucleotide of  claim 1 , wherein said regulating is effected in an inductive manner. 
     
     
         11 . The isolated polynucleotide of  claim 1 , wherein said regulating is effected in a tissue specific manner. 
     
     
         12 . The isolated polynucleotide of  claim 1 , wherein said regulating is effected in a developmental stage specific manner. 
     
     
         13 . The nucleic acid contrast of  claim 5 , wherein said at least one heterologous polynucleotide is at least two heterologous polynucleotides each being operably linked to an end of the isolated polynucleotide such that said two heterologous polynucleotides flank the isolated polynucleotide. 
     
     
         14 . A transgenic cell comprising the isolated polynucleotide of  claim 1 . 
     
     
         15 . A transgenic cell comprising the nucleic acid construct of  claim 3 . 
     
     
         16 . A transgenic non-human organism comprising the isolated polynucleotide of  claim 1 . 
     
     
         17 . A transgenic non-human organism comprising the nucleic acid construct of  claim 3 . 
     
     
         18 . A transgenic plant comprising the isolated polynucleotide of  claim 1 . 
     
     
         19 . A transgenic plant comprising the nucleic acid construct of  claim 3 . 
     
     
         20 . A method of producing a transgenic plant, comprising transforming a plant with the polynucleotide of  claim 1 . 
     
     
         21 . A method of producing a transgenic plant, comprising transforming a plant with the nucleic acid construct of  claim 3 . 
     
     
         22 . A method of producing a transgenic plant, comprising transforming a plant with the nucleic acid construct of  claim 5 . 
     
     
         23 . A method of producing a transgenic plant, comprising transforming a plant with the nucleic acid construct of  claim 7 . 
     
     
         24 . A method of expressing a polypeptide of interest in a cell comprising transforming the cell with a nucleic acid construct including a polynucleotide sequence encoding the polypeptide of interest operably linked to a regulatory nucleic acid sequence selected from the group consisting of SEQ ID NOs: 136, 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 56, 61, 66, 71, 76, 81, 86, 91, 96, 101, 106, 11, 116, 121, 126, 131, 141, 146, 151, 156, 161, 166, 171, 176, 181, 186, 191, 196, 201, 202, 203, 210 and 213 thereby expressing the polypeptide of interest in the cell. 
     
     
         25 . A method of co-expressing two polypeptides of interest in a cell comprising transforming the cell with a nucleic acid construct including two polynucleotide sequences encoding the two polypeptides of interest operably linked to a regulatory nucleic acid sequence selected from the group consisting of SEQ ID NOS: 136, 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 56, 61, 66, 71, 76, 81, 86, 91, 96, 101, 106, 11, 116, 121, 126, 131, 141, 146, 151, 156, 161, 166, 171, 176, 181, 186, 191, 196, 201, 202, 203, 210 and 213 such that said two polynucleotide sequences flank said regulatory nucleic acid sequence, thereby expressing the two polypeptides of interest in the cell.