Host Cells Used For Production of Recombinant Protein

Abstract

It is an object of the present invention to provide host cells, which are able to proliferate in a serum free medium, which are able to synthesize and secrete a large amount of protein of interest, and which are able to produce a glycoprotein having a sugar chain structure. The present invention provides an established cell line, which is established from distal tubular epithelial cells derived from a mammal.

1 . An established cell line, which is established from distal tubular epithelial cells derived from a mammal. 
     
     
         2 . The established cell line according to  claim 1 , which is used as a host cell for production of a recombinant protein. 
     
     
         3 . The established cell line according to  claim 1 , which is derived from a cat. 
     
     
         4 . The established cell line according to  claim 1 , which has a gene encoding a foreign immortality-imparting factor. 
     
     
         5 . The established cell line according to  claim 1 , which has a foreign selective marker gene. 
     
     
         6 . The established cell line according to  claim 1 , which is able to proliferate in a serum free medium. 
     
     
         7 . The established cell line according to  claim 1 , which is a cell having an accession No. FERM BP-10643. 
     
     
         8 . A transformant, which is obtained by introducing a gene encoding a protein of interest to be expressed into the established cell line of  claim 1 . 
     
     
         9 . A method for producing a recombinant protein using the established cell line of  claim 1 . 
     
     
         10 . The method according to  claim 9 , which comprises:
 introducing a foreign gene into a cell line established from distal tubular epithelial cells derived from a mammal to produce transformed cells;   culturing the obtained transformed cells, so as to allow the cells to produce a recombinant protein encoded by the foreign gene; and   recovering the produced recombinant protein.   
     
     
         11 . The method according to  claim 9 , wherein the produced recombinant protein is modified with a sugar chain. 
     
     
         12 . A recombinant protein, which is produced by the method of  claim 9 .